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Stimulated Raman Scattering Microscopy for Brain Imaging: Basic Principle, Measurements, and Applications

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Advanced Optical Methods for Brain Imaging

Part of the book series: Progress in Optical Science and Photonics ((POSP,volume 5))

Abstract

Stimulated Raman scattering (SRS) microscopy has proven to be a powerful imaging modality over the past decade due to its intrinsic capacity to provide a molecular fingerprint of the target specimen by detecting the vibrational energies associated with its chemical bonds. In fact, SRS automatically avoids the cumbersome process of attaching a fluorophore or fluorescence protein which may alter the intrinsic folding of the molecules due to its larger size and heavier molecular weight. Being a nonlinear imaging technique, SRS also enjoys other advantages such as pinhole-less three-dimensional optical sectioning, non-invasive observation, deep tissue penetration. Additionally, in contrast to coherent anti-Stokes Raman scattering (CARS), which is another coherent Raman technique, SRS signal is identical to spontaneous Raman spectra, linearly dependent on concentration, and free from non-resonant background. In this chapter, the basic principle of SRS microscopy and the corresponding advantages are elucidated. An overview of the advances in SRS measurements is also presented. Specifically, the recent progress in the instrumentation and chemistry related to both label-free and vibrational label-assisted SRS microscopy is reviewed with special emphasis on the brain imaging applications.

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Acknowledgements

We would like to acknowledge the Ministry of Science and Technology (MOST), Taiwan, and University Grants Commission (UGC), India, for their support to the biophotonics research projects at NYMU and JBC (UGC Grant No. F.5-376/2014-15/MRP/NERO/2181).

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Authors and Affiliations

  1. Institute of Biophotonics, National Yang-Ming University, Taipei, No.155, Sec.2, Linong Street, Taipei, 112, Taiwan

    Ankur Gogoi, Yi-Chih Liang & Fu-Jen Kao

  2. Department of Physics, Jagannath Barooah College, Jorhat, 785001, Assam, India

    Ankur Gogoi

  3. Department of Electrical and Computer Engineering, Boston University, 8 St. Mary’s St Boston, Boston, MA, 02215, USA

    Gerd Keiser

Authors
  1. Ankur Gogoi
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  2. Yi-Chih Liang
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  3. Gerd Keiser
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  4. Fu-Jen Kao
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Corresponding authors

Correspondence to Ankur Gogoi or Fu-Jen Kao .

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Editors and Affiliations

  1. Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan

    Fu-Jen Kao

  2. Department of Electrical and Computer Engineering, Boston University, Boston, Massachusetts, USA

    Gerd Keiser

  3. Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan

    Ankur Gogoi

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Gogoi, A., Liang, YC., Keiser, G., Kao, FJ. (2019). Stimulated Raman Scattering Microscopy for Brain Imaging: Basic Principle, Measurements, and Applications. In: Kao, FJ., Keiser, G., Gogoi, A. (eds) Advanced Optical Methods for Brain Imaging. Progress in Optical Science and Photonics, vol 5. Springer, Singapore. https://doi.org/10.1007/978-981-10-9020-2_10

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  • DOI: https://doi.org/10.1007/978-981-10-9020-2_10

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  • Online ISBN: 978-981-10-9020-2

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