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Shoot Organogenesis of Aloe Plants with Emphasis on TDZ

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Thidiazuron: From Urea Derivative to Plant Growth Regulator

Abstract

Aloe species particularly Aloe vera (L.) is a potential crop in pharmaceutical, cosmetic and food industries. As a result of increasing demand for aloe, large-scale plant production is needed for commercial cultivation. Hence, in vitro plant propagation is necessary for its commercial production due to male sterility and limited number of conventionally produced propagules. In aloe in vitro propagation, adenine-type cytokinins, particularly BAP, are mostly used for shoot organogenesis. According to the literature survey, TDZ at low concentration is required for a short period to induce multiple shoot buds than BAP. However, TDZ is not commonly used in aloe plant production although TDZ has been used in plant tissue culture of the other medicinal plant and horticultural crop species. Hence, it could be incorporated into culture medium even with other cytokinins, normally BAP, at appropriate concentration. Addition of auxins particularly IAA and NAA may enhance the initiation of shoots. Thus, TDZ would utilize in aloe in vitro clonal propagation technique which is useful in mass plant production, genetic transformation and germplasm conservation for international germplasm exchange.

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References

  • Abdi G, Hedayat M, Modarresi M (2013) In vitro micropropagation of Aloe vera -impacts of plant growth regulators, media and type of explants. J Biol Environ Sci 7(19):19–24

    Google Scholar 

  • Abrie A, Staden JV (2001) Micropropagation of endangered Aloe polyphylla. Plant Growth Regul 33(1):19–23

    Article  CAS  Google Scholar 

  • Adelberg J, Adelberg JN (2012) Effects of cytokinin on multiplication and rooting of Aloe barbadensis during micropropagation on agar and liquid medium. J Med Active Plant 1(1):1–5

    Google Scholar 

  • Aggarwal D, Barna KS (2004) Tissue culture propagation of elite plant of Aloe vera Linn. J Plant Biochem Biotech 13(1):77–79

    Article  Google Scholar 

  • Ahmed S, Kabir AH, Ahmed MB, Razvy MA, Ganesan S (2007) Development of rapid micropropagation method of Aloe vera L. Sjemenarstvo 24(2):121–128

    Google Scholar 

  • Aina O, Quesenberry K, Gallo M (2012) Thidiazuron-induced tissue culture regeneration from quartered-seed explants of Arachis paraguariensis. Crop Sci 52(3):1076–1083

    CAS  Google Scholar 

  • Alvarenga ICA, Silva ST, Bertolucci SKV, Pinto JEBP, Pacheco FV (2015) Application of Thidiazuron (TDZ) for in vitro multiplication of yarrow (Achillea millefolium L.) and profile of volatile compounds. Aust J Crop Sci 9(10):948–953

    Google Scholar 

  • Babaei N, Abdullah NAP, Saleh G, Abdullah TL (2014) An efficient in vitro plantlet regeneration from shoot tip cultures of Curculigo latifolia, a medicinal plant. Sci World J. https://doi.org/10.1155/2014/275028

  • Baksha R, Jahan MAA, Khatun R, Munshi JL (2005) Micropropagation of Aloe barbadensis Mill through in vitro culture of shoot tip explants. Plant Tissue Cult Biotech 15(2):121–126

    Google Scholar 

  • Bhandari AK, Negi JS, Bisht VK, Bharti MK (2010) In vitro propagation of Aloe vera-A plant with medicinal properties. Nat Sci 8(8):174–176

    Google Scholar 

  • Bhatt ID, Dhar U (2004) Factors controlling micropropagation of Myrica esculenta buch.-Ham.ex D. Don. A high value wild edible of Kumaun Himalaya. Afr J Biotech 3(10):534–540

    Article  CAS  Google Scholar 

  • Blume T, Neis U (2005) Improving chlorine disinfection of wastewater by ultrasound application. Water Sci Tech 52(10–11):139–144

    CAS  Google Scholar 

  • Campestrini LH, Kuhnen S, Lemos PMM, Bach DB, Dias PF, Maraschin M (2006) Cloning protocol of Aloe vera as a study-case for “tailor-made” biotechnology to small farmers. J Technol Manag Innov 1(5):76–79

    Google Scholar 

  • Capasso F, Borrelli F, Capasso R (1998) Aloe and its therapeutics use. Phytother Res 12(S1):121–127

    Article  Google Scholar 

  • Cappelletti R, Sabbadini S, Mezzetti B (2016) The use of TDZ for the efficient in vitro regeneration and organogenesis of strawberry and blueberry cultivars. Sci Hortic 207:117–124. https://doi.org/10.1016/j.scienta.2016.05.016

    Article  CAS  Google Scholar 

  • Choudhary AK, Ray AK, Jha S, Mishra IN (2011) Callus formation, shoot initiation and in vitro culture of Aloe vera. Biotechnol Bioinformatics Bioeng 1(4):551–553

    Google Scholar 

  • Daneshvar MH, Moallemi N, Zadeh NA (2013) The effect of different media on shoot proliferation from the shoot tip of Aloe vera L. Jundishapur J Nat Pharm Prod 8(2):93–97

    Article  PubMed  PubMed Central  Google Scholar 

  • Debiasi C, Silva CG, Pescador R (2007) Micropropagation of Aloe vera L. Rev Bras Plant Med Botucatu 9(1):36–43

    CAS  Google Scholar 

  • Debnath SC (2005) Strawberry sepal: another explant for thidiazuron induced adventitious shoot regeneration. In Vitro Cell Dev Biol Plant 41(5):671–676

    Article  Google Scholar 

  • Dwivedi NK, Indiradevi A, Suma A, Nair RA (2014) Rapid in vitro micropropagation of non-bitter vegetable type Aloe vera L. (IC333202). Res Plant Biol 4(4):39–43

    Google Scholar 

  • Faisal M, Ahmad N, Anis M (2005) Shoot multiplication in Rauvolfia tetraphylla L using thidiazuron. Plant Cell Tissue Organ Cult 80(2):187–190

    Article  CAS  Google Scholar 

  • Fuentes SRL, Calheiros MBP, Manetti-Filho J, Vieira LGE (2000) The effects of silver nitrate and different carbohydrate sources on somatic embryogenesis in Coffea canephora. Plant Cell Tissue Organ Cult 60(1):5–13

    Article  CAS  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50(1):151–158

    Article  CAS  PubMed  Google Scholar 

  • Garro-Monge G, Gatica-Arias AM, Valdez-Melara M (2008) Somatic embryogenesis, plant regeneration and acemannan detection in aloe (Aloe barbadensis Mill.) Agron Costarric 32(2):41–52

    Google Scholar 

  • Genkov T, Iordanka I (1995) Effect of cytokinin-active phenylurea derivatives on shoot multiplication, peroxidase and superoxide dismutase activities of in vitro cultured carnation. Bulg J Plant Physiol 21(1):73–83

    CAS  Google Scholar 

  • Gordon MC, David JN (2001) Natural product drug discovery in the next millennium. Pharm Biol 39(1):8–17

    Article  Google Scholar 

  • Guo B, Abbasi BH, Zeb A, Xu LL, Weib YH (2011) Thidiazuron: a multi-dimensional plant growth regulator. Afr J Biotech 10(45):8984–9000

    Article  CAS  Google Scholar 

  • Gürel S, Topal E, Gürel E (2003) The effect of pretreating seedlings with TDZ on direct shoot regeneration from petiole explants of sugar beet (Beta vulgaris L.) Asia Pac J Mol Biol Biotechnol 11(1):57–62

    Google Scholar 

  • Haque SKM, Ghosh B (2013) High frequency microcloning of Aloe vera and their true-to-type conformity by molecular cytogenetic assessment of two years old field growing regenerated plants. Bot Stud 54:46. http://www.as-botanicalstudies.com/content/54/1/46

  • Hashemabadi D, Kaviani B (2008) Rapid micropropagation of Aloe vera L. via shoot multiplication. Afr J Biotech 7(12):1899–1902

    Article  CAS  Google Scholar 

  • Hiremath RC (2006) Micro propagation of ginger (Zingiber officinale Rosc.). M.Sc. thesis, College of Agriculture, Dharwad University of Agricultural Sciences, Dharwad, India

    Google Scholar 

  • Hosseini R, Parsa M (2007) Micropropagation of Aloe vera L. grown in South Iran. Pak J Biol Sci 10(7):1134–1137

    Article  CAS  PubMed  Google Scholar 

  • Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult 33(2):105–119

    Article  CAS  Google Scholar 

  • Ivanova MV (2009) Regulation of hyperhydricity in Aloe polyphylla propagated in vitro. Ph.D dissertation, School of Biological and Conservation Sciences, University of KwaZulu-Natal, South Africa

    Google Scholar 

  • Jones K, Aloecorp (2005) The antidiabetic activity of Aloe vera. Cosmet Sci Technol 2:34–35

    Google Scholar 

  • Kalimuthu K, Vijayakumar S, Senthilkumar RR, Sureshkumar M (2010) Micropropagation of Aloe vera Linn. A medicinal plant. Int J Biotechnol Biochem 6(3):405–410

    Google Scholar 

  • Kumar M, Singh S, Singh S (2011) In vitro morphogenesis of a medicinal plant–Aloe vera L. Asian J Plant Sci Res 1(1):31–40

    CAS  Google Scholar 

  • Kusula K, Prasad S, Umate P, Gadidasu K, Abbagani S (2008) Efficient TDZ and IAA assisted plant regeneration from cotyledon and leaf explants of Capsicum annum L. – one step protocol for shoot bud differentiation and elongation. Int J Plant Dev Biol 2(2):114–117

    Google Scholar 

  • Kyozuka J (2007) Control of shoot and root meristems function by cytokinin. Curr Opin Plant Biol 10(5):442–446

    Article  CAS  PubMed  Google Scholar 

  • Lavakumaran L, Seran TH (2014) Effect of 6-benzyl-aminopurine and thidiazuron on in vitro shoot organogenesis of Aloe vera (L.) Burm. f. Chil J Agric Res 74(4):497–501

    Article  Google Scholar 

  • Lavanya L, Seran TH (2013) Microshoot formation from shoot tip of Aloe vera (L.) under in vitro conditions. In: Proceeding of 2nd international symposium on minor fruits and medicinal plants for better lives, University of Ruhuna, Sri Lanka, 20 December 2013

    Google Scholar 

  • Ledbetter DI, Preece JE (2004) Thidiazuron stimulates adventitious shoot production from Hydrangea quercifolia Bartr. leaf explants. Sci Hortic 101(01–02):121–126

    Article  CAS  Google Scholar 

  • Liao Z, Chen M, Tan F, Sun X, Tang K (2004) Micropropagation of endangered Chinese aloe. Plant Cell Tissue Organ Cult 76(1):83–86

    Article  CAS  Google Scholar 

  • Lincy A, Sasikumar B (2010) Enhanced adventitious shoot regeneration from aerial stem explants of ginger using TDZ and its histological studies. Turk J Bot 34(1):21–29

    CAS  Google Scholar 

  • Liu X, Li J, Zhang Y, Li L, He D (2011) Biological research advancement in Aloe. J Med Plant Res 5(7):1046–1052

    Google Scholar 

  • Lobine D, Soulange JG, Sanmukhiya MR, Lavergne C (2015) A tissue culture strategy towards the rescue of endangered mascarene aloes. ARPN J Agric Biol Sci 10(1):28–38

    Google Scholar 

  • Long WX, Yang XB, Qi MY, Li DH, Guo T (2007) A review of research in tissue culture of Aloe. Subtrop Plant Sci 36(1):70–74

    Google Scholar 

  • Marfori EC, Malasa AB (2005) Tissue culture for the rapid clonal propagation of Aloe barbadensis Mill. Philipp Agric Sci 88(1):167–170

    Google Scholar 

  • Matand K, Prakash CS (2007) Evaluation of peanut genotypes for in vitro plant regeneration using thidiazuron. J Biotechnol 130(2):202–207

    Article  CAS  PubMed  Google Scholar 

  • Mok MC, Mok DWS, Armstrong DJ, Shudo K, Isogai Y, Okamoto T (1982) Cytokinin activity of N-phenyl-N′-1,2,3-thidiazol-5-yl urea (thidiazuron). Phytochemistry 21(7):1509–1511

    Article  CAS  Google Scholar 

  • Mok MC, Martin RC, Mok DWS (2000) Cytokinins: biosynthesis, metabolism and perception. In Vitro Cell Dev Biol Plant 36(2):102–107

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15(3):473–497

    Article  CAS  Google Scholar 

  • Nada S, Chennareddy S, Goldman S (2011) Direct shoot bud differentiation and plantlet regeneration from leaf and petiole explants of Begonia tuberhybrida. HortSci 46(5):759–764

    CAS  Google Scholar 

  • Nathiya S, Pradeepa D, Devasena T, Senthil K (2013) Studies on the effect of sucrose, light and hormones on micropropagation and in vitro flowering of Withania somnifera var.jawahar-20. J Anim Plant Sci 23(5):1391–1397

    CAS  Google Scholar 

  • Nayanakantha NMC, Singh BR, Kumar A (2010) Improved culture medium for micropropagation of Aloe vera L. Trop Agric Res Ext 13(4):87–93

    Article  Google Scholar 

  • Ni Y, Tizard IR (2004) Analytical methodology: the gel analysis of Aloe pulp and its derivatives. In: Reynolds T (ed) Aloes: the genus Aloe, 2nd edn. CRC Press, Boca Raton, pp 111–126

    Google Scholar 

  • Okudera Y, Ito M (2009) Production of agarwood fragrant constituent in Aquilaria calli and cell suspension culture. Plant Biotech 26(3):307–315

    Article  CAS  Google Scholar 

  • Park YI, Lee SK (2006) New perspectives on aloe, 1st edn. Springer, New York

    Book  Google Scholar 

  • Peddabonia V, Thamidala C, Karampuri S (2006) In vitro shoot multiplication and plant regeneration in four Capsicum species using thidiazuron. Sci Hortic 107(2):117–122

    Article  Google Scholar 

  • Qi SY, He ML, Lin LD, Zhang CH, Hu LJ, Zhang HZ (2005) Production of 2-(2-phenylethyl) chromones in cell suspension cultures of Aquilaria sinensis. Plant Cell Tissue Organ Cult 83(2):217–221

    Article  CAS  Google Scholar 

  • Qu L, Polashock J, Vorsa N (2000) A high efficient in vitro cranberry regeneration system using leaf explants. HortSci 35(5):948–952

    CAS  Google Scholar 

  • Roy SC, Sarkar A (1991) In vitro regeneration and micropropagation of Aloe vera (L.) Sci Hortic 47(1–2):107–113

    Article  Google Scholar 

  • Sahoo S, Rout GR (2014) Plant regeneration from leaf explants of Aloe barbadensis Mill. and genetic fidelity assessment through DNA markers. Physiol Mol Biol Plants 20(2):235–240

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sajid ZA, Aftab F (2009) Effect of thidiazuron (TDZ) on in vitro micropropagation of Solanum tuberosum L. cvs. desiree and cardinal. Pak J Bot 41(4):1811–1815

    CAS  Google Scholar 

  • Saroha V, Yadav RC, Yadav NR (2005) Remove from marked Records High frequency shoot regeneration in Aloe vera using BAP and TDZ combinations of growth regulators. In: ICAE national symposium on biotechnological interventions for improvement of horticultural crops: issues and strategies, Vellanikkara, Kerala, India, 10–12 January 2005

  • Saroj PL, Dhandar DG, Singh RS (2004) Indian aloe, 1st edn. Central Institute for Arid Horticulture, Bikaner, pp 6–10

    Google Scholar 

  • Sasikumar S, Raveendar S, Premkumar A, Ignacimuthu S, Agastian P (2009) Micropropagation of Baliospermum montanum (Willd.) Muell. Ara.-A threatened medicinal plant. Indian J Biotechnol 8(2):223–226

    CAS  Google Scholar 

  • Sathyagowri S, Seran TH (2011) In vitro plant regeneration of ginger (Zingiber officinale Rosc.) with emphasis on initial culture establishment. Int J Med Aroma Plant 1(3):195–202

    Google Scholar 

  • Sathyagowri S, Seran TH (2013) A note on morphological studies of shoot bud initiation from ginger (Zingiber officinales Rosc) rhizome cultured in vitro conditions. Sudan J Agric Res 22:101–106

    Google Scholar 

  • Singh SK, Syamal MM (2001) A short pre-culture soak in thidiazuron or forchlorfenuron improves axillary shoot proliferation in rose micropropagation. Sci Hortic 91(1–2):169–177

    Article  CAS  Google Scholar 

  • Singh M, Rathore MS, Panwar D, Rathore JS, Dagla HR, Shekhawat NS (2009) Micropropagation of selected genotype of Aloe vera L–an ancient plant for modern industry. J Sustain For 28(8):935–950

    Article  Google Scholar 

  • Steenkamp V, Stewart MJ (2007) Medicinal applications and toxicological activities of aloe products. Pharm Biol 45(5):411–420

    Article  CAS  Google Scholar 

  • Tanabe MJ, Horiuchi K (2006) Aloe barbadensis Miller ex vitro autotrophic culture. J Hawahan Pac Agric 13:55–59

    Google Scholar 

  • Tawfik AA, Mohamed MF (2005) Organogenic response of Salvia officinalis L. to dark preconditioning, thidiazuron and benzyladenine. Plant Growth Regul Soc Am Q 33(4):125–133

    CAS  Google Scholar 

  • Thinesh A, Seran TH (2015) In vitro callogenesis from bud and stem explants of dragon fruit (Hylocereus undatus). Asian J Pharma Sci Tech 5(4):253–256

    Article  Google Scholar 

  • Thomson PDR (2004) Herbal medicines, 3rd edn. Thomson PDR, Montvale

    Google Scholar 

  • Uranbey S (2005) Thidiazuron induced adventitious shoot regeneration in Hyoscyamus niger. Biol Plant 49(3):427–430

    Article  CAS  Google Scholar 

  • Velcheva M, Faltin Z, Vandi A, Eshdat Y, Perl A (2005) Regeneration of Aloe arborescens via organogenesis from young inflorescences. Plant Cell Tissue Organ Cult 83(3):293–301

    Article  Google Scholar 

  • Victório CP, Lage CLS, Sato A (2012) Tissue culture techniques in the proliferation of shoots and roots of Calendula officinalis. Rev Ciênc Agron 43(3):539–545

    Article  Google Scholar 

  • Wang R, He LS, Xia B, Tong JF, Li N, Peng F (2009) A micropropagation system for cloning of hemp (Cannabis sativa L.) by shoot tip culture. Pak J Bot 41(2):603–608

    CAS  Google Scholar 

  • Wu HZ (2000) Tissue culture of Aloe arborescens Mill. Acta Hortic Sin 27(2):151–152

    Google Scholar 

  • Yildirim AB, Turker AU (2009) In vitro adventitious shoot regeneration of the medicinal plant meadowsweet (Filipendula ulmaria (L.) Maxim). In Vitro Cell Dev Biol 45(2):135–144

    Article  Google Scholar 

  • You JH (2001) Advances in the study on tissue culture of Aloe. Chin Wild Plant Resour 20(2):10–11

    Google Scholar 

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Authors and Affiliations

  1. Department of Crop Science, Faculty of Agriculture, Eastern University, Chenkalady, Sri Lanka

    Thayamini H. Seran

  2. Department of Botany, Aligarh Muslim University, Aligarh, UP, India

    Naseem Ahmad

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  1. Thayamini H. Seran
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Correspondence to Thayamini H. Seran .

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  1. Department of Botany, Aligarh Muslim University, Aligarh, UP, India

    Naseem Ahmad

  2. Department of Botany & Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia

    Mohammad Faisal

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Seran, T.H., Ahmad, N. (2018). Shoot Organogenesis of Aloe Plants with Emphasis on TDZ. In: Ahmad, N., Faisal, M. (eds) Thidiazuron: From Urea Derivative to Plant Growth Regulator. Springer, Singapore. https://doi.org/10.1007/978-981-10-8004-3_20

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