Estimation of Inorganic Phosphorus in Serum and Urine

  • Vijay Kumar
  • Kiran Dip Gill


The body contains about 800 g of phosphorus. Of this, 90% is present in bones, so it is more widely distributed in the body than calcium. In the soft tissues, phosphorus is present as organic form and incorporated into macromolecules. Phosphorous is also present in some proteins, lipids, and nucleic acid and in some coenzymes. Phosphorus also plays a role in acid-base regulation of body fluids particularly by the kidneys. The phosphorus of the blood is of four types:
  1. 1.

    Inorganic phosphorus – the phosphates of alkaline and alkali earth metals, present as H2PO4 and \( {\mathrm{HPO}}_4^{2-} \)

  2. 2.

    Organic or ester phosphorus – including glycerophosphates, nucleotide phosphate, hexose phosphate, etc.

  3. 3.

    Lipid phosphorus – present in lecithin, cephalin, sphingomyelin

  4. 4.

    Residual phosphorus present in small amounts


Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Vijay Kumar
    • 1
  • Kiran Dip Gill
    • 2
  1. 1.Department of BiochemistryMaharshi Dayanand UniversityRohtakIndia
  2. 2.Department of BiochemistryPostgraduate Institute of Medical Education & ResearchChandigarhIndia

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