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Factors Affecting Thidiazuron-Induced Direct Somatic Embryogenesis of Phalaenopsis aphrodite

  • Wee-Peng Gow
  • Hsiao-Hang Chung
  • Jen-Tsung Chen
  • Wei-Chin Chang
Chapter

Abstract

The effects of NaH2PO4, sucrose, activated charcoal, polyvinylpyrrolidone (PVP), and strength of MS medium were studied to optimize thidiazuron (TDZ)-induced direct somatic embryogenesis from leaf explants of Phalaenopsis aphrodite subsp. formosana. The results showed that full- and quarter-strength macroelements of MS medium were not suitable for direct embryo induction from leaf explants. Thus, a half-strength macroelement and full-strength microelements of MS nutrients plus full-strength of MS vitamins, 170 mg l−1 NaH2PO4, 1 g l−1 peptone, 3 mg l−1 TDZ, and 20 g l−1 sucrose are proposed as a suitably modified medium. In addition, PVP at 0.25 g l−1 significantly promoted direct embryogenesis on the cut ends of the explants, but activated charcoal at 0.5–1 g l−1 was inhibitory.

Keywords

Activated charcoal Direct somatic embryogenesis Embryogenic competence Polyvinylpyrrolidone 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Wee-Peng Gow
    • 1
  • Hsiao-Hang Chung
    • 2
  • Jen-Tsung Chen
    • 3
  • Wei-Chin Chang
    • 1
  1. 1.Institute of Plant and Microbial Biology, Academia SinicaTaipeiRepublic of China
  2. 2.Department of HorticultureNational Ilan UniversityYilan CityRepublic of China
  3. 3.Department of Life SciencesNational University of KaohsiungKaohsiungRepublic of China

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