Thidiazuron-Induced Protocorm-Like Bodies in Orchid: Progress and Prospects

  • Suprabuddha Kundu
  • Saikat Gantait


Thidiazuron (N-phenyl-N′-l,2,3-thidiazol-5-ylurea, TDZ) is a substituted phenylurea chemical compound that was initially produced for mechanical harvesting of cotton bolls. Since recent past, it has become well known as a successful plant growth regulator (PGR) in plant cell, tissue and organ culture. The unique property of TDZ mimicking both auxin and cytokinin stimulates organogenic and callogenic growth of explants. In this chapter, numerous of such potentials are discussed, considering the recently published reports on characterization of TDZ-induced orchid protocorm-like bodies (PLBs) and their subsequent conversion. The summarized results clearly exhibit that TDZ significantly influences diverse culture responses starting from induction of PLB from a wide array of explants, rate of shoot formation, shoot number per explant and the time duration needed for PLB formation compared to other PGRs. A very low concentration (<1 μM ) of TDZ is much effective in comparison to many other PGRs; though TDZ may hold back shoot elongation after culturing for long duration in the same medium. The unwanted side effect of TDZ is that the fasciated shoots are formed occasionally in some orchid species after prolonged culture. Besides the minor disapproval, the high proliferative activity and positive response to organogenesis, TDZ has evolved to be the most active cytokinin-like PGR for in vitro regeneration of orchid species.


Conversion Orchid PLB TDZ 



Authors acknowledge the e-library assistance from Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, West Bengal, India. We further are thankful to the anonymous reviewers and the editor of this chapter for their critical comments and suggestions.

In compliance with ethical standards

Conflict of Interest:

Declared none


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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Department of Agricultural Biotechnology, Faculty of AgricultureBidhan Chandra Krishi ViswavidyalayaNadiaIndia
  2. 2.All India Coordinated Research Project on GroundnutDirectorate of Research Bidhan Chandra Krishi ViswavidyalayaNadiaIndia
  3. 3.Department of Genetics and Plant Breeding, Faculty of AgricultureBidhan Chandra Krishi ViswavidyalayaNadiaIndia

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