Abstract
Orchids are among the elite of flowering plants, fetching a very high price in the international market. The orchid industry of today is dependent on micropropagation for planting material, since orchid seeds lack a functional endosperm and do not germinate naturally without a fungal association. Proper growth and maintenance of orchid plantlets in tissue culture require an external supply of growth regulators. Recently, thidiazuron (TDZ) has surfaced as an effective bioregulant in plant cell and tissue cultures by virtue of its morpho-regulatory potential. It is reported to be the matchless synthetic cytokinin available and has significantly improved the in vitro regeneration and multiplication of species recalcitrant to propagation. This chapter summarizes the various developments and recent advancements in the application of TDZ for tissue culture of orchids.
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References
Aung HN, Park IS, Hwang YJ, Chung JD, Lim KB (2010) In vitro micropropagation and conservation of Rhynchostylis retusa BL. Hortic Environ Biotechnol 51:440–444
Balilashaki K, Ruhangiz N, Siamak K, Aboozar S (2014) Micropropagation of Phalaenopsis amabilis cv. Cool ‘Breeze’ with using of flower stalk nodes and leaves of sterile obtained from node cultures. Int J Farm Allied Sci 3:823–829
Bastin M, Jeyachandran R (2015) Ex situ conservation of trichoglottis tenera (Lindl.) a threatened, and endangered orchid of western ghats using asymbiotic seed germination technique. Int J Recent Sci Res 6:3488–3496
Bhattacharyya P, Suman K, Reemavareen D, Pramod T (2014) Genetic stability and phytochemical analysis of the in vitro regenerated plants of Dendrobium nobile Lindl., an endangered medicinal orchid. Meta Gene 2:489–504
Deepak KS, Shashi BB (2016) In vitro propagation and chemical profiling of Herminium lanceum (Thunb. ex Sw.) Vuijk, a medicinally important orchid, for therapeutically important phenolic acids. Plant Biotechnol 33:153–160
Dini M, Iriawati (2016) Regeneration of plantlets through PLB (protocorm-like body) formation in Phalaenopsis ‘Join Angle X Sogo Musadian’. J Math Fund Sci 48:204–212
Gangadhar S, Mulgund K, Nataraja RB, Malabadi S, Kumar V (2011) TDZ induced in vitro propagation of an epiphytic orchid Xenikophyton smeeanum (Reichb. f.) Res Plant Biol 4:07–15
Hajong S, Kumaria S, Tandon PJ (2013) Effect of plant growth regulators on regeneration potential of axenic nodal segments of Dendrobium chrysanthum wall. Ex Lindl Agr Sci Tech 15:1425–1435. http://sites.psu.edu/orchidtissueculture/2014/03/22/dendrobium-kingianum-tissue-culture-propagation/
Jitsopakul N, Kanchit T, Keiko I (2013) Efficient adventitious shoot regeneration from shoot tip culture of Vanda coerulea, a Thai orchid. Sci Asia 39:449–455
Jones MP, Cao J, O’Brien R, Murch SJ, Saxena PK (2007) The mode of action of thidiazuron: auxins, indoleamines, and ion channels in the regeneration of Echinacea purpurea L. Plant Cell Rep 26:1481–1490
Kou Y, Cunquan Y, Qingcui Z, Guoqin L, Jing N, Zhimin M, Chenxia C, Teixeira da Silva JA, Liangjun Z (2016) Thidiazuron triggers morphogenesis in Rosa canina L. Protocorm-like bodies by changing incipient cell fate. Front Plant Sci 7:557
Latip MA, Rosmah M, Zaleha AA, Lai HT, Lilly MG, Rimi R (2010) Effects of N6 -Benzyladenine and Thidiazuron on proliferation of Phalaenopsis gigantea Protocorms. Asia Pac J Mol Biol Biotechnol 18:215–218
Lee Y-I, Lee N (2003) Plant regeneration from protocorm-derived callus of Cypripedium Formosanum. In Vitro Cell Dev Biol Plant 39:475–479
Liam Farrell (2014) http://sites.psu.edu/horticulture202blueberrypropagation/2014/03/22/dendrobium-kingianum-tissue-culture-propagation/#respond
Malabadi BR, Mulgund GS, Nataraja K (2004) Efficient Regeneration of Vanda coerulea, an Endangered Orchid Using Thidiazuron. Plant Cell Tissue Org Cult 76:289–293
Murthy BNS, Murch SJ, Saxena PK (1998) Thidiazuron: a potent regulator of in vitro plant morphogenesis. In Vitro Cell Dev Biol Plant 34:267–275
Natarajan K (2009) An in vitro study on micropropagation of Cymbidium orchids. Curr Biotica 3:244–250
Nihar RN, Shiba PR, Satyanarayan P, Nihar RN, Shiba PR, Satyanarayan P (1997) In vitro propagation of three epiphytic orchids, Cymbidium aloifolium (L.) Sw., Dendrobium aphyllum (Roxb.) Fisch. and Dendrobium moschatum (Buch-Ham) Sw. through thidiazuron-induced high frequency shoot proliferation. Sci Hortic 71:243–250
Panjan S, Kamnoon K (2011) Efficient direct protocorm-like bodies induction of dwarf Dendrobium using Thidiazuron. Not Sci Biol 3:88–92
Rachmawati BW, Nurhajati AM, Ni Made AW, Agus P (2015) Shoot tips derived-somatic embryogenesis in mass propagation of Dendrobium Indonesia Raya ‘Ina’ Fitri. Emirates J Food Agric 27:1–10
Samarfard S, Mihdzar AK, Saleh BK, Halimi MS, Seyed AR (2014) In vitro propagation and detection of somaclonal variation in Phalaenopsis gigantea as affected by chitosan and thidiazuron combinations. Hortscience 49:82–88
Sebastinraj J, John Britto S, Vinoth Kumar D, Philip Robinson J, Thangavel P (2014) Rapid propagation of Vanda testacea (Lindl.) Rchb.F. – a highly medicinal value epiphytic orchid of India. World J Agric Sci 10:223–230
Tan TK, Loon WS, Khor E, Loh CS (1998) Infection of Spathoglottis plicata (Orchidaceae) seeds by mycorrhizal fungus. Plant Cell Rep 18(1-2):14–19
Tao Jun LYFK, Zhao D (2011) Effects of plant growth regulators on in vitro propagation of Cymbidium faberi Rolfe. Afr J Biotechnol 10:15639–15646
Teixeira da Silva JA (2012) New basal media for protocorm-like body and callus induction of hybrid Cymbidium. J Fruit Ornamental Plant Res 20:127–133
Utami ESW, Hariyanto S (2016) The effect of organic nutrient and growth regulators on seed germination, embryo and shoots development of Dendrobium antennatum Lindl. Orchid by in vitro. Biosaintifika J Biol Biol Educ 8:165–171
Vacin EF, Went FW (1949) Some pH Changes in Nutrient Solutions. Bot Gaz 110(4):605–613
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Mohanraj, R. (2018). Role of Thidiazuron in Tissue Culture of Orchids. In: Ahmad, N., Faisal, M. (eds) Thidiazuron: From Urea Derivative to Plant Growth Regulator. Springer, Singapore. https://doi.org/10.1007/978-981-10-8004-3_26
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DOI: https://doi.org/10.1007/978-981-10-8004-3_26
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