Colorimetric Sandwich Assays for Nucleic Acid Detection

  • Xiaoxia Hu
  • Quan YuanEmail author


Colorimetric sandwich assay has attracted wide interests in detection of nucleic acid due to the advantages of convenience and visibility. In this chapter, we summarized the development of colorimetric sandwich assay employed nanoparticles-, traditional enzyme-, and DNAzyme-based sensor, aiming at providing a general guide for designing colorimetric sandwich assay for the detection of nucleic acid. Furthermore, we discussed the challenges in the development of colorimetric sandwich assay regarding sensitivity and stability, thus offering further opportunities to develop more of robust colorimetric sandwich assay for the detection of nucleic acid.


Colorimetric sandwich assay Nucleic acid Detection Nanoparticles Traditional enzyme DNAzyme Sensitivity Stability 


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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.College of Chemistry and Molecular SciencesWuhan UniversityWuhanPeople’s Republic of China

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