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Fluorescence Sandwich Assays for Protein Detection

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Biosensors Based on Sandwich Assays

Abstract

The detection of protein with high sensitivity and selectivity is of great important for protein fundamental functions study and clinic diagnostics. Sandwich assays have been developed for multivalent proteins detection and have been prevailing for decades in the field of clinical diagnostics and bio-detection. The sandwich assays usually give a high sensitivity and selectivity because of the usage of a couple of match recognition probe and signal probe. This chapter summarizes recent advances in the sandwich assays for protein detections with fluorescence as signal outputs. Different recognition or signal elements such as antibodies and aptamers and fluorescence signal reporters (organic dyes, nanomaterials, and conjugated polymers) are discussed in details in this chapter.

The original version of this chapter was revised: Foreword has been included and authors’ affiliations have been updated. The erratum to this chapter is available at https://doi.org/10.1007/978-981-10-7835-4_13

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Authors and Affiliations

  1. Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, 430074, People’s Republic of China

    Fujian Huang & Fan Xia

  2. Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, 430074, People’s Republic of China

    Fan Xia

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  1. Fujian Huang
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  2. Fan Xia
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Correspondence to Fujian Huang .

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Editors and Affiliations

  1. Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, Hubei, China

    Fan Xia

  2. Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, Hubei, China

    Xiaojin Zhang

  3. Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, Hubei, China

    Xiaoding Lou

  4. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, China

    Quan Yuan

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Huang, F., Xia, F. (2018). Fluorescence Sandwich Assays for Protein Detection. In: Xia, F., Zhang, X., Lou, X., Yuan, Q. (eds) Biosensors Based on Sandwich Assays. Springer, Singapore. https://doi.org/10.1007/978-981-10-7835-4_3

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