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Functional Nucleic Acid Based Biosensors for GMO Detection

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Functional Nucleic Acid Based Biosensors for Food Safety Detection
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Abstract

With the growing presence of genetically modified organisms (GMOs) in our daily lives, discussions and concerns about it have not ceased. Meanwhile, many different methods for GMO detection have been developed based on molecule or protein. And the functional nucleic acid has made an important contribution to the detection with its unique advantages.

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References

  1. G. Feriotto, M. Borgatti, C. Mischiati, N. Bianchi, R. Gambari, Biosensor technology and surface plasmon resonance for real-time detection of genetically modified Roundup Ready soybean gene sequences. J. Agric. Food Chem. 50(5), 955–962 (2002)

    Article  CAS  PubMed  Google Scholar 

  2. Z. Aghili, N. Nasirizadeh, A. Divsalar, S. Shoeibi, P. Yaghmaei, A nanobiosensor composed of exfoliated graphene oxide and gold nano-urchins, for detection of GMO products. Biosens. Bioelectron. 95, 72–80 (2017)

    Article  CAS  PubMed  Google Scholar 

  3. T.N. Truong, D.L. Tran, T.H. Vu, V.H. Tran, T.Q. Duong, Q.K. Dinh, T. Tsukahara, Y.H. Lee, J.S. Kim, Multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole DNA biosensor for label-free detection of genetically modified organisms by QCM and EIS. Talanta 80(3), 1164 (2010)

    Article  CAS  PubMed  Google Scholar 

  4. M. Wang, X. Du, L. Liu, Q. Sun, X. Jiang, DNA biosensor prepared by electrodeposited Pt-nanoparticles for the detection of specific deoxyribonucleic acid sequence in genetically modified soybean. Chin. J. Anal. Chem. 36(7), 890–894 (2008)

    Article  CAS  Google Scholar 

  5. M. Mix, J. Rüger, S. Krüger, I. Broer, G.-U. Flechsig, Electrochemical detection of 0.6% genetically modified maize MON810 in real flour samples. Electrochem. Commun. 22, 137–140 (2012)

    Article  CAS  Google Scholar 

  6. Z. Zheng, J. Hu, Z. He, A split G-quadruplex and graphene oxide-based low-background platform for fluorescence authentication of Pseudostellaria heterophylla. Sensors (Basel). 14(12), 22971–22981 (2014)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. X. Jiang, H. Zhang, J. Wu, X. Yang, J. Shao, Y. Lu, B. Qiu, Z. Lin, G. Chen, G-quadruplex DNA biosensor for sensitive visible detection of genetically modified food. Talanta 128, 445–449 (2014)

    Article  CAS  PubMed  Google Scholar 

  8. B. Qiu, Y. Zhang, Y. Lin, Y. Lu, Z. Lin, K. Wong, G. Chen, A novel fluorescent biosensor for detection of target DNA fragment from the transgene cauliflower mosaic virus 35S promoter. Biosens. Bioelectron. 41, 168–171 (2013)

    Article  CAS  PubMed  Google Scholar 

  9. N. Cheng, Y. Shang, Y. Xu, L. Zhang, Y. Luo, K. Huang, W. Xu, On-site detection of stacked genetically modified soybean based on event-specific TM-LAMP and a DNAzyme-lateral flow biosensor. Biosens. Bioelectron. 91, 408–416 (2017)

    Article  CAS  PubMed  Google Scholar 

  10. X. Huang, C. Zhai, Q. You, H. Chen, Potential of cross-priming amplification and DNA-based lateral-flow strip biosensor for rapid on-site GMO screening. Anal. Bioanal. Chem. 406(17), 4246–4249 (2014)

    Article  CAS  Google Scholar 

  11. W. Hemmer, Foods derived from genetically modified organisms and detection methods. Clin. Immunol. 127(400–401), S83 (1997)

    Google Scholar 

  12. Xiaodan Xu, Yingcong Li, Heng Zhao, Siyuan Wen, Shengqi Wang, Huang J, Kunlun Huang A, Yunbo Luo. Rapid and reliable detection and identification of GM events using multiplex PCR coupled with oligonucleotide microarray. J. Agric. Food Chem.. 2005; 53 (10):3789

    Article  CAS  PubMed  Google Scholar 

  13. M. Mendelsohn, J. Kough, Z. Vaituzis, K. Matthews, Are Bt crops safe? Nat. Biotechnol. 21(9), 1003–1009 (2003)

    Article  CAS  PubMed  Google Scholar 

  14. E. Anklam, F. Gadani, P. Heinze, H. Pijnenburg, E. Gvanden, Analytical methods for detection and determination of genetically modified organisms in agricultural crops and plant-derived food products. Eur. Food Res. Technol. 214(1), 3–26 (2002)

    Article  CAS  Google Scholar 

  15. J.C. Mieog, C.A. Howitt, J.P. Ral, Fast-tracking development of homozygous transgenic cereal lines using a simple and highly flexible real-time PCR assay. BMC Plant Biol. 13(1), 1–9 (2013)

    Article  CAS  Google Scholar 

  16. A. Zimmermann, J. Lüthy, U. Pauli, Event specific transgene detection in Bt11 corn by quantitative PCR at the integration site. LWT Food Sci. Technol. 33(3), 210–216 (2000)

    Article  CAS  Google Scholar 

  17. Y.G. Liu, R.F. Whittier, Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics 25(3), 674–681 (1995)

    Article  CAS  PubMed  Google Scholar 

  18. Y. Aihu, Y. Xu, Z. Changsong, Kewei, Event-specific qualitative and quantitative PCR detection of MON863 maize based upon the 3′ -transgene integration sequence. J. Cereal Sci. 43(2), 250–257 (2006)

    Article  CAS  Google Scholar 

  19. G.P. Pfeifer, S.D. Steigerwald, P.R. Mueller, B. Wold, A.D. Riggs, Genomic sequencing and methylation analysis by ligation mediated PCR. Science 246(4931), 810–813 (1989)

    Article  CAS  PubMed  Google Scholar 

  20. A. Holck, M. Vaïtilingom, L. Didierjean, K. Rudi, 5′-nuclease PCR for quantitative event-specific detection of the genetically modified Mon810 MaisGard maize. Eur. Food Res. Technol. 215(2), 182–182 (2002)

    Article  CAS  Google Scholar 

  21. T. Kohda, K. Taira, A simple and efficient method to determine the terminal sequences of restriction fragments containing known sequences. Dna Res. Int. J. Rapid Publ. Rep. Genes Genomes 7(2), 151 (2000)

    CAS  Google Scholar 

  22. A. Rosenthal, D.S. Jones, Genomic walking and sequencing by oligo-cassette mediated polymerase chain reaction. Nucleic Acids Res. 18(10), 3095 (1990)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Y. Ge, N.W. Charon, Identification of a large motility operon in Borrelia burgdorferi by semi-random PCR chromosome walking. Gene 189(2), 195 (1997)

    Article  CAS  PubMed  Google Scholar 

  24. Y. Yan, C. An, L. Li, J. Gu, G. Tan, Z. Chen, T-linker-specific ligation PCR (T-linker PCR): an advanced PCR technique for chromosome walking or for isolation of tagged DNA ends. Nucleic Acids Res. 31(12), e68 (2003)

    Article  Google Scholar 

  25. Q. Trinh, W. Xu, H. Shi, Y. Luo, K. Huang, An A-T linker adapter polymerase chain reaction method for chromosome walking without restriction site cloning bias. Anal. Biochem. 425(1), 62–67 (2012)

    Article  CAS  PubMed  Google Scholar 

  26. J. Riley, R. Butler, D. Ogilvie, R. Finniear, D. Jenner, S. Powell, R. Anand, J.C. Smith, A.F. Markham, A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones. Nucleic Acids Res. 18(10), 2887 (1990)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. P.N. Hengen, Vectorette, splinkerette and boomerang DNA amplification. Trends Biochem. Sci. 20(9), 372 (1995)

    Article  CAS  PubMed  Google Scholar 

  28. J.H. Shiming Wang, Z. Cui, S. Li, Self-formed adaptor PCR: a simple and efficient method for chromosome walking. Appl. Environ. Microbiol. 73(15), 5048 (2007)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. W. Xu, Y. Shang, P. Zhu, Z. Zhai, J. He, K. Huang, Y. Luo, Randomly broken fragment PCR with 5′ end-directed adaptor for genome walking. Sci. Rep. 3, 3465 (2013)

    Article  PubMed  PubMed Central  Google Scholar 

  30. C. Leoni, M. Volpicella, F.D. Leo, R. Gallerani, L.R. Ceci, Genome walking in eukaryotes. FEBS J. 278(21), 3953 (2011)

    Article  CAS  PubMed  Google Scholar 

  31. V. Thirulogachandar, P. Pandey, C.S. Vaishnavi, M.K. Reddy, An affinity-based genome walking method to find transgene integration loci in transgenic genome. Anal. Biochem. 416(2), 196–201 (2011)

    Article  CAS  PubMed  Google Scholar 

  32. D. Kovalic, The use of next generation sequencing and junction sequence analysis bioinformatics to achieve molecular characterization of crops improved through modern biotechnology. Plant Genome. 5(3), 149–163 (2012)

    CAS  Google Scholar 

  33. J. Shendure, H. Ji, Next-generation DNA sequencing. Nat. Biotechnol. 26(10), 1135 (2008)

    Article  CAS  PubMed  Google Scholar 

  34. P. Medvedev, M. Stanciu, M. Brudno, Computational methods for discovering structural variation with next-generation sequencing. Nat. Methods 6(11 Suppl), S13 (2009)

    Article  CAS  PubMed  Google Scholar 

  35. W. Hua, T. Caitlin, S. Blanchard, Z. Guan, The Fidelity Index provides a systematic quantitation of star activity of DNA restriction endonucleases. Nucleic Acids Res. 36(9), e50 (2008)

    Article  CAS  Google Scholar 

  36. Litao Yang, Songci Xu, Aihu Pan, Changsong Yin, Kewei Zhang, Wang Z, Zhigang Zhou A, Dabing Zhang. Event specific qualitative and quantitative polymerase chain reaction detection of genetically modified MON863 maize based on the 5′-transgene integration sequence. J. Agric. Food Chem. 2005; 53 (24):9312

    Article  CAS  PubMed  Google Scholar 

  37. B. Fca, F. Cdoss, L.L. Valente, A. Acm, Nested PCR detection of genetically modified soybean in soybean flour, infant formula and soymilk. LWT Food Sci. Technol. 40(4), 748–751 (2007)

    Article  CAS  Google Scholar 

  38. A.Z. Dinon, J.E.D. Melo, A.C.M. Arisi, Monitoring of MON810 genetically modified maize in foods in Brazil from 2005 to 2007. J. Food Compos. Anal. 21(6), 515–518 (2008)

    Article  CAS  Google Scholar 

  39. R. Higuchi, C. Fockler, G. Dollinger, R. Watson, Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Biotechnology 11(9), 1026 (1993)

    PubMed  CAS  Google Scholar 

  40. W.T. Xu, W.B. Bai, Y.B. Luo, Y.F. Yuan, K.L. Huang, Research progress in techniques for detecting genetically modified organisms. Chin. J. Agric. Biotechnol. 6(1), 1–9 (2009)

    Article  CAS  Google Scholar 

  41. I. Calves, High resolution melting analysis for fast and cheap polymorphism screening of marine populations. Nature Com. (2012)

    Google Scholar 

  42. M. Mazzara, A. Bogni, G. Van Den Eede, Event-specific method for the quantification of cotton line MON1445 using real-time PCR. (Publications Office of the European Union, 2008), pp. 1–87

    Google Scholar 

  43. L. Yang, A. Pan, K. Zhang, C. Yin, B. Qian, J. Chen, C. Huang, D. Zhang, Qualitative and quantitative PCR methods for event-specific detection of genetically modified cotton Mon1445 and Mon531. Transgenic Res. 14(6), 817–831 (2005)

    Article  CAS  PubMed  Google Scholar 

  44. W.T. Xu, N. Zhang, Y.B. Luo, Z.F. Zhai, Y. Shang, X.H. Yan, J.J. Zheng, K.L. Huang, Establishment and evaluation of event-specific qualitative and quantitative PCR method for genetically modified soybean DP-356043-5. Eur. Food Res. Technol. 233(4), 685 (2011)

    Article  CAS  Google Scholar 

  45. N. Zhang, W. Xu, W. Bai, Z. Zhai, Y. Luo, X. Yan, J. He, K. Huang, Event-specific qualitative and quantitative PCR detection of LY038 maize in mixed samples. Food Control 22(8), 1287–1295 (2011)

    Article  CAS  Google Scholar 

  46. A. Holck, M. Vaïtilingom, L. Didierjean, K. Rudi, 5′-nuclease PCR for quantitative event-specific detection of the genetically modified Mon810 MaisGard maize. Eur. Food Res. Technol. 214(5), 449–454 (2002)

    Article  CAS  Google Scholar 

  47. C. Collonnier, A. Schattner, G. Berthier, F. Boyer, G. Couéphilippe, A. Diolez, M.N. Duplan, S. Fernandez, N. Kebdani, A. Kobilinsky, Characterization and event specific-detection by quantitative real-time PCR of T25 maize insert. J. AOAC Int. 88(2), 536–546 (2005)

    PubMed  CAS  Google Scholar 

  48. C.R. Nielsen, K.G. Berdal, A. Holst-Jensen, Characterisation of the 5′ integration site and development of an event-specific real-time PCR assay for NK603 maize from a low starting copy number. Eur. Food Res. Technol. 219(4), 421–427 (2004)

    Article  CAS  Google Scholar 

  49. B.J. Hindson, K.D. Ness, D.A. Masquelier, P. Belgrader, N.J. Heredia, A.J. Makarewicz, I.J. Bright, M.Y. Lucero, A.L. Hiddessen, T.C. Legler, High-throughput droplet digital PCR system for absolute quantitation of DNA copy number. Anal. Chem. 83(22), 8604 (2011)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  50. S. Bhat, J. Herrmann, P. Armishaw, P. Corbisier, K.R. Emslie, Single molecule detection in nanofluidic digital array enables accurate measurement of DNA copy number. Anal. Bioanal. Chem. 394(2), 457–467 (2009)

    Article  CAS  PubMed  Google Scholar 

  51. A.S. Whale, J.F. Huggett, S. Cowen, V. Speirs, J. Shaw, S. Ellison, C.A. Foy, D.J. Scott, Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation. Nucleic Acids Res. 40(11), e82 (2012)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  52. M.J. Burns, A.M. Burrell, C.A. Foy, The applicability of digital PCR for the assessment of detection limits in GMO analysis. Eur. Food Res. Technol. 4(3), 43–53 (1999)

    Google Scholar 

  53. M. Baker, Digital PCR hits its stride. Nat. Methods 9(9), 541–544 (2012)

    Article  CAS  Google Scholar 

  54. G.P. Mcdermott, D. Do, C.M. Litterst, D. Maar, C.M. Hindson, E.R. Steenblock, T.C. Legler, Y. Jouvenot, S.H. Marrs, A. Bemis, Multiplexed target detection using DNA-binding dye chemistry in droplet digital PCR. Anal. Chem. 85(23), 11619–11627 (2013)

    Article  CAS  PubMed  Google Scholar 

  55. T. Notomi, H. Okayama, H. Masubuchi, T. Yonekawa, K. Watanabe, N. Amino, T. Hase, Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28(12), E63 (2000)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  56. F. Maruyama, T. Kenzaka, N. Yamaguchi, K. Tani, M. Nasu, Detection of bacteria carrying the stx2 gene by in situ loop-mediated isothermal amplification. Appl. Environ. Microbiol. 69(8), 5023 (2003)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  57. G. Zhang, E.W. Brown, N. Gonzálezescalona, Comparison of real-time PCR, reverse transcriptase real-time PCR, loop-mediated isothermal amplification, and the FDA conventional microbiological method for the detection of salmonella spp. in produce. Appl. Environ. Microbiol. 77(18), 6495–6501 (2011)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  58. X.L. Xi Lu, Z. Mo, F. Jin, B. Wang, H. Zhao, X. Shan, L. Shi, Rapid identification of Chikungunya and Dengue virus by a real-time reverse transcription-loop-mediated isothermal amplification method. Am J Trop Med Hyg. 87(5), 947–953 (2012)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  59. A. Ablordey, D.A. Amissah, I.F. Aboagye, B. Hatano, T. Yamazaki, T. Sata, K. Ishikawa, H. Katano, Detection of mycobacterium ulcerans by the loop mediated isothermal amplification method. PLoS Negl. Trop. Dis. 6(4), e1590 (2012)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  60. C. Zahradnik, C. Kolm, R. Martzy, R.L. Mach, R. Krska, A.H. Farnleitner, K. Brunner, Detection of the 35S promoter in transgenic maize via various isothermal amplification techniques: a practical approach. Anal. Bioanal. Chem. 406(27), 6835–6842 (2014)

    Article  CAS  PubMed  Google Scholar 

  61. J. Xu, Q. Zheng, L. Yu, R. Liu, X. Zhao, G. Wang, Q. Wang, J. Cao, Loop-mediated isothermal amplification (LAMP) method for detection of genetically modified maize T25. Food Sci. Nutr. 1(6), 432–438 (2013)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  62. X. Huang, L. Chen, J. Xu, H.F. Ji, S. Zhu, H. Chen, Rapid visual detection of phytase gene in genetically modified maize using loop-mediated isothermal amplification method. Food Chem. 156(3), 184 (2014)

    Article  CAS  PubMed  Google Scholar 

  63. F. Li, W. Yan, L. Long, X. Qi, C. Li, S. Zhang, Development and application of loop-mediated isothermal amplification assays for rapid visual detection of cry2Ab and cry3A genes in genetically-modified crops. Int. J. Mol. Sci. 15(9), 15109–15121 (2014)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  64. X.J. Ma, Y.L. Shu, K. Nie, M. Qin, D.Y. Wang, R.B. Gao, M. Wang, L.Y. Wen, F. Han, S.M. Zhou, Visual detection of pandemic influenza A H1N1 Virus 2009 by reverse-transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye. J. Virol. Methods 167(2), 214–217 (2010)

    Article  CAS  PubMed  Google Scholar 

  65. L. Luo, K. Nie, M.J. Yang, M. Wang, J. Li, C. Zhang, H.T. Liu, X.J. Ma, Visual detection of high-risk human papillomavirus genotypes 16, 18, 45, 52, and 58 by loop-mediated isothermal amplification with Hydroxynaphthol blue dye. J. Clin. Microbiol. 49(10), 3545 (2011)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  66. S. Fukuta, Y. Mizukami, A. Ishida, J. Ueda, M. Hasegawa, I. Hayashi, M. Hashimoto, M. Kanbe, Real-time loop-mediated isothermal amplification for the CaMV-35S promoter as a screening method for genetically modified organisms. Eur. Food Res. Technol. 218(5), 496–500 (2004)

    Article  CAS  Google Scholar 

  67. S. Huang, Y. Xu, X. Yan, Y. Shang, P. Zhu, W. Tian, W. Xu, Development and application of a quantitative loop-mediated isothermal amplification method for detecting genetically modified maize MON863. J. Sci. Food Agric. 95(2), 253–259 (2015)

    Article  CAS  PubMed  Google Scholar 

  68. X.U. Wen-Tao, K.L. Huang, Y.B. Luo, SYBR Green I based PCR for detection of the bar and pat genes in genetically modified organisms. Food Sci. 27(3), 202–206 (2006)

    Google Scholar 

  69. H.Y.H. And, T.M. Pan, Detection of genetically modified maize MON810 and NK603 by multiplex and real-time polymerase chain reaction methods. J. Agric. Food Chem. 52(11), 3264–3268 (2004)

    Article  CAS  Google Scholar 

  70. A. Germini, A. Zanetti, C. Salati, S. Rossi, C. Forré, S. Schmid, R. Marchelli, C. Fogher, Development of a seven-target multiplex PCR for the simultaneous detection of transgenic soybean and maize in feeds and foods. J. Agric. Food Chem. 52(11), 3275–3280 (2004)

    Article  CAS  PubMed  Google Scholar 

  71. W. Xu, Z. Zhai, K. Huang, N. Zhang, Y. Yuan, Y. Shang, Y. Luo, A novel universal primer-multiplex-PCR method with sequencing gel electrophoresis analysis. PLoS One 7(1), e22900 (2012)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  72. W. Xu, Y. Yuan, Y. Luo, W. Bai, C. Zhang, K. Huang, Event-specific detection of stacked genetically modified maize Bt11 x GA21 by UP-M-PCR and real-time PCR. J. Agric. Food Chem. 57(2), 395 (2009)

    Article  CAS  PubMed  Google Scholar 

  73. W.T. Xu, W.B. Bai, Y.B. Luo, Y. Yuan, W. Zhang, X. Guo, K. Huang, A novel common single primer multiplex polymerase chain reaction (CSP-M-PCR) method for the identification of animal species in minced meat. J. Sci. Food Agric. 88(15), 2631–2637 (2008)

    Article  CAS  Google Scholar 

  74. C. Zhang, W. Xu, Z. Zhai, Y. Luo, X. Yan, N. Zhang, K. Huang, Universal primer-multiplex-polymerase chain reaction (UP-M-PCR) and capillary electrophoresis-laser-induced fluorescence analysis for the simultaneous detection of six genetically modified maize lines. J. Agric. Food Chem. 59(10), 5188–5194 (2011)

    Article  CAS  PubMed  Google Scholar 

  75. J. Guo, L. Yang, L. Chen, D. Morisset, X. Li, L. Pan, D. Zhang, MPIC: a high-throughput analytical method for multiple DNA targets. Anal. Chem. 83(5), 1579–1586 (2011)

    Article  CAS  PubMed  Google Scholar 

  76. L. Véronèse, O. Tournilhac, P. Combes, N. Prie, E. Pierre-Eymard, R. Guièze, R. Veyrat-Masson, J.O. Bay, P. Vago, A. Tchirkov, Contribution of MLPA to routine diagnostic testing of recurrent genomic aberrations in chronic lymphocytic leukemia. Cancer Gene Ther. 206(1–2), 19–25 (2013)

    Article  CAS  Google Scholar 

  77. J. Cui, M. Azimi, A.D. Adekile, A.H. Al, C.C. Hoppe, Detection of anti-Lepore Hb P-Nilotic by multiplex ligation-dependent probe amplification. Hemoglobin 36(3), 276–282 (2012)

    Article  CAS  PubMed  Google Scholar 

  78. F. Moreano, A. Ehlert, U. Busch, K.H. Engel, Ligation-dependent probe amplification for the simultaneous event-specific detection and relative quantification of DNA from two genetically modified organisms. Eur. Food Res. Technol. 222(5–6), 479–485 (2006)

    Article  CAS  Google Scholar 

  79. H. AL, D. SM, EH, Quantitative, multiplex ligation-dependent probe amplification for the determination of eight genetically modified maize events. Eur. Food Res. Technol. 230(2), 185–194 (2009)

    Article  CAS  Google Scholar 

  80. E. Alexandra, M. Francisco, B. Ulrich, E. Karlheinz, Development of a modular system for detection of genetically modified organisms in food based on ligation-dependent probe amplification. Eur. Food Res. Technol. 227(3), 805–812 (2008)

    Article  CAS  Google Scholar 

  81. Y. Shang, P. Zhu, W. Xu, T. Guo, W. Tian, Y. Luo, K. Huang, Single universal primer multiplex ligation-dependent probe amplification with sequencing gel electrophoresis analysis. Anal. Biochem. 443(2), 243–248 (2013)

    Article  CAS  PubMed  Google Scholar 

  82. H.K. Shrestha, K.K. Hwu, S.J. Wang, L.F. Liu, M.C. Chang, Simultaneous detection of eight genetically modified maize lines using a combination of event- and construct-specific multiplex-PCR technique. J. Agric. Food Chem. 56(19), 8962–8968 (2008)

    Article  CAS  PubMed  Google Scholar 

  83. T. Lalic, R.H. Vossen, J. Coffa, J.P. Schouten, M. Gucscekic, D. Radivojevic, M. Djurisic, M.H. Breuning, S.J. White, J.T. den Dunnen, Deletion and duplication screening in the DMD gene using MLPA. Eur. J. Hum. Genet. Ejhg 13(11), 1231 (2005)

    Article  CAS  PubMed  Google Scholar 

  84. Q. Lan, Tianiin. Special-base GMCs detection using multiple PCR and pyrosequencing. J. China Agric. Univ. (2012)

    Google Scholar 

  85. Q. Song, G. Wei, G. Zhou, Analysis of genetically modified organisms by pyrosequencing on a portable photodiode-based bioluminescence sequencer. Food Chem. 154(154), 78 (2014)

    Article  CAS  PubMed  Google Scholar 

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Luo, Y. (2018). Functional Nucleic Acid Based Biosensors for GMO Detection. In: Functional Nucleic Acid Based Biosensors for Food Safety Detection. Springer, Singapore. https://doi.org/10.1007/978-981-10-8219-1_3

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