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Factors Affecting Thidiazuron-Induced Direct Somatic Embryogenesis of Phalaenopsis aphrodite

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Thidiazuron: From Urea Derivative to Plant Growth Regulator

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Abstract

The effects of NaH2PO4, sucrose, activated charcoal, polyvinylpyrrolidone (PVP), and strength of MS medium were studied to optimize thidiazuron (TDZ)-induced direct somatic embryogenesis from leaf explants of Phalaenopsis aphrodite subsp. formosana. The results showed that full- and quarter-strength macroelements of MS medium were not suitable for direct embryo induction from leaf explants. Thus, a half-strength macroelement and full-strength microelements of MS nutrients plus full-strength of MS vitamins, 170 mg l−1 NaH2PO4, 1 g l−1 peptone, 3 mg l−1 TDZ, and 20 g l−1 sucrose are proposed as a suitably modified medium. In addition, PVP at 0.25 g l−1 significantly promoted direct embryogenesis on the cut ends of the explants, but activated charcoal at 0.5–1 g l−1 was inhibitory.

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Authors and Affiliations

  1. Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China

    Wee-Peng Gow & Wei-Chin Chang

  2. Department of Horticulture, National Ilan University, Yilan City, Taiwan, Republic of China

    Hsiao-Hang Chung

  3. Department of Life Sciences, National University of Kaohsiung, Kaohsiung, Taiwan, Republic of China

    Jen-Tsung Chen

Authors
  1. Wee-Peng Gow
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  2. Hsiao-Hang Chung
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  3. Jen-Tsung Chen
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  4. Wei-Chin Chang
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Corresponding author

Correspondence to Jen-Tsung Chen .

Editor information

Editors and Affiliations

  1. Department of Botany, Aligarh Muslim University, Aligarh, UP, India

    Naseem Ahmad

  2. Department of Botany & Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia

    Mohammad Faisal

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Gow, WP., Chung, HH., Chen, JT., Chang, WC. (2018). Factors Affecting Thidiazuron-Induced Direct Somatic Embryogenesis of Phalaenopsis aphrodite . In: Ahmad, N., Faisal, M. (eds) Thidiazuron: From Urea Derivative to Plant Growth Regulator. Springer, Singapore. https://doi.org/10.1007/978-981-10-8004-3_16

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