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Adaptive Optics in Multiphoton Microscopy

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Advanced Optical Methods for Brain Imaging

Part of the book series: Progress in Optical Science and Photonics ((POSP,volume 5))

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Abstract

Multiphoton (MP) microscopy provides inherent confocality and it has the ability to achieve three-dimensional imaging of thick samples. However, biological specimens are plenty of inhomogeneous structures that degrade and limit its performance. These are optically understood as specimen-induced aberrations and scattering, which negative influence in MP image quality increase with sample’s depth. Measurement and correction of aberrations are the goals of adaptive optics (AO) techniques. Although AO approaches were early used in conventional microscopes, it was only a question of time to have similar procedures implemented into MP imaging devices. A number of robust and efficient AO strategies have been implemented into MP microscopes, allowing these devices to provide high-resolution images of layers located at deeper locations within different biological samples, in particular brain tissue. Along this chapter, different AO MP approaches developed in the last two decades will be reviewed and discussed.

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Acknowledgements

This author thanks E. J. Gualda, M. Skorsetz, F. J. Ávila, and P. Artal for their help during the different MP experiments carried at the Laboratorio de Óptica of the Universidad de Murcia. Support from grants FIS2013-41237-R and FIS2016-76163-R is also acknowledged.

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Authors and Affiliations

  1. Centro de Investigación en Óptica y Nanofísica, Laboratorio de Óptica, Universidad de Murcia, Campus de Espinardo, 30100, Murcia, Spain

    Juan M. Bueno

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  1. Juan M. Bueno
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Correspondence to Juan M. Bueno .

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Editors and Affiliations

  1. Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan

    Fu-Jen Kao

  2. Department of Electrical and Computer Engineering, Boston University, Boston, Massachusetts, USA

    Gerd Keiser

  3. Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan

    Ankur Gogoi

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Bueno, J.M. (2019). Adaptive Optics in Multiphoton Microscopy. In: Kao, FJ., Keiser, G., Gogoi, A. (eds) Advanced Optical Methods for Brain Imaging. Progress in Optical Science and Photonics, vol 5. Springer, Singapore. https://doi.org/10.1007/978-981-10-9020-2_14

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  • DOI: https://doi.org/10.1007/978-981-10-9020-2_14

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