Abstract
Pomacea canaliculata (PC) is one of the most destructive pests as it causes severe damage to the paddy field. Most of the approaches are targeting its flesh rather than its egg; however in the same time, it has high fecundity and hatchability rate. Thus, effective approach is necessary to control the hatchability, which in turn affects its life cycle. Protease has been shown to have the capability to suppress the hatchability of Pomacea canaliculata eggs (PCE). The objective of this study is to further support the findings, where it discusses the chemical assessment of protease-treated PCE. The assessment focuses on the effect of protease treatment on the PCE in terms of the following factors, namely non-hatchability, cuticle protein reduction, gas exchange and water loss activity. Non-hatchability of 42 and 91% after treating with 0.75 and 5 U/ml protease, respectively, was recorded. With these findings, it is safe to consider that protease applicable to be used as biocontrol against PCE. The non-hatchability of protease-treated eggs occurred as a result of cuticle disruption as supported by the present chemical studies.
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Acknowledgements
The researchers would like to thank the Malaysia Ministry of Science, Technology and Innovation (MOSTI) under Science Fund Grant (9005-00056) for financial support, the Perlis Department of Agriculture for the supporting snail invasion data and the School of Bioprocess Engineering, University Malaysia Perlis for their facilities and continuing support.
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Salleh, N.H.M., Zainalabidin, N. (2018). Evaluation of Protease Enzyme on Pomacea canaliculata Eggs. In: Saian, R., Abbas, M. (eds) Proceedings of the Second International Conference on the Future of ASEAN (ICoFA) 2017 – Volume 2. Springer, Singapore. https://doi.org/10.1007/978-981-10-8471-3_44
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DOI: https://doi.org/10.1007/978-981-10-8471-3_44
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