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TDZ: Mode of Action, Use and Potential in Agriculture

  • Jaroslav Nisler
Chapter

Abstract

Strong cytokinin effects of thidiazuron (TDZ) in many plant species have been observed since its discovery in the 1970s. Several of these effects, such as cell division stimulatory activity, anti-senescence, anti-stress activity and ethylene production stimulation, have been adopted by agriculturalists and horticulturalists for a wide range of use. TDZ has been shown to promote the growth of various fruits, delay senescence of cut and potted flowers, increase stress tolerance and yield of several crops and cause defoliation of cotton. In this chapter, the mechanisms of how TDZ affects the desired traits are described, and the literature provides evidences reviewed. The information given here should convince everyone that TDZ is not a mysterious substance but that it triggers classical cytokinin responses in plants as successfully as natural cytokinins, no matter whether directly or indirectly. A direct TDZ effect is mediated through the activation of all the cytokinin receptors in plants and their downstream associated signalling pathways. The indirect effect of TDZ is considered to be its ability to inhibit the enzyme cytokinin oxidase/dehydrogenase which degrades cytokinins. This should lead to the elevation of endogenous cytokinin levels; however, it is not possible to distinguish whether the cytokinin effect was the effect of TDZ or the effect of endogenous cytokinins, since both share the same binding site in the proteins and the mechanism of action.

Keywords

Thidiazuron Arabidopsis Cytokinin oxidase/dehydrogenase Horticulture Agriculture Senescence Stress tolerance Defoliation Cotton Cut flower Ethylene 

Notes

Acknowledgements

I would like to thank professor Miroslav Strnad and Lukáš Spíchal for the valuable comments, which contributed to the final version of this chapter. I wish to thank my wife Tereza Nislerová for the English corrections.

References

  1. Abad A, Agulloä C, Cunat AC et al (2004) Preparation and promotion of fruit growth in kiwifruit of fluorinated N-phenyl-N′-1,2,3-thiadiazol-5-yl ureas. J Agric Food Chem 52:4675–4683PubMedCrossRefGoogle Scholar
  2. Abeles FB (1966) Mechanism of action of abscission accelerators. Physiol Plant 20:442–454CrossRefGoogle Scholar
  3. Abeles FB, Dunn LJ, Morgens P et al (1988) Induction of 33-kD and 60-kD peroxidases during ethylene-induced senescence of cucumber cotyledons. Plant Physiol 87:609–615PubMedPubMedCentralCrossRefGoogle Scholar
  4. Arima Y, Oshima K, Shudo K (1995) Evolution of a novel urea-type cytokinin: horticultural uses of forchlorfenuron. Acta Hortic 394:75–84CrossRefGoogle Scholar
  5. Arndt F, Rusch R, Stilfried HV (1976) SN 49537, a new cotton defoliant. Plant Physiol 57:S–99Google Scholar
  6. Bagheri H, Sedaghathour S (2013) Effect of thidiazuron and naphthalene acetic acid (NAA) on the vase life and quality of cut Alestroemeria hybrida. J Ornamental Hort Plants 3:111–116Google Scholar
  7. Balibrea Lara ME, Gonzalez Garcia MC, Fatima T et al (2004) Extracellular invertase is an essential component of cytokinin-mediated delay of senescence. Plant Cell 16:1276–1287PubMedPubMedCentralCrossRefGoogle Scholar
  8. Beckett RP, van Staden J (1992) The effect of thidiazuron on the yield of salinity stressed wheat. Ann Bot 70:47–51CrossRefGoogle Scholar
  9. Bilyeu KD, Cole JL, Laskey JG et al (2001) Molecular and biochemical characterization of a cytokinin oxidase from maize. Plant Physiol 125:378–386PubMedPubMedCentralCrossRefGoogle Scholar
  10. Brandstatter I, Kieber JJ (1998) Two genes with similarity to bacterial response regulators are rapidly and specifically induced by cytokinin in Arabidopsis. Plant Cell 10:1009–1019PubMedPubMedCentralCrossRefGoogle Scholar
  11. Brenner WG, Ramireddy E, Heyl A et al (2012) Gene regulation by cytokinin in Arabidopsis. Front Plant Sci 3:8. https://doi.org/10.3389/fpls.2012.00008 PubMedPubMedCentralCrossRefGoogle Scholar
  12. Brenner WG, Romanov GA, Kollmer I et al (2005) Immediate early and delayed cytokinin response genes of Arabidopsis thaliana identified by genome wide expression profiling reveal novel cytokinin sensitive processes and suggest cytokinin action through transcriptional cascades. Plant Mol Biol 44:314–333Google Scholar
  13. Brown KM (1997) Ethylene and abscission. Physiol Plant 100:567–576CrossRefGoogle Scholar
  14. Brownlee BG, Hall RH, Whitty CD (1975) 3-Methyl-2-butenal: an enzymatic degradation product of the cytokinin, N-6-(delta-2 isopentenyl) adenine. Can J Biochem 53:37–41PubMedCrossRefGoogle Scholar
  15. Bruce MI, Zwar JA (1966) Cytokinin activity of some substituted ureas and thioureas. Proc R Soc Lond Ser B 165:245–265CrossRefGoogle Scholar
  16. Cary AJ, Liu W, Howell SH (1995) Cytokinin action is coupled to ethylene in its effects on the inhibition of root and hypocotyl elongation in Arabidopsis thaliana seedlings. Plant Physiol 107:1075–1082PubMedPubMedCentralCrossRefGoogle Scholar
  17. Cathey GW (1986) Physiology of defoliation in cotton production. In: Mauney JR, Stewart JM (eds) Cotton physiology. The Cotton Foundation, Memphis, pp 143–154Google Scholar
  18. Chamani E, Irving DE, Joyce DC et al (2006) Studies with thidiazuron on the vase life of cut rose flowers. J Appl Hortic 8:42–44Google Scholar
  19. Chang H, Jones ML, Banowetz GM et al (2003) Overproduction of cytokinins in petunia flowers transformed with PSAG12-IPT delays corolla senescence and decreases sensitivity to ethylene. Plant Physiol 132:2174–2183PubMedPubMedCentralCrossRefGoogle Scholar
  20. Chatfield JM, Armstrong DJ (1986) Regulation of cytokinin oxidase activity in callus tissues of Phaseolus vulgaris L. cv Great Northern. Plant Physiol 80:493–499PubMedPubMedCentralCrossRefGoogle Scholar
  21. Chernyaďev II (1994) Effect of 6-benzylaminopurine and thidiazuron on photosynthesis in crop plants. Photosynthetica 30:287–292Google Scholar
  22. Chernyaďev II (2009) The protective action of cytokinins on the photosynthetic machinery and productivity of plants under stress (review). Appl Biochem Microbiol 45:351–362CrossRefGoogle Scholar
  23. Chory J, Reinecke D, Sim S et al (1994) A role for cytokinins in de-etiolation in Arabidopsis (det mutants have an altered response to cytokinins). Plant Physiol 104:339–347Google Scholar
  24. Cutler SR, Rodriguez PL, Finkelstein RR et al (2010) Abscisic acid: emergence of a core signaling network. Annu Rev Plant Biol 61:651–679PubMedCrossRefGoogle Scholar
  25. D’Agostino IB, Deruere J, Kieber JJ (2000) Characterization of the response of the Arabidopsis response regulator gene family to cytokinin. Plant Physiol 124:1706–1717Google Scholar
  26. Debata A, Murty KS (1981) Relation between leaf and panicle senescence in rice. Indian J Exp Biol 19:1183–1184Google Scholar
  27. Dewitte W, Scofield S, Alcasabas AA et al (2007) Arabidopsis CYCD3 D-type cyclins link cell proliferation and endocycles and are rate-limiting for cytokinin responses. PNAS USA 104:14537–14542Google Scholar
  28. Dhiman MR, Guleria MS, Parkash C et al (2015) Effect of different chemical compounds on leaf chlorophyll content and postharvest quality of Lilium. Int J Hort 5:1–6. https://doi.org/10.5376/ijh.2015.05.0018 Google Scholar
  29. Dwivedi SK, Arora A, Singh VP et al (2017) Induction of water deficit tolerance in wheat due to exogenous application of plant growth regulators: membrane stability, water relations and photosynthesis. Photosynthetica. https://doi.org/10.1007/s11099-017-0695-2
  30. El-Beltagy AS, Hall MA (1974) Effect of water stress upon endogenous ethylene levels in Vicia faba. New Phytol 73:47–59CrossRefGoogle Scholar
  31. Elfving DC, Cline RA (1993) Benzyladenine and other chemicals for thinning ‘Empire’ apple trees. J Am Soc Hort Sci 118:593–598Google Scholar
  32. Famiani F, Battistelli A, Moscatello S et al (1999) Thidiazuron affects growth, ripening and quality of Actinidia deliciosa. J Hort Sci Biotech 74:375–380CrossRefGoogle Scholar
  33. Ferrante A, Hunter DA, Hackett WP (2002b) Thidiazuron – a potent inhibitor of leaf senescence in Alstroemeria. Postharvest Biol Technol 25:333–338CrossRefGoogle Scholar
  34. Ferrante A, Hunter D, Hackett W et al (2001) TDZ: a novel tool for preventing leaf yellowing in Alstroemeria flowers. Hortic Sci 36.: Poster:599Google Scholar
  35. Ferrante A, Mensuali-Sodi A, Serra G (2009) Effect of thidiazuron and gibberellic acid on leaf yellowing of cut stock flowers. Cent Eur J Biol 4:61–468Google Scholar
  36. Ferrante A, Mensuali-Sodi A, Serra G et al (2002a) Effects of ethylene and cytokinins on vase life of cut Eucalyptus parvifolia Cambage branches. Plant Growth Regul 38:119–125CrossRefGoogle Scholar
  37. Ferrante A, Mensuali-Sodi A, Serra G et al (2003) Treatment with thidiazuron for preventing leaf yellowing in cut tulips, and chrysanthemum. Acta Hortic 624:357–363CrossRefGoogle Scholar
  38. Ferrante A, Mensuali-sodi A, Tognoni F et al (2005) Postharvest studies on leaf yellowing of chrysanthemum cut flowers. Adv Hortic Sci 19:81–82Google Scholar
  39. Ferrante A, Trivellini A, Mensuali-Sodi A (2012) Interaction of 1-methylcyclopropene and thidiazuron on cut stock flowers vase life. Open Hort J 5:1–5CrossRefGoogle Scholar
  40. Ferrante A, Trivellini A, Serra A (2011) Benzyladenine and thidiazuron postharvest treatments for preserving cut lily flowers. Acta Hortic 900:301–307CrossRefGoogle Scholar
  41. Ferrante A, Vernieri P, Serra G et al (2004) Changes in abscisic acid during leaf yellowing of cut stock flowers. Plant Growth Regul 43:127–134CrossRefGoogle Scholar
  42. Flores S, Tobin EM (1988) Cytokinin modulation of LHCP mRNA levels: the involvement of post-transcriptional regulation. Plant Mol Biol 11:409–415PubMedCrossRefGoogle Scholar
  43. Forshey CG (1987) A review of chemical fruit thinning. Proc NE Fruit Meet 93:68–73Google Scholar
  44. Galuszka P, Popelková H, Werner T et al (2007) Biochemical characterization and histochemical localization of cytokinin oxidases/dehydrogenases from Arabidopsis thaliana expressed in Nicotiana tabaccum L. J Plant Growth Regul 26:255–267CrossRefGoogle Scholar
  45. Gan S, Amasino RM (1995) Inhibition of leaf senescence by autoregulated production of cytokinin. Science 270:1986–1988PubMedCrossRefGoogle Scholar
  46. Gan S, Amasino RM (1997) Making sense of senescence (molecular genetic regulation and manipulation of leaf senescence). Plant Physiol 113:313–319PubMedPubMedCentralCrossRefGoogle Scholar
  47. Grbić V, Bleecker AB (1995) Ethylene regulates the timing of leaf senescence in Arabidopsis. Plant J 8:595–602Google Scholar
  48. Greenboim-Wainberg Y, Maymon I, Borochov R et al (2004) Cross talk between gibberellin and cytokinin: the Arabidopsis GA response inhibitor SPINDLY plays a positive role in cytokinin signaling. Plant Cell 17:92–102Google Scholar
  49. Greene DW (1995) Thidiazuron effects on fruit set, fruit quality, and return bloom of apples. Hortic Sci 30:1238–1240Google Scholar
  50. Grossmann K (1991) Induction of leaf abscission in cotton is a common effect of urea- and adenine-type cytokinins. Plant Physiol 95:234–237PubMedPubMedCentralCrossRefGoogle Scholar
  51. Hare PD, Van Staden J (1994) Inhibitory effect of thidiazuron on the activity of cytokinin oxidase isolated from soybean callus. Plant Cell Physiol 35:1121–1125CrossRefGoogle Scholar
  52. Hatami M, Hatamzadeh A, Ghasemnezhad M et al (2013) Antioxidant enzymatic protection during Pelargonium plant leaf senescence is mediated by thidazuron. Trakia J Sci 11:152–157Google Scholar
  53. Hatamzadeh A, Rezvanypour S, Asil MH (2012) Postharvest life of Alstroemeria cut flowers is extended by thidiazuron and benzyladenine. South West J Hort Biol Env 3:41–53Google Scholar
  54. Hauska G, Trebst A, Koetter C et al (1975) 1,2,3-Thiadiazolyl-phenyl-ureas, new inhibitors of photosynthetic and respiratory energy conservation. Z Naturforsch C J Biosci 30:505–510Google Scholar
  55. Hensel LL, Grbić V, Baumgarten DA et al (1993) Developmental and age-related processes that influence the longevity and senescence of photosynthetic tissues in Arabidopsis. Plant Cell 5:553–564Google Scholar
  56. Hodgson RH, Snyder RH (1988) Thidiazuron effects on Malvaceae; corn, (Zea mays); and soybean, (Glycine max). Weed Technol 2:342–349CrossRefGoogle Scholar
  57. Hothorn M, Dabi T, Chory J (2011) Structural basis for cytokinin recognition by Arabidopsis thaliana histidine kinase 4. Nat Chem Biol 7:766–768PubMedPubMedCentralCrossRefGoogle Scholar
  58. Houba-Hérin N, Pethe C, d’Alayer J et al (1999) Cytokinin oxidase from Zea mays: purification, cDNA cloning and expression in moss protoplasts. Plant J 17:615–626PubMedCrossRefGoogle Scholar
  59. Hunter DA, Yoo SD, Butcher SM et al (1999) Expression of 1-aminocyclopropane-1-carboxylate oxidase during leaf ontogeny in white clover. Plant Physiol 120:131–142PubMedPubMedCentralCrossRefGoogle Scholar
  60. Ichimura K, Hiraya T (1999) Effect of silver thiosulfate complex (STS) in combination with sucrose on the vase life of cut sweet pea flowers. J Jpn Soc Hort Sci 68:23–27CrossRefGoogle Scholar
  61. Inoue T, Higuchi M, Hashimoto Y et al (2001) Identification of CRE1 as a cytokinin receptor from Arabidopsis. Nature 409:1060–1063Google Scholar
  62. Itai A, Tanabe K, Tamura F et al (1995) Synthetic cytokinins control persimmon fruit shape, size and quality. J Hort Sci 70:867–873Google Scholar
  63. Jiang CZ, Wu L, Macnish AJ et al (2009) Thidiazuron, a non-metabolized cytokinin, shows promise in extending the life of potted plants. Acta Hortic 847:59–66CrossRefGoogle Scholar
  64. Jing HC, Schippers JH, Hille J et al (2005) Ethylene-induced leaf senescence depends on age-related changes and OLD genes in Arabidopsis. J Exp Bot 56:2915–2923Google Scholar
  65. Jo YS, Cho HS, Park MY et al (2003) Comparison of CPPU effects on fruit development in several actinidia species. Acta Hortic 610:539–543CrossRefGoogle Scholar
  66. Jordi W, Schapendonk A, Davelaar E et al (2000) Increased cytokinin levels in transgenic PSAG12-IPT tobacco plants have large direct and indirect effects on leaf senescence, photosynthesis and N partitioning. Plant Cell Environ 23:279–289CrossRefGoogle Scholar
  67. Jordi W, Stoopen GM, Kelepouris K et al (1995) Gibberellin-induced delay of leaf senescence of Alstroemeria cut flowering stems is not caused by an increase in the endogenous cytokinin content. J Plant Growth Regul 14:121–127CrossRefGoogle Scholar
  68. Kaur P, Singh K (2015) Influence of growth regulators on physiology and senescence of cut stems of Chrysanthemum (Chrysanthemum morifolium Ramat) Var. Thai Ching Queen IJAPRR 2:31–41Google Scholar
  69. Kaviani M, Mortazavi SN (2013) Effect of nitric oxide and thidiazuron on Lilium cut flowers during postharvest. Int J Agron Plant Prod 4:664–669Google Scholar
  70. Kefford NP, Bruce MI, Zwar JA (1973) Retardation of leaf senescence by urea cytokinins in Raphanus sativus. Phytochemistry 12:995–1003CrossRefGoogle Scholar
  71. Kiba T, Yamada H, Sato S et al (2003) The type-A response regulator, ARR15, acts as a negative regulator in the cytokinin-mediated signal transduction in Arabidopsis thaliana. Plant Cell Physiol 44:868–874PubMedCrossRefGoogle Scholar
  72. Kim HJ, Ryu H, Hong SH et al (2006) Cytokinin-mediated control of leaf longevity by AHK3 through phosphorylation of ARR2 in Arabidopsis. PNAS USA 103:814–819PubMedPubMedCentralCrossRefGoogle Scholar
  73. Kopečný D, Briozzo P, Popelková H et al (2010) Phenyl- and benzylurea cytokinins as competitive inhibitors of cytokinin oxidase/dehydrogenase: a structural study. Biochimie 92:1052–1062PubMedCrossRefGoogle Scholar
  74. Koprna R, De Diego N, Dundálková L et al (2016) Use of cytokinins as agrochemicals. Bioorg Med Chem 24:484–492PubMedCrossRefGoogle Scholar
  75. Kraepiel Y, Miginiac E (1997) Photomorphogenesis and phytohormones. Plant Cell Environ 20:807–812CrossRefGoogle Scholar
  76. Kusnetsov VV, Oelmüller R, Sarwat MI et al (1994) Cytokinins, abscisic acid and light affect accumulation of chloroplast proteins in Lupinus luteus cotyledons without notable effect on steady-state mRNA levels. Planta 194:318–327CrossRefGoogle Scholar
  77. Laureys F, Dewitte W, Witters E et al (1998) Zeatin is indispensable for the G2-M transition in tobacco BY-2 cells. FEBS Lett 426:29–32PubMedCrossRefGoogle Scholar
  78. Leibfried A, To JPC, Stehling S et al (2005) WUSCHEL controls meristem size by direct transcriptional regulation of cytokinin inducible response regulators. Nature 438:1172–1175PubMedCrossRefGoogle Scholar
  79. Leonard RT, Nell TA (2004) Short-term pulsing improves postharvest leaf quality of cut oriental lilies. Hort Tech 14:405–411Google Scholar
  80. Leshem YY, Wills RBH (1998) Harnessing senescence delaying gases nitric oxide and nitrous oxide: a navel approach to postharvest control of fresh horticultural produce. Biol Plant 41:1–100CrossRefGoogle Scholar
  81. Lomin SN, Krivosheev DM, Steklov MY et al (2015) Plant membrane assays with cytokinin receptors underpin the unique role of free cytokinin bases as biologically active ligands. J Exp Bot 66:1851–1863PubMedPubMedCentralCrossRefGoogle Scholar
  82. Macnish AJ, Jiang CZ, Reid MS (2010) Treatment with thidiazuron improves opening and vase life of iris flowers. Postharvest Biol Technol 56:77–84CrossRefGoogle Scholar
  83. Mähönen AP, Bonke M, Kaupinnen L et al (2000) A novel two-component hybrid molecule regulates vascular morphogenesis of the Arabidopsis root. Genes Dev 14:2938–2943PubMedPubMedCentralCrossRefGoogle Scholar
  84. Malik MN, Din S (1997) Efficacy of thidiazuron defoliant in cotton cultivars varying in maturity. Pak Cottons 41:36–42Google Scholar
  85. Malik MN, Din S, Makhdum MI (1991) Accelerated boll dehiscence with thidiazuron. Trop Agric 68:149–150Google Scholar
  86. Malik MN, Din S, Makhdum MI et al (2002) Use of thidiazuron as harvest-aid in early and late planted cotton. Int J Agric Biol 4:71–73Google Scholar
  87. Masferrer A, Arro M, Manzano D et al (2002) Overexpression of Arabidopsis thaliana farnesyl diphosphate synthase (FPS1S) in transgenic Arabidopsis induces a cell death/senescence-like response and reduced cytokinin levels. Plant J 30:123–132Google Scholar
  88. Mayak S, Dilley DR (1976) Regulation of senescence in carnation (Dianthus caryophylus). Plant Physiol 58:663–665PubMedPubMedCentralCrossRefGoogle Scholar
  89. Mayak S, Halevy AH (1972) Interrelationships of ethylene and abscisic acid in the control of rose petal senescence. Plant Physiol 50:341–346PubMedPubMedCentralCrossRefGoogle Scholar
  90. Mik V, Szüčová L, Šmehilová M et al (2011) N9-substituted derivatives of kinetin: effective anti-senescence agents. Phytochemistry 72:821–831PubMedCrossRefGoogle Scholar
  91. Miller CO, Skoog F, Von Saltza MH et al (1955) Kinetin, a cell division factor from deoxyribonucleic acid. J Amer Chem Soc 77:1392CrossRefGoogle Scholar
  92. Mok MC, Martin RC, Dobrev PI et al (2005) Topolins and hydroxylated thidiazuron derivatives are substrates of cytokinin o-glucosyltransferase with position specificity related to receptor recognition. Plant Physiol 137:1057–1066PubMedPubMedCentralCrossRefGoogle Scholar
  93. Mok MC, Mok DWS (1985) The metabolism of [14C] thidiazuron in callus tissues of Phaseolus lunatus. Physiol Plant 65:427–432CrossRefGoogle Scholar
  94. Mok MC, Mok DWS, Amstrong DJ et al (1982) Cytokinin activity of N-phenyl-N-1,2,3-thidiazol-5-ylurea (thidiazuron). Phytochemistry 21:1509–1511CrossRefGoogle Scholar
  95. Morris RO, Bilyeu KD, Laskey JG et al (1999) Isolation of a gene encoding a glycosylated cytokinin oxidase from maize. Biochem Biophys Res Commun 255:328–333PubMedCrossRefGoogle Scholar
  96. Mortazavi SN, Talebi SF, Naderi RA et al (2011) Regulation of ethylene biosynthesis by nitric oxide and thidiazuron during postharvest of rose. J Med Plant Res 5:5177–5183Google Scholar
  97. Mothes K, Engelbrecht L (1963) On the activity of a kinetin-like root factor. Life Sci 11:852–857CrossRefGoogle Scholar
  98. Murch SJ, Saxena PK (2001) Molecular fate of thidiazuron and its effects on auxin transport in hypocotyls tissues of Pelargonium x hortorum Bailey. Plant Growth Regul 35:269–275CrossRefGoogle Scholar
  99. Mutui TM, Emongor VN, Hutchinson MJ (2003) Effect of benzyladenine on the vase life and keeping quality of Alstroemeria cut flowers. J Agric Sci Technol 5:91–105Google Scholar
  100. Mutui TM, Mibus H, Serek M (2005) Effects of thidiazuron, ethylene, abscisic acid and dark storage on leaf yellowing and rooting of Pelargonium cuttings. J Hortic Sci Biotechnol 80:543–550CrossRefGoogle Scholar
  101. Mutui TM, Mibus H, Serek M (2007) Influence of thidiazuron, ethylene, abscisic acid and dark storage on the expression levels of ethylene receptors (ETR) and ACC synthase (ACS) genes in Pelargonium. Plant Growth Regul 53:87–96CrossRefGoogle Scholar
  102. Nagar S, Arora A, Singh VP et al (2015) Effect of cytokinin analogues on cytokinin metabolism and stress responsive genes under osmotic stress in wheat. Bioscan 10:67–72Google Scholar
  103. Nisler J, Kopečný D, Končitíková R et al (2016) Novel thidiazuron-derived inhibitors of cytokinin oxidase/dehydrogenase. Plant Mol Biol 92:235–248PubMedCrossRefGoogle Scholar
  104. Pavlista AD (2003) Thidiazuron, a cytokinin-like compound, enhances fungicidal activity against early blight in potato. Acta Hortic 619:145–152CrossRefGoogle Scholar
  105. Petri JL, Schuck E, Leite GB (2001) Effects of thidiazuron (tdz) on fruiting of temperate tree fruits. Rev Bras Frutic 23:513–517CrossRefGoogle Scholar
  106. Rashotte AM, Mason MG, Hutchison CE et al (2006) A subset of Arabidopsis AP2 transcription factors mediates cytokinin responses in concert with a two-component pathway. PNAS USA 103:11081–11085Google Scholar
  107. Redig P, Shaul O, Inze D et al (1996) Levels of endogenous cytokinins, indole-3-acetic acid and abscisic acid during the cell cycle of synchronized tobacco BY-2 cells. FEBS Lett 391:175–180PubMedCrossRefGoogle Scholar
  108. Reid MS (1995) Ethylene in plant growth, development, and senescence. In: Davis PJ (ed) Plant hormones. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0473-9_23 Google Scholar
  109. Reynolds AG, Wardle DA, Zurowski C et al (1992) Phenylureas CPPU and thidiazuron affect yield components, fruit composition, and storage potential of four seedless grape selections. J Am Soc Hort Sci 117:85–89Google Scholar
  110. Richmond AE, Lang A (1957) Effect of kinetin on protein content and survival of detached Xanthium leaves. Sci NY 125:650–651CrossRefGoogle Scholar
  111. Riefler M, Novak O, Strnad M et al (2006) Arabidopsis cytokinin receptor mutants reveal functions in shoot growth, leaf senescence, seed size, germination, root development, and cytokinin metabolism. Plant Cell 18:40–54PubMedPubMedCentralCrossRefGoogle Scholar
  112. Riou-Khamlichi C, Huntley R, Jacqmard A et al (1999) Cytokinin activation of Arabidopsis cell division through a D-type cyclin. Science 283:1541–1544Google Scholar
  113. Rivero RM, Kojima M, Gepstein A et al (2007) Delayed leaf senescence induces extreme drought tolerance in a flowering plant. PNAS USA 104:19631–19636PubMedPubMedCentralCrossRefGoogle Scholar
  114. Romanov GA, Lomin SN, Schmülling T (2006) Biochemical characteristics and ligand-binding properties of Arabidopsis cytokinin receptor AHK3 compared to CRE1/AHK4 as revealed by a direct binding assay. J Exp Bot 57:4051–4058Google Scholar
  115. Sakakibara H (2003) Nitrate-specific and cytokinin-mediated nitrogen signaling pathways in plants. J Plant Res 116:253–257PubMedCrossRefGoogle Scholar
  116. Sankhla N, Mackay WA, Davis TD (2003) Reduction of flower abscission and leaf senescence in cut Phlox inflorescences by thidiazuron. Acta Hort 628:837–841CrossRefGoogle Scholar
  117. Sankhla N, Mackay WA, Davis TD (2005) Effect of thidiazuron on senescence of flowers in cut inflorescences of Lupinus densiflorus benth. Acta Hortic 669:239–244CrossRefGoogle Scholar
  118. Schaller GE, Kieber JJ, Shiu S (2008) Two-component signalling elements and histidyl-aspartyl phosphorelays. In: Somerville C, Meyerowitz EM (eds) The Arabidopsis book. American Society of Plant Biologists, Rockville, pp 1–12Google Scholar
  119. Schaller GE, Street IH, Kieber JJ (2014) Cytokinin and the cell cycle. Curr Opin Plant Biol 21:7–15PubMedCrossRefGoogle Scholar
  120. Schippers JHM, Breeze E, Buchanan-Wollaston V (2008). A role for cytokinin in the onset of leaf senescence by ethylene in Arabidopsis. Molecular aspects of ageing and the onset of leaf senescence. Schippers JHMn.d. s.n. 164 p. Doctoral thesisGoogle Scholar
  121. Schulz H, Arndt F (1973) 1,2,3-Thiadiazole plant growth retardants. From Ger. Offen.DE 2214632 A1 19731004Google Scholar
  122. Scofield S, Dewitte W, Nieuwland J et al (2013) The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity. Plant J 75:53–66PubMedCrossRefGoogle Scholar
  123. Singh S, Letham DS, Palni LMS (1992) Cytokinin biochemistry in relation to leaf senescence. 7. Endogenous cytokinin levels and exogenous applications of cytokinins in relation to sequential leaf senescence of tobacco. Physiol Plant 86:388–397CrossRefGoogle Scholar
  124. Spíchal L, Rakova NY, Riefler M et al (2004) Two cytokinin receptors of Arabidopsis thaliana, CRE1/AHK4 and AHK3, differ in their ligand specificity in a bacterial assay. Plant Cell Physiol 45:1299–1305PubMedCrossRefGoogle Scholar
  125. Stern R, Shargal A, Flaishman M (2003) Thidiazuron increases fruit size of ‘Spadona’ and ‘Coscia’ pear (Pyrus communis L.) J Hortic Sci Biotechnol 78:51–55CrossRefGoogle Scholar
  126. Stolz A, Riefler M, Lomin SN et al (2011) The specificity of cytokinin signalling in Arabidopsis thaliana is mediated by differing ligand affinities and expression profiles of the receptors. Plant J 67:157–168PubMedCrossRefGoogle Scholar
  127. Suttle JC (1983) Effect of the defoliant thidiazuron on ethylene production. Plant Physiol 72:S-121Google Scholar
  128. Suttle JC (1984) Effect of the defoliant thidiazuron on ethylene evolution from mung bean hypocotyl segments. Plant Physiol 75:902–907PubMedPubMedCentralCrossRefGoogle Scholar
  129. Suttle JC (1985) Involvement of ethylene in the action of the cotton defoliant thidiazuron. Plant Physiol 78:272–276PubMedPubMedCentralCrossRefGoogle Scholar
  130. Suttle JC (1986) Cytokinin-induced ethylene biosynthesis in non senescing cotton leaves. Plant Physiol 82:930–935PubMedPubMedCentralCrossRefGoogle Scholar
  131. Suttle JC, Hultstrand JF (1991) Ethylene-induced leaf abscission in cotton seedlings. The physiological bases for age-dependent differences in sensitivity. Plant Physiol 95:29–33PubMedPubMedCentralCrossRefGoogle Scholar
  132. Suzuki T, Miwa K, Ishikawa K et al (2001) The Arabidopsis sensor His-kinase, AHK4, can respond to cytokinins. Plant Cell Physiol 42:107–113Google Scholar
  133. Talebi SF, Mortazavi SN, Naderi RA et al (2013) Role of nitric oxide and Thidiazuron on changes of pigments during postharvest in Rosa (Cv. ‘Sensiro’). Int J Agron Plant Prod 4:121–126Google Scholar
  134. Taniguchi M, Kiba T, Sakakidara H et al (1998) Expression of Arabidopsis response regulator homologues is induced by cytokinins and nitrate. FEBS Lett 429:259–262Google Scholar
  135. Thomas H, Howarth CJ (2000) Five ways to stay green. J Exp Bot 51:329–337PubMedCrossRefGoogle Scholar
  136. Tirtashi ZB, Hashemabadi D, Kaviani B et al (2014) Effect of thidiazuron and salicylic acid on the vase life and quality of Alstroemeria (Alstroemeria hybrida L .cv. ‘Modena’) cut flower. J Ornamental Plants 4:163–168Google Scholar
  137. To JPC, Deruere J, Maxwell BB et al (2007) Cytokinin regulates type-A Arabidopsis response regulator activity and protein stability via two-component phosphorelay. Plant Cell 19:3901–3914Google Scholar
  138. To JPC, Haberer G, Ferreira FJ et al (2004) Type-A Arabidopsis response regulators are partially redundant negative regulators of cytokinin signalling. Plant Cell 16:658–671Google Scholar
  139. Ueguchi C, Sato S, Kato T et al (2001) The AHK4 gene involved in the cytokinin-signalling pathway as a direct receptor molecule in Arabidopsis thaliana. Plant Cell Physiol 42:751–755PubMedCrossRefGoogle Scholar
  140. Uthairatanakij A, Jeenbuntug J, Buanong M et al (2007) Effect of thidiazuron pulsing on physiological changes of cut tuberose flower (Polianthes tuberosa L.) Acta Hortic 755:477–480CrossRefGoogle Scholar
  141. Van Staden J (1973) Changes in endogenous cytokinin levels during abscission and senescence of streptocarpus leaves. J Exp Bot 24:667–671CrossRefGoogle Scholar
  142. Vogel JP, Woeste KE, Theologis A et al (1998) Recessive and dominant mutations in the ethylene biosynthetic gene ACS5 of Arabidopsis confer cytokinin insensitivity and ethylene overproduction, respectively. PNAS USA 95:4766–4771Google Scholar
  143. Weaver LM, Gan S, Quirino B et al (1998) A comparison of the expression patterns of several senescence-associated genes in response to stress and hormone treatment. Plant Mol Biol 37:455–469PubMedCrossRefGoogle Scholar
  144. Werner T, Motyka V, Laucou V et al (2003) Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity. Plant Cell 15:2532–2550Google Scholar
  145. Whitty CD, Hall RH (1974) A cytokinin oxidase in Zea mays. Can J Biochem 52:789–799PubMedCrossRefGoogle Scholar
  146. Wingler A, von Schaewen A, Leegood RC et al (1998) Regulation of leaf senescence by cytokinin, sugars, and light. Effect on NADH-dependent hydroxypyruvate reductase. Plant Physiol 116:329–335PubMedCentralCrossRefGoogle Scholar
  147. Woeste KE, Vogel JP, Kieber JJ (1999) Factors regulating ethylene biosynthesis in etiolated Arabidopsis thaliana seedlings. Physiol Plant 105:478–484CrossRefGoogle Scholar
  148. Yamada H, Suzuki T, Terada K et al (2001) The Arabidopsis AHK4 histidine kinase is a cytokinin-binding receptor that transduces cytokinin signals across the membrane. Plant Cell Physiol 42:1017–1023PubMedCrossRefGoogle Scholar
  149. Yang SF, Hoffman NE (1984) Ethylene biosynthesis and its regulation in higher plants. Annu Rev Plant Physiol 35:155–189CrossRefGoogle Scholar
  150. Yang YZ, Lin DC, Guo ZY (1992) Promotion of fruit development in cucumber (Cucumis sativus) by thidiazuron. Sci Hortic 50:47–51CrossRefGoogle Scholar
  151. Yaronskaya E, Vershilovskaya I, Poers Y et al (2006) Cytokinin effects on tetrapyrrole biosynthesis and photosynthetic activity in barley seedlings. Planta 224:700–709PubMedCrossRefGoogle Scholar
  152. Yip WK, Yang SF (1986) Effect of thidiazuron, a cytokinin active urea derivative, in cytokinin-dependent ethylene production systems. Plant Physiol 80:515–519PubMedPubMedCentralCrossRefGoogle Scholar
  153. Yoon TM, Richter H (1990) Seasonal changes in stomatal responses of sweet cherry and plum to water status in detached leaves. Physiol Plant 80:520–526CrossRefGoogle Scholar
  154. Yu Y, Yang SF, Corse J et al (1981) Structures of cytokinins influence synergistic production of ethylene. Phytochemistry 20:1191–1195CrossRefGoogle Scholar

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© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Laboratory of Growth Regulators, Centre of the Region Hana for Biotechnological and Agricultural ResearchInstitute of Experimental Botany, AS CR & Palacký UniversityOlomoucCzech Republic

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