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The Production of Recombinant Proteins from Mammalian Cells Using RNA Element

  • Intisar Fouad Ali Mursi
  • Seiji Masuda
Chapter

Abstract

Producing recombinant proteins in a large scale for pharmaceutical use is a challenging process as these proteins must be posttranscriptionally modified. Mammalian cells have proven to be good candidates for this process to take place efficiently. In order to optimize gene expression of the required proteins in mammalian cells, good vectors must be used such as the viral vectors. Vectors must be chosen cautiously according to the type of the mammalian cell line being utilized. Importantly, strong promoters must be selected to ensure large amounts of the gene(s) of interest.

The export of the messenger ribonucleic acid (mRNA) is a complex process in which many proteins are involved. A strategy to enhance recombinant protein production is to use the mRNA export pathway efficiently. In the mRNA export pathway, key proteins include the NXF1-NXT1 heterodimer. Here we introduce the use of constitutive transport element in the expression system. Constitutive transport element directly recruits mRNA export proteins NXF1-NXT1, and these events facilitate the mRNA export containing constitutive transport element. The simultaneous overexpression of mRNA export factors in addition to the use of RNA element recruiting mRNA export proteins is a potential strategy to obtain satisfactory amounts of the required proteins.

Keywords

Recombinant protein production RNA export RNA element RNA-binding protein 

Notes

Acknowledgments

We would like to express our thanks to Lwando Moshani from Kyoto Seika University, Japan, for creating some of the drawings for this book chapter. This work was partially supported by JSPS KAKENHI Grant Number 17K19232 to SM.

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Division of Integrated Life Sciences, Graduate School of BiostudiesKyoto UniversityKyotoJapan

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