The Applications of TDZ in Medicinal Plant Tissue Culture

  • A. V. Deepa
  • M. Anju
  • T. Dennis Thomas


Thidiazuron (TDZ; N-phenyl-1, 2, 3-thidiazole-5ylurea) is a cytokinin-like compound routinely used for in vitro culture studies including shoot proliferation and regeneration in various plants. Recently, there is an increased use of TDZ in in vitro propagation of plants including medicinal and horticultural crops. TDZ was found to be more effective in micropropagation, multiple shoot induction, somatic embryogenesis, callus induction, and shoot organogenesis as compared to other cytokinins. In some systems the synergistic effect of TDZ with other cytokinin/auxin was found more effective than using alone. The optimum concentration of TDZ may vary based on the plant species, explants, and duration of exposure. In this review we have described the recent trends in the use of TDZ for micropropagation of some medicinal plants. The medicinal plants described in this chapter include Picrorhiza kurroa, Aronia melanocarpa, Scutellaria ocmulgee, Salvia miltiorrhiza, Aphyllorchis montana, Gentiana decumbens, Aconitum balfourii, Pelargonium sidoides, Withania somnifera, Curculigo latifolia, Hedychium coronarium, Mentha arvensis, Clitoria ternatea, Saussurea involucrata, Jatropha curcas, Medicago sativa, Crocus sativus, Zingiber officinale, Cannabis sativa, Arnebia euchroma, and Azadirachta indica.


Thidiazuron Medicinal plants Plant tissue culture Multiple shoot formation 


  1. Agbogidi OM, Akparobi SO, Eruotor PG (2013) Health and environmental benefits of Jatropha curcas Linn. Unique Res J Agric Sci 1:76–79Google Scholar
  2. Ai CB, Li LN (1988) Stereo structure of salvianolic acid B and isolation of salvianolic acid C from Salvia miltiorrhiza. J Nat Prod 51:145–149CrossRefGoogle Scholar
  3. Aishwariya V, Ramrao RK, Kokiladevi E, Arul L, Sudhakar D, Kumar KK, Balasubramanian P (2015) Impact of TDZ (Thidiazuron) pulse treatment in single and multiple shoot formation in calli of Jatropha curcas L. Int JAdv Res 3:879–884Google Scholar
  4. Akula C, Akula A, Drew R (2003) Somatic embryogenesis in clonal neem, Azadirachta indica A. Juss. and analysis for in vitro azadirachtin production. In Vitro Cell Dev Biol-Plant 39:304–310CrossRefGoogle Scholar
  5. Al-Amin ZM, Thomson M, Al-Qattan KK, Peltonen-Shalaby R, Ali M (2006) Anti-diabetic and hypolipidaemic properties of ginger (Zingiber officinale) in streptozotocin-induced diabetic rats. Br J Nutr 96:660–666CrossRefPubMedGoogle Scholar
  6. Ali BH, Blunden G, Tanira MO, Nemmar A (2008) Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): a review of recent research. Food Chem Toxicol 46:409–420CrossRefPubMedGoogle Scholar
  7. Ali EM, Almagboul AZ, Khogali SM, Gergeir UM (2012) Antimicrobial activity of Cannabis sativa L. Chin Med 3:61–64CrossRefGoogle Scholar
  8. Ansari RA, Aswal BS, Chander R, Dhawan BN, Garg NK, Kapoor NK, Kulshreshtha DK, Mehdi H, Mehrotra BN, Patnaik GK (1988) Hepatoprotective activity of kutkin-the iridoid glycoside mixture of Picrorhiza kurroa. Ind J Med Res 87:401–404Google Scholar
  9. Ashkani-Esfahani S, Imanieh MH, Khoshneviszadeh M, Meshksar A, Noorafshan A, Geramizadeh B, Ebrahimi S, Handjani F, Tanideh N (2012) The healing effect of Arnebia euchroma in second degree burn wounds in rat as an animal model. Iran Red Crescent Med J 14:70–74PubMedPubMedCentralGoogle Scholar
  10. Babaei N, Psyquay Abdullah NA, Saleh G, Lee Abdullah T (2014) An efficient in vitro plantlet regeneration from shoot tip cultures of Curculigo latifolia, a medicinal plant. Sci World J2014:1–9Google Scholar
  11. Barre A, Van Damme EJ, Peumans WJ, Rougé P (1997) Curculin, a sweet-tasting and taste-modifying protein, is a non-functional mannose-binding lectin. Plant Mol Biol 33:691–698CrossRefPubMedGoogle Scholar
  12. Bhargava V (2011) Medicinal uses and pharmacological properties of Crocus sativus Linn (Saffron). Int J Pharm Pharma Sci 3:22–26Google Scholar
  13. Casanova E, Valdés AE, Fernández B, Moysset L, Trillas MI (2004) Levels and immunolocalization of endogenous cytokinins in thidiazuron-induced shoot organogenesis in carnation. J Plant Physiol 161:95–104CrossRefPubMedGoogle Scholar
  14. Chaohua C, Gonggu Z, Lining Z, Chunsheng G, Qing T, Jianhua C, Jianguang S (2016) A rapid shoot regeneration protocol from the cotyledons of hemp (Cannabis sativa L.) Ind Crop Prod 83:61–65CrossRefGoogle Scholar
  15. Cheruvathur MK, Thomas TD (2014) High frequency multiple shoot induction from nodal segments and rhinacanthin production in the medicinal shrub Rhinacanthus nasutus (L.) Kurz. Plant Growth Regul 74:47–54CrossRefGoogle Scholar
  16. Cheruvathur MK, Kumar GK, Thomas TD (2013) Somatic embryogenesis and synthetic seed production in Rhinacanthus nasutus (L.) Kurz. Plant Cell Tissue Organ Cult 113:63–71CrossRefGoogle Scholar
  17. Chik WI, Zhu L, Fan LL, Yi T, Zhu GY, Gou XJ, Yu ZL (2015) Saussurea involucrata: a review of the botany, phytochemistry and ethnopharmacology of a rare traditional herbal medicine. J Ethnopharmacol 172:44–60CrossRefPubMedGoogle Scholar
  18. Colling J, Groenewald JH, Makunga NP (2010) Genetic alterations for increased coumarin production lead to metabolic changes in the medicinally important Pelargonium sidoides DC (Geraniaceae). Metabol Eng 12:561–572CrossRefGoogle Scholar
  19. Dar MA, Masoodi MH, Wali AF, Mir MA, Shapoo NS (2014) Antioxidant potential of methanol root extract of Mentha arvensis L. from Kashmir region. J Appl Pharm Sci 4:50–57Google Scholar
  20. Do Thi N, Hwang ES (2014) Bioactive compound contents and antioxidant activity in Aronia (Aronia melanocarpa) leaves collected at different growth stages. Prev Nutr Food Sci 19:204–212CrossRefPubMedPubMedCentralGoogle Scholar
  21. Doss A, Parivuguna V, Vijayasanthi M, Surendran S (2011) Antibacterial evaluation and phytochemical analysis of Medicago sativa L. against some microbial pathogens. Indian J Sci Technol 4:550–552Google Scholar
  22. Dungerdorj D, Tsetsegmaa S, Purevsuren S, Bayasgalan B (2006) On the phytochemical investigation of the genus Gentiana used for the treatment of liver diseases in Mongolian traditional medicine. Postępy Fitoterapii 2:71–74Google Scholar
  23. Fadlalla AM, Ratnam W, Heng LY (2007) Genetic effects of arsenic and heavy metals pollutants on Curculigo latifolia (Lumbah). J Biol Sci 7:1155–1162CrossRefGoogle Scholar
  24. Faisal M, Alatar AA, Hegazy AK, Alharbi SA, El-Sheikh M, Okla MK (2014) Thidiazuron induced in vitro multiplication of Mentha arvensis and evaluation of genetic stability by flow cytometry and molecular markers. Ind Crop Prod 62:100–106CrossRefGoogle Scholar
  25. Fatima N, Ahmad N, Ahmad I, Anis M (2015) Interactive effects of growth regulators, carbon sources, ph on plant regeneration and assessment of genetic fidelity using single primer amplification reaction (SPARS) techniques in Withania somnifera L. Appl Biochem Biotechnol 177:118–136CrossRefPubMedGoogle Scholar
  26. Feng BH, Wu B, Zhang CR, Huang X, Chen YF, Huang XL (2012) Cloning and expression of 1-aminocyclopropane-1-carboxylate oxidase cDNA induced by thidiazuron during somatic embryogenesis of alfalfa (Medicago sativa). J Plant Physiol 169:176–182CrossRefPubMedGoogle Scholar
  27. Fernandez JA, Pandalai SG (2004) Biology, biotechnology and biomedicine of saffron. Rec Res Dev Plant Sci 2:127–159Google Scholar
  28. Gairi A, Rashid A (2004) Direct differentiation of somatic embryos on different regions of intact seedlings of Azadirachta in response to thidiazuron. J Plant Physiol 161:1073–1077CrossRefPubMedGoogle Scholar
  29. Gambhir G, Srivastava DK (2015) Thidiazuron induces high frequency shoot regeneration in leaf and petiole explants of cabbage (Brassica oleracea L. Var. Capitata). J Biotechnol Biomater 5:1–6Google Scholar
  30. Gondval M, Chaturvedi P, Gaur AK (2016) Thidiazuron-induced high frequency establishment of callus cultures and plantlet regeneration in Aconitum balfourii Stapf.: an endangered medicinal herb of North-West Himalayas. Indian J Biotechnol 15:251–255Google Scholar
  31. Guo B, Gao M, Liu CZ (2007) In vitro propagation of an endangered medicinal plant Saussurea involucrata Kir. Plant Cell Rep 26:261–265CrossRefPubMedGoogle Scholar
  32. Guo B, Abbasi BH, Zeb A, Xu LL, Wei YH (2011) Thidiazuron: a multi-dimensional plant growth regulator. Afr J Biotechnol 10:8984–9000CrossRefGoogle Scholar
  33. Guo B, Stiles AR, Liu CZ (2012) Thidiazuron enhances shoot organogenesis from leaf explants of Saussurea involucrata Kar et. Kir. In Vitro Cell Dev Biol Plant 48:609–612CrossRefGoogle Scholar
  34. Guo B, He W, Zhao Y, Wu Y, Fu Y, Guo J, Wei Y (2016) Changes in endogenous hormones and H2O2 burst during shoot organogenesis in TDZ-treated Saussurea involucrata explants. Plant Cell Tissue Organ Cult 128:1–8CrossRefGoogle Scholar
  35. Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult 33:105–119CrossRefGoogle Scholar
  36. Ismail MF, Abdullah NAP, Saleh G, Ismail M (2010) Anthesis and flower visitors in Curculigo latifolia Dryand (Hypoxidaceae). J Biol Life Sci 1:13–15Google Scholar
  37. Jain MN, Dhal CC, Shroff RH, Bhutada RH, Somani RS, Kasture VS, Kasture SB (2003a) Clitoria ternatea and the CNS. Pharmacol Biochem Behav 75:529–536CrossRefPubMedGoogle Scholar
  38. Jain SP, Singh J, Singh SC (2003b) Rare and endangered medicinal and aromatic plants of Madhya Pradesh. J Econ Tax Bot 27:925–932Google Scholar
  39. Jia JM, Wu CF, Liu W, Yu H, Hao Y, Zheng JH, Ji YR (2005) Anti-inflammatory and analgesic activities of the tissue culture of Saussurea involucrata. Biol Pharm Bull 28:1612–1614CrossRefPubMedGoogle Scholar
  40. Jiang BO, Yang YG, Guo YM, Guo ZC, Chen YZ (2005) Thidiazuron-induced in vitro shoot organogenesis of the medicinal plant Arnebia euchroma (Royle) Johnst. In Vitro Cell Dev Biol-Plant 41:677–681CrossRefGoogle Scholar
  41. Kant R (2005) Sweet proteins-potential replacement for artificial low calorie sweeteners. Nutrition J4:5CrossRefGoogle Scholar
  42. Kletter C, Glasl S, Thalhammer T, Narantuya S (2008) Traditional Mongolian medicine-a potential for drug discovery. Sci Pharm 76:49–63CrossRefGoogle Scholar
  43. Kodja H, Noirot M, Khoyratty SS, Limbada H, Verpoorte R, Palama TL (2015) Biochemical characterization of embryogenic calli of Vanilla planifolia in response to two years of thidiazuron treatment. Plant Physiol Biochem 96:337–344CrossRefPubMedGoogle Scholar
  44. Koizumi A, Nakajima KI, Asakura T, Morita Y, Ito K, Shmizu-Ibuka A, Abe K (2007) Taste-modifying sweet protein, neoculin, is received at human T1R3 amino terminal domain. Biochem Biophys Res Commun 358:585–589CrossRefPubMedGoogle Scholar
  45. Kokotkiewicz A, Jaremicz Z, Luczkiewicz M (2010) Aronia plants: a review of traditional use, biological activities and perspectives for modern medicine. J Med Food 13:255–269CrossRefPubMedGoogle Scholar
  46. Kumar VS, Navaratnam V (2013) Neem (Azadirachta indica): prehistory to contemporary medicinal uses to humankind. Asian Pac J Trop Biomed 3:505–514CrossRefPubMedPubMedCentralGoogle Scholar
  47. Kumar N, Reddy MP (2012) Thidiazuron (TDZ) induced plant regeneration from cotyledonary petiole explants of elite genotypes of Jatropha curcas: a candidate biodiesel plant. Ind Crop Prod 39:62–68CrossRefGoogle Scholar
  48. Kumar V, Moyo M, Gruz J, Šubrtová M, Van Staden J (2015) Phenolic acid profiles and antioxidant potential of Pelargonium sidoides callus cultures. Ind Crop Prod 77:402–408CrossRefGoogle Scholar
  49. Kunnumakkara AB, Ichikawah AP, Mohan KCJ, Hema PS, Nair MS, Aggarwal BB (2008) Coronarin D, a labdane diterpene, inhibits both constitutive and inducible nuclear factor-B κ pathway activation, leading to potentiation of apoptosis, inhibition of invasion and suppression of osteoclastogenesis. Mol Cancer Ther 7:3308–3316CrossRefGoogle Scholar
  50. Lata H, Chandra S, Khan I, ElSohly MA (2009) Thidiazuron-induced high-frequency direct shoot organogenesis of Cannabis sativa L. In Vitro Cell Dev Biol Plant 45:12–19CrossRefGoogle Scholar
  51. Lincy A, Sasikumar B (2010) Enhanced adventitious shoot regeneration from aerial stem explants of ginger using TDZ and its histological studies. Turk J Bot 34:21–29Google Scholar
  52. Mahendran G, Bai VN (2015) An efficient in vitro propagation, antioxidant and antimicrobial activities of Aphyllorchis montana Rchb f. Plant Biosyst 150:1087–1095Google Scholar
  53. Manjkhola S, Dhar U, Joshi M (2005) Organogenesis, embryogenesis and synthetic seed production in Arnebia euchroma- a critically endangered medicinal plant of the Himalaya. In Vitro Cell Dev Biol-Plant 41:244–248CrossRefGoogle Scholar
  54. McKendrick SL, Leake JR, Taylor DL, Read D (2002) Symbiotic germination and development of the mycoheterotrophic orchid Neottia nidus-Avis in nature and its requirement for locally distributed Sebacina spp. New Phytol 154:233–247CrossRefGoogle Scholar
  55. Mickienė R, Ragažinskienė O, Bakutis B (2011) Antimicrobial activity of Mentha arvensis L. and Zingiber officinale R. essential oils. Biologija 57:92–97Google Scholar
  56. Mok MC, Mok DWS, Armstrong DJ, Shudo K, Isogai Y, Okamoto T (1982) Cytokinin activity of N-phenyl-N′-1, 2, 3-thiadiazol-5-ylurea (thidiazuron). Phytochemistry 21:1509–1511CrossRefGoogle Scholar
  57. Mukherjee PK, Kumar V, Kumar NS, Heinrich M (2008) The Ayurvedic medicine Clitoria ternatea-from traditional use to scientific assessment. J Ethnopharmacol 120:291–301CrossRefPubMedGoogle Scholar
  58. Mukhtar S, Ahmad N, Khan MI, Anis M, Aref IM (2012) Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types. Physiol Mol Biol Plants 18:381–386CrossRefPubMedPubMedCentralGoogle Scholar
  59. Murch SJ, Saxena PK (1997) Modulation of mineral and free fatty acid profiles during thidiazuron mediated somatic embryogenesis in peanuts (Arachis hypogaea L.) J Plant Physiol 151:358–361CrossRefGoogle Scholar
  60. Murthy BNS, Murch SJ, Saxena PK (1998) Thidiazuron: a potent regulator of in vitro plant morphogenesis. In Vitro Cell Dev Biol Plant 34:267–275CrossRefGoogle Scholar
  61. Myagmar BE, Aniya Y (2000) Free radical scavenging action of medicinal herbs from Mongolia. Phytomedicine 7:221–229CrossRefPubMedGoogle Scholar
  62. Ojewole JA (2006) Analgesic, anti-inflammatory and hypoglycemic effects of ethanol extract of Zingiber officinale (Roscoe) rhizomes (Zingiberaceae) in mice and rats. Phytother Res 20:764–772CrossRefPubMedGoogle Scholar
  63. Oszmiański J, Wojdylo A (2005) Aronia melanocarpa phenolics and their antioxidant activity. Eur Food Res Technol 221:809–813CrossRefGoogle Scholar
  64. Pan X, Niu G, Liu H (2001) Microwave-assisted extraction of tanshinones from Salvia miltiorrhiza Bunge with analysis by high-performance liquid chromatography. J Chromatogr A 922:371–375CrossRefPubMedGoogle Scholar
  65. Panday NK, Tewari KC, Tewari RN, Joshi GC, Pande VN, Pandey G (1993) Medicinal plants of Kumaon Himalaya: strategies for conservation. In: Dhar U (ed) Himalayan biodiversity conservation strategies, no. 3. Himavikas, Nanital, pp 293–302Google Scholar
  66. Patel PS, Joshee N, Rimando AM, Parajuli P (2013) Anti-cancer scopes and associated mechanisms of Scutellaria extract and flavonoid wogonin. Curr Cancer Ther Rev 9:34–42Google Scholar
  67. Patial V, Sharma M, Bhattacharya A (2016) Potential of thidiazuron in improved micropropagation of Picrorhiza kurroa – an endangered medicinal herb of alpine Himalaya. Plant Biosyst 151:729–736CrossRefGoogle Scholar
  68. Pentela B, Thalla S, Tharangini K, Geethanjali J, Govinda RT, Venkata Lakshmi D (2012) Hypoglycemic activity of ethanolic extract of Aphyllorchis montana on Alloxan induce diabetes in rats. Int J Chem Pharm Sci 3:27–29Google Scholar
  69. Prajapati NS, Purohit SS, Sharma AK, Kumar T (2003) A handbook of medicinal plants. A complete source book. Agrobios, Jodhpur, pp 110–115Google Scholar
  70. Prasad DR, Izam A, Khan MMR (2012) Jatropha curcas: plant of medical benefits. J Med Plant Res 6:2691–2699Google Scholar
  71. Qureshi RA, Gilani SA, Ashraf M (2007) Ethnobotanical studies with special reference to plant phenology at Sudhan Gali and Ganga Chotti Hills (District Bagh, AK). Electron J Environ Agric Food Chem 6:2207–2215Google Scholar
  72. Sehgal R, Chauhan A, Gilhotra UK, Gilhotra A (2013) In vitro and in vivo evaluation of anti-asthmatic activity of Picrorhiza kurroa plant. Int J Pharm Sci Res 4:3440–3443Google Scholar
  73. Sharifi G, Ebrahimzadeh H, Ghareyazie B, Karimi M (2010) Globular embryo-like structures and highly efficient thidiazuron-induced multiple shoot formation in saffron (Crocus sativus L.) In Vitro Cell Dev Biol Plant 46:274–280CrossRefGoogle Scholar
  74. Sharma E, Gaur AK (2012) Aconitum balfourii Stapf: a rare medicinal herb from Himalayan alpine. J Med Plant Res 6:3810–3817Google Scholar
  75. Siddique I, Anis M (2006) Thidiazuron induced high frequency shoot bud formation and plant regeneration from cotyledonary node explants of Capsicum annuum L. Indian J Biotechnol 5:303–308Google Scholar
  76. Singh NR, Singh MS (2009) Wild medicinal plants of Manipur included in the red list. Asian Agrihist 13:221–225Google Scholar
  77. Sinha S, Bhat J, Joshi M, Sinkar V, Ghaskadbi S (2011) Hepatoprotective activity of Picrorhiza kurroa Royle Ex. Benth extract against alcohol cytotoxicity in mouse liver slice culture. Int J Green Pharm 5:244–253CrossRefGoogle Scholar
  78. Sinu PA, Sinu N, Chandrashekara K (2012) Ecology and population structure of a terrestrial mycoheterotrophic orchid, Aphyllorchis montana Rchb. f. (Orchidaceae) in Soppinabetta forests of the Western Ghats, India. J Threat Taxa 4:2915–2919CrossRefGoogle Scholar
  79. Sivanesan I, Saini RK, Kim DH (2016) Bioactive compounds in hyperhydric and normal micropropagated shoots of Aronia melanocarpa (Michx.) Elliott. Ind Crop Prod 83:31–38CrossRefGoogle Scholar
  80. Sreenu T, Venkata Ramana K, Jyothibasu T, Delhiraj N (2013) Hypoglycemic activity of ethanolic extract of Aphyllorchis montana induced by streptozocin in rats. Int J Pharm Tech Res 5:320–323Google Scholar
  81. Thomas TD (2003) Thidiazuron induced multiple shoot induction and plant regeneration from cotyledonary explants of mulberry. Biol Plant 46:529–533CrossRefGoogle Scholar
  82. Thomas TD, Philip B (2005) Thidiazuron-induced high-frequency shoot organogenesis from leaf-derived callus of a medicinal climber, Tylophora indica (Burm. F.) Merrill. In Vitro Cell Dev Biol Plant 41:124–128CrossRefGoogle Scholar
  83. Thomas TD, Puthur JT (2004) Thidiazuron induced high frequency shoot organogenesis in callus from Kigelia pinnata L. Bot Bull Acad Sinica 45:307–313Google Scholar
  84. Tomiczak K, Mikuła A, Sliwinska E, Rybczyński JJ (2015) Autotetraploid plant regeneration by indirect somatic embryogenesis from leaf mesophyll protoplasts of diploid Gentiana decumbens Lf. In Vitro Cell Dev Biol Plant 51:350–359CrossRefPubMedPubMedCentralGoogle Scholar
  85. Tsai KL, Chen EG, Chen JT (2016) Thidiazuron-induced efficient propagation of Salvia miltiorrhiza through in vitro organogenesis and medicinal constituents of regenerated plants. Acta Physiol Plant 38:29CrossRefGoogle Scholar
  86. Turaskar A, More S, Sheikh R, Gadhpayle J, Bongade SI (2013) Inhibitory potential of Picrorhiza kurroa Royle Ex. Benth extracts on phenylhydrazine induced reticulocytosis in rats. Asian J Pharm Clin Res 6:215–216Google Scholar
  87. Umadevi M (2012) Traditional and medicinal uses of Withania somnifera. The Pharma Innov 1:102–110Google Scholar
  88. Upadhyay B, Roy S, Kumar A (2007) Traditional uses of medicinal plants among the rural communities of Churu district in the Thar Desert, India. J Ethnopharmacol 113:387–399CrossRefPubMedGoogle Scholar
  89. Vaidya BN, Jackson CL, Perry ZD, Dhekney SA, Joshee N (2016) Agrobacterium-mediated transformation of thin cell layer explants of Scutellaria ocmulgee small: a rare plant with anti-tumor properties. Plant Cell Tissue Organ Cult 127:57–69CrossRefGoogle Scholar
  90. Van Waes JM, Debergh PC (1986) In vitro germination of some western European orchids. Physiol Plant 67:253–261CrossRefGoogle Scholar
  91. Verma M, Bansal YK (2014) Effect of a potent cytokinin thidiazuron (TDZ) on in vitro regeneration of Hedychium coronarium J Koenig-A valuable medicinal plant. Int J Rec Biotech 2:38–44Google Scholar
  92. Wang J, Xiong X, Feng B (2013) Cardiovascular effects of salvianolic acid B. Evid Based Complement Alternat Med 2013:1–16Google Scholar
  93. Warcup JH (1973) Symbiotic germination of some Australian terrestrial orchids. New Phytol 72:387–392CrossRefGoogle Scholar
  94. Yamashita H, Theerasilp S, Aiuchi T, Nakaya K, Nakamura Y, Kurihara Y (1990) Purification and complete amino acid sequence of a new type of sweet protein with taste-modifying activity, curculin. J Biolchem 265:15770–15775Google Scholar

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© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Department of Plant Science, Riverside Transit CampusCentral University of KeralaKasaragodIndia

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