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Colorimetric Sandwich Assays for Nucleic Acid Detection

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Biosensors Based on Sandwich Assays

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Abstract

Colorimetric sandwich assay has attracted wide interests in detection of nucleic acid due to the advantages of convenience and visibility. In this chapter, we summarized the development of colorimetric sandwich assay employed nanoparticles-, traditional enzyme-, and DNAzyme-based sensor, aiming at providing a general guide for designing colorimetric sandwich assay for the detection of nucleic acid. Furthermore, we discussed the challenges in the development of colorimetric sandwich assay regarding sensitivity and stability, thus offering further opportunities to develop more of robust colorimetric sandwich assay for the detection of nucleic acid.

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Authors and Affiliations

  1. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China

    Xiaoxia Hu & Quan Yuan

Authors
  1. Xiaoxia Hu
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  2. Quan Yuan
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Corresponding author

Correspondence to Quan Yuan .

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Editors and Affiliations

  1. Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, Hubei, China

    Fan Xia

  2. Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, Hubei, China

    Xiaojin Zhang

  3. Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, Hubei, China

    Xiaoding Lou

  4. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, China

    Quan Yuan

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Hu, X., Yuan, Q. (2018). Colorimetric Sandwich Assays for Nucleic Acid Detection. In: Xia, F., Zhang, X., Lou, X., Yuan, Q. (eds) Biosensors Based on Sandwich Assays. Springer, Singapore. https://doi.org/10.1007/978-981-10-7835-4_6

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