The Applications of TDZ in Medicinal Plant Tissue Culture

Chapter

Abstract

Thidiazuron (TDZ; N-phenyl-1, 2, 3-thidiazole-5ylurea) is a cytokinin-like compound routinely used for in vitro culture studies including shoot proliferation and regeneration in various plants. Recently, there is an increased use of TDZ in in vitro propagation of plants including medicinal and horticultural crops. TDZ was found to be more effective in micropropagation, multiple shoot induction, somatic embryogenesis, callus induction, and shoot organogenesis as compared to other cytokinins. In some systems the synergistic effect of TDZ with other cytokinin/auxin was found more effective than using alone. The optimum concentration of TDZ may vary based on the plant species, explants, and duration of exposure. In this review we have described the recent trends in the use of TDZ for micropropagation of some medicinal plants. The medicinal plants described in this chapter include Picrorhiza kurroa, Aronia melanocarpa, Scutellaria ocmulgee, Salvia miltiorrhiza, Aphyllorchis montana, Gentiana decumbens, Aconitum balfourii, Pelargonium sidoides, Withania somnifera, Curculigo latifolia, Hedychium coronarium, Mentha arvensis, Clitoria ternatea, Saussurea involucrata, Jatropha curcas, Medicago sativa, Crocus sativus, Zingiber officinale, Cannabis sativa, Arnebia euchroma, and Azadirachta indica.

Keywords

Thidiazuron Medicinal plants Plant tissue culture Multiple shoot formation 

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© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Department of Plant Science, Riverside Transit CampusCentral University of KeralaKasaragodIndia

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