Effect of TDZ on Various Plant Cultures

  • Sandeep R. Pai
  • Neetin S. Desai


This chapter provides the effect of thidiazuron (TDZ) on various plant cultures. Plant cell cultures still remain to be of great benefit to many disciplines including studies, viz., physiology, mechanism, etc. Apart from plant potency, this supremacy can be attributed to the increase in number of plant growth regulators (PGRs). Growth regulators are the mile stones in plant tissue culture history. Plant growth regulators depict some interesting functions; they singly, in synergy or antagonistically, function in growth of plant. Also, their concentrations play pivotal role in plant response. These PGRs are categorized in one of the five classes of plant hormones: auxins, gibberellins (GAs), cytokinins (CKs), ethylene (C2H4), and abscisic acid (ABA). In recent years apart from natural PGRs available, different synthetic PGRs are made available. The use of thidiazuron (N-phenyl-N′-1,2,3-thiadiazol-5-ylurea) has been successfully demonstrated to promote axillary shoot proliferation and to encourage shoot formation in plants. Recalcitrant woody species have been great responders to TDZ, reason being its high cytokinin-like activity and better response. It facilitates initiation of multiple shoots in many recalcitrant woody tree species. It has been observed that lower concentrations (<1 μM) of TDZ show greater axillary proliferation compared to other cytokinins. Besides, it has many adverse effects on culture, viz., higher concentration of TDZ causes no shoot elongation. Thus, the present chapter reveals the effect of TDZ on various plant cultures.


Thidiazuron Cytokinin Auxin Plant growth regulator Recalcitrant Woody 



Authors are indebted to the Head of Amity University, Mumbai.


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© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Amity Institute of Biotechnology (AIB)Amity UniversityBhatan, Post – Somathne, Panvel, MumbaiIndia

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