Thidiazuron (TDZ): A Callus Minimizer for In Vitro Plant Production

  • Buhara Yücesan


Thidiazuron (TDZ) seems to be quite suitable growth regulator for rapid and effective plant production in vitro. In many woody and herbaceous plants, lower TDZ concentrations induce less callus production around the explant. However, there is still insufficient number of studies how actually TDZ is effective on plant production in vitro and which biochemical and molecular genetic mechanism are involved in developmental physiology. Recent studies have shown that TDZ affects endogenous cytokinin and auxin production, and therefore morphogenetic identification of cells and tissues is downregulated by various genes acting on auxin regulation and transport as well as cytokinin response. These hormones that take a role in several metabolic pathways wherein more researches are required to identify the direct regulator and stress signaling factors during plant regeneration are induced by TDZ. A closer look on these subjects may bring about a better understanding on why urea derivative TDZ is more effective than adenine types of cytokinin on most species and how low concentration of TDZ has been found to be useful for micropropagation, especially in wood plants. Lastly, why does long exposure of TDZ have not been recommended and what are the possible reasons of hyperhydricity, abnormal shoot growth, and difficulty in rooting? The answers will be quite useful for management of culture conditions for the large-scale production and conservation of economical plants.


TDZ Thidiazuron Callus Growth Regulators Morphogeny 


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© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Department of Seed Science and Technology, Faculty of Natural and Agricultural SciencesAbant İzzet Baysal UniversityBoluTurkey

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