Skip to main content
SpringerLink
Log in

Biosensors Based on Supersandwich Assays

  • Chapter
  • First Online:
Biosensors Based on Sandwich Assays

  • 1213 Accesses

Abstract

Only one signal probe is usually bound to the target in traditional sandwich assays, which limits the detection sensitivity. To overcome this limitation, supersandwich assays amplifying the signal through integration of multiple signal probes together have been developed in recent years. In this chapter, we highlight biosensors based on supersandwich assays for the detection of proteins, nucleic acids, small molecules, ions, and cells by a series of efforts reported in the past decade. The detection technologies employed in design of biosensors based on supersandwich assays contain electrochemical assay, electrochemiluminescence assay, fluorescence assay, and surface plasmon resonance assay.

The original version of this chapter was revised: Foreword has been included and authors’ affiliations have been updated. The erratum to this chapter is available at https://doi.org/10.1007/978-981-10-7835-4_13

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 16.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 54.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Shen JW, Li YB, Gu HS, Xia F, Zuo XL (2014) Recent development of sandwich assay based on the nanobiotechnologies for proteins, nucleic acids, small molecules, and ions. Chem Rev 114:7631–7677

    Article  CAS  Google Scholar 

  2. Xia F, White RJ, Zuo XL, Patterson A, Xiao Y, Kang D, Gong X, Plaxco KW, Heeger AJ (2010) An electrochemical supersandwich assay for sensitive and selective DNA detection in complex matrices. J Am Chem Soc 132:14346–14348

    Article  CAS  Google Scholar 

  3. Liu NN, Huang FJ, Lou XD, Xia F (2017) DNA hybridization chain reaction and DNA supersandwich self-assembly for ultrasensitive detection. Sci China-Chem 60:311–318

    Article  CAS  Google Scholar 

  4. Wang GF, Huang H, Wang BJ, Zhang XJ, Wang L (2012) A supersandwich multienzyme-DNA label based electrochemical immunosensor. Chem Commun 48:720–722

    Article  CAS  Google Scholar 

  5. Wang GF, He XP, Wang L, Zhang XJ (2013) A folate receptor electrochemical sensor based on terminal protection and supersandwich DNAzyme amplification. Biosens Bioelectron 42:337–341

    Article  CAS  Google Scholar 

  6. Wang GF, He XP, Zhu YH, Chen L, Wang L, Zhang XJ (2013) G-quadruplex-linked supersandwich DNA structure for electrochemical amplified detection of thrombin. Electroanalysis 25:1960–1966

    Article  CAS  Google Scholar 

  7. Wang Q, Liu W, Xing YQ, Yang XH, Wang KM, Jiang R, Wang P, Zhao Q (2014) Screening of DNA aptamers against myoglobin using a positive and negative selection units integrated microfluidic chip and its biosensing application. Anal Chem 86:6572–6579

    Article  CAS  Google Scholar 

  8. Liu HP, Chen Y, He Y, Ribbe AE, Mao CD (2006) Approaching the limit: can one DNA oligonucleotide assemble into large nanostructures? Angew Chem Int Ed 45:1942–1945

    Article  CAS  Google Scholar 

  9. Song YJ, Qu KG, Zhao C, Ren JS, Qu XG (2010) Graphene oxide: Intrinsic peroxidase catalytic activity and its application to glucose detection. Adv Mater 22:2206–2210

    Article  CAS  Google Scholar 

  10. Chen L, Sha L, Qiu YW, Wang GF, Jiang H, Zhang XJ (2015) An amplified electrochemical aptasensor based on hybridization chain reactions and catalysis of silver nanoclusters. Nanoscale 7:3300–3308

    Article  CAS  Google Scholar 

  11. Zhou FY, Yao Y, Luo JJ, Zhang X, Zhang Y, Yin DY, Gao FL, Wang P (2017) Proximity hybridization-regulated catalytic DNA hairpin assembly for electrochemical immunoassay based on in situ DNA template-synthesized Pd nanoparticles. Anal Chim Acta 969:8–17

    Article  CAS  Google Scholar 

  12. Smith ZD, Meissner A (2013) DNA methylation: roles in mammalian development. Nat Rev Genet 14:204–220

    Article  CAS  Google Scholar 

  13. Klutstein M, Nejman D, Greenfield R, Cedar H (2016) DNA methylation in cancer and aging. Cancer Res 76:3446–3450

    Article  CAS  Google Scholar 

  14. Edwards JR, Yarychkivska O, Boulard M, Bestor TH (2017) DNA methylation and DNA methyltransferases. Epigenetics Chromatin 10:23

    Article  Google Scholar 

  15. Duan XR, Liu LB, Feng FD, Wang S (2010) Cationic conjugated polymers for optical detection of DNA methylation, lesions, and single nucleotide polymorphisms. Acc Chem Res 43:260–270

    Article  CAS  Google Scholar 

  16. Flusberg BA, Webster DR, Lee JH, Travers KJ, Olivares EC, Clark TA, Korlach J, Turner SW (2010) Direct detection of DNA methylation during single-molecule, real-time sequencing. Nat Methods 7:461–465

    Article  CAS  Google Scholar 

  17. Li Y, Luo XE, Yan Z, Zheng JB, Qi HL (2013) A label-free supersandwich electrogenerated chemiluminescence method for the detection of DNA methylation and assay of the methyltransferase activity. Chem Commun 49:3869–3871

    Article  CAS  Google Scholar 

  18. Sun HP, Ma SX, Li Y, Qi HL (2017) Electrogenerated chemiluminescence biosensing method for the detection of DNA demethylase activity: combining MoS2 nanocomposite with DNA supersandwich. Sens Actuator B-Chem 244:885–890

    Article  CAS  Google Scholar 

  19. Jiang BY, Yang ML, Yang CY, Xiang Y, Yuan R (2017) Methylation-induced inactivation of restriction enzyme for amplified and signal-on electrochemiluminescence detection of methyltransferase activity. Sens Actuator B-Chem 247:573–579

    Article  CAS  Google Scholar 

  20. Lei R, Wang XY, Zhu SF, Li N (2011) A novel electrochemiluminescence glucose biosensor based on alcohol-free mesoporous molecular sieve silica modified electrode. Sens Actuator B-Chem 158:124–129

    Article  CAS  Google Scholar 

  21. Gui GF, Zhuo Y, Chai YQ, Liao N, Zhao M, Han J, Zhu Q, Yuan R, Xiang Y (2013) Supersandwich-type electrochemiluminescenct aptasensor based on Ru(phen) 2+3 functionalized hollow gold nanoparticles as signal-amplifying tags. Biosens Bioelectron 47:524–529

    Article  CAS  Google Scholar 

  22. He Y, Chai YQ, Yuan R, Wang HJ, Bai LJ, Cao YL, Yuan YL (2013) An ultrasensitive electrochemiluminescence immunoassay based on supersandwich DNA structure amplification with histidine as a co-reactant. Biosens Bioelectron 50:294–299

    Article  CAS  Google Scholar 

  23. He Y, Chai YQ, Yuan R, Wang HJ, Bai LJ, Liao N (2014) A supersandwich electrochemiluminescence immunosensor based on mimic-intramolecular interaction for sensitive detection of proteins. Analyst 139:5209–5214

    Article  CAS  Google Scholar 

  24. Zhou LY, Zhang XY, Wang GL, Jiao XX, Luo HQ, Li NB (2012) A simple and label-free electrochemical biosensor for DNA detection based on the super-sandwich assay. Analyst 137:5071–5075

    Article  CAS  Google Scholar 

  25. Chen Y, Wang Q, Xu J, Xiang Y, Yuan R, Chai YQ (2013) A new hybrid signal amplification strategy for ultrasensitive electrochemical detection of DNA based on enzyme-assisted target recycling and DNA supersandwich assemblies. Chem Commun 49:2052–2054

    Article  CAS  Google Scholar 

  26. Wang J, Shi AQ, Fang X, Han XW, Zhang YZ (2014) Ultrasensitive electrochemical supersandwich DNA biosensor using a glassy carbon electrode modified with gold particle-decorated sheets of graphene oxide. Microchim Acta 181:935–940

    Article  CAS  Google Scholar 

  27. Wang J, Shi AQ, Fang X, Han XW, Zhang YZ (2015) An ultrasensitive supersandwich electrochemical DNA biosensor based on gold nanoparticles decorated reduced graphene oxide. Anal Biochem 469:71–75

    Article  CAS  Google Scholar 

  28. Wei BM, Liu NN, Zhang JT, Ou XW, Duan RX, Yang ZK, Lou XD, Xia F (2015) Regulation of DNA self-assembly and DNA hybridization by chiral molecules with corresponding biosensor applications. Anal Chem 87:2058–2062

    Article  CAS  Google Scholar 

  29. Ren W, Zhou LY, Zhang Y, Li NB, Luo HQ (2016) A reusable and label-free supersandwich biosensor for sensitive DNA detection by immobilizing target-triggered DNA concatamers on ternary self-assembled monolayer. Sens Actuator B-Chem 223:24–29

    Article  CAS  Google Scholar 

  30. Wei BM, Zhang TC, Ou XW, Li XC, Lou XD, Xia F (2016) Stereochemistry-guided DNA probe for single nucleotide polymorphisms analysis. ACS Appl Mater Interfaces 8:15911–15916

    Article  CAS  Google Scholar 

  31. Zhang H, Wang Q, Yang XH, Wang KM, Li Q, Li ZP, Gao L, Nie WY, Zheng Y (2017) An isothermal electrochemical biosensor for the sensitive detection of microRNA based on a catalytic hairpin assembly and supersandwich amplification. Analyst 142:389–396

    Article  CAS  Google Scholar 

  32. Liu NN, Jiang YN, Zhou YH, Xia F, Guo W, Jiang L (2013) Two-way nanopore sensing of sequence-specific oligonucleotides and small-molecule targets in complex matrices using integrated DNA supersandwich structures. Angew Chem Int Ed 52:2007–2011

    Article  CAS  Google Scholar 

  33. Ruan SP, Li ZJ, Qi HL, Gao Q, Zhang CX (2014) Label-free supersandwich electrogenerated chemiluminescence biosensor for the determination of the HIV gene. Microchim Acta 181:1293–1300

    Article  CAS  Google Scholar 

  34. Yu JH, Choi S, Dickson RM (2009) Shuttle-based fluorogenic silver-cluster biolabels. Angew Chem Int Ed 48:318–320

    Article  CAS  Google Scholar 

  35. Wang GF, Zhu YH, Chen L, Wang L, Zhang XJ (2014) Target-induced quenching for highly sensitive detection of nucleic acids based on label-free luminescent supersandwich DNA/silver nanoclusters. Analyst 139:165–169

    Article  CAS  Google Scholar 

  36. Huang J, Wang H, Yang XH, Yang YJ, Quan K, Ying L, Xie NL, Ou M, Wang KM (2016) A supersandwich fluorescence in situ hybridization strategy for highly sensitive and selective mRNA imaging in tumor cells. Chem Commun 52:370–373

    Article  CAS  Google Scholar 

  37. Homola J (2008) Surface plasmon resonance sensors for detection of chemical and biological species. Chem Rev 108:462–493

    Article  CAS  Google Scholar 

  38. Ding XJ, Yan YR, Li SQ, Zhang Y, Cheng W, Cheng Q, Ding SJ (2015) Surface plasmon resonance biosensor for highly sensitive detection of microRNA based on DNA super-sandwich assemblies and streptavidin signal amplification. Anal Chim Acta 874:59–65

    Article  CAS  Google Scholar 

  39. Wang Q, Liu RJ, Yang XH, Wang KM, Zhu JQ, He LL, Li Q (2016) Surface plasmon resonance biosensor for enzyme-free amplified microRNA detection based on gold nanoparticles and DNA supersandwich. Sens Actuator B-Chem 223:613–620

    Article  CAS  Google Scholar 

  40. Liu RJ, Wang Q, Li Q, Yang XH, Wang KM, Nie WY (2017) Surface plasmon resonance biosensor for sensitive detection of microRNA and cancer cell using multiple signal amplification strategy. Biosens Bioelectron 87:433–438

    Article  CAS  Google Scholar 

  41. Patel A, Malinovska L, Saha S, Wang J, Alberti S, Krishnan Y, Hyman AA (2017) ATP as a biological hydrotrope. Science 356:753–756

    Article  CAS  Google Scholar 

  42. Ma CB, Chen HC, Han R, He HL, Zeng WM (2012) Fluorescence detection of adenosine triphosphate using smart probe. Anal Biochem 429:8–10

    Article  CAS  Google Scholar 

  43. Huo Y, Qi L, Lv XJ, Lai T, Zhang J, Zhang ZQ (2016) A sensitive aptasensor for colorimetric detection of adenosine triphosphate based on the protective effect of ATP-aptamer complexes on unmodified gold nanoparticles. Biosens Bioelectron 78:315–320

    Article  CAS  Google Scholar 

  44. Wei BM, Zhang JT, Wang HB, Xia F (2016) A new electrochemical aptasensor based on a dual-signaling strategy and supersandwich assay. Analyst 141:4313–4318

    Article  CAS  Google Scholar 

  45. Jiang YN, Liu NN, Guo W, Xia F, Jiang L (2012) Highly-efficient gating of solid-state nanochannels by DNA supersandwich structure containing ATP aptamers: a nanofluidic implication logic device. J Am Chem Soc 134:15395–15401

    Article  CAS  Google Scholar 

  46. Yang XH, Zhu JQ, Wang Q, Wang KM, Yang LJ, Zhu HZ (2012) A label-free and sensitive supersandwich electrochemical biosensor for small molecule detection based on target-induced aptamer displacement. Anal Methods 4:2221–2223

    Article  CAS  Google Scholar 

  47. Wang GF, He XP, Chen L, Zhu YH, Zhang XJ, Wang L (2013) Conformational switch for cisplatin with hemin/G-quadruplex DNAzyme supersandwich structure. Biosens Bioelectron 50:210–216

    Article  CAS  Google Scholar 

  48. Ono A, Togashi H (2004) Highly selective oligonucleotide-based sensor for mercury(II) in aqueous solutions. Angew Chem Int Ed 43:4300–4302

    Article  CAS  Google Scholar 

  49. Wang GF, He XP, Wang BJ, Zhang XJ, Wang L (2012) Electrochemical amplified detection of Hg2+ based on the supersandwich DNA structure. Analyst 137:2036–2038

    Article  CAS  Google Scholar 

  50. AshaRani PV, Mun GLK, Hande MP, Valiyaveettil S (2009) Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano 3:279–290

    Article  CAS  Google Scholar 

  51. Xu G, Wang GF, He XP, Zhu YH, Chen L, Zhang XJ (2013) An ultrasensitive electrochemical method for detection of Ag+ based on cyclic amplification of exonuclease III activity on cytosine-Ag+-cytosine. Analyst 138:6900–6906

    Article  CAS  Google Scholar 

  52. Zhang YL, Li HY, Chen M, Fang X, Pang PF, Wang HB, Wu Z, Yang WR (2017) Ultrasensitive electrochemical biosensor for silver ion based on magnetic nanoparticles labeling with hybridization chain reaction amplification strategy. Sens Actuator B-Chem 249:431–438

    Article  CAS  Google Scholar 

  53. Liu NN, Hou RZ, Gao PC, Lou XD, Xia F (2016) Sensitive Zn2+ sensor based on biofunctionalized nanopores via combination of DNAzyme and DNA supersandwich structures. Analyst 141:3626–3629

    Article  CAS  Google Scholar 

  54. Chen Y, Yang ML, Xiang Y, Yuan R, Chai YQ (2014) Binding-induced autonomous disassembly of aptamer-DNAzyme supersandwich nanostructures for sensitive electrochemiluminescence turn-on detection of ochratoxin A. Nanoscale 6:1099–1104

    Article  CAS  Google Scholar 

  55. Yuan T, Liu ZY, Hu LZ, Zhang L, Xu GB (2011) Label-free supersandwich electrochemiluminescence assay for detection of sub-nanomolar Hg2+. Chem Commun 47:11951–11953

    Article  CAS  Google Scholar 

  56. Lin CS, Chen YY, Cai ZX, Zhu Z, Jiang YQ, Yang CJ, Chen X (2015) A label-free fluorescence strategy for sensitive detection of ATP based on the ligation-triggered super-sandwich. Biosens Bioelectron 63:562–565

    Article  CAS  Google Scholar 

  57. Yuan T, Hu LZ, Liu ZY, Qi WJ, Zhu SY, Aziz ur R, Xu GB (2013) A label-free and signal-on supersandwich fluorescent platform for Hg2+ sensing. Anal Chim Acta 793:86–89

    Article  CAS  Google Scholar 

  58. Galanzha EI, Shashkov EV, Kelly T, Kim JW, Yang LL, Zharov VP (2009) In vivo magnetic enrichment and multiplex photoacoustic detection of circulating tumour cells. Nat Nanotechnol 4:855–860

    Article  CAS  Google Scholar 

  59. Liu HY, Xu SM, He ZM, Deng AP, Zhu JJ (2013) Supersandwich cytosensor for selective and ultrasensitive detection of cancer cells using aptamer-DNA concatamer-quantum dots probes. Anal Chem 85:3385–3392

    Article  CAS  Google Scholar 

  60. Lu CY, Xu JJ, Wang ZH, Chen HY (2015) A novel signal-amplified electrochemical aptasensor based on supersandwich G-quadruplex DNAzyme for highly sensitive cancer cell detection. Electrochem Commun 52:49–52

    Article  CAS  Google Scholar 

  61. Li N, Xiao TY, Zhang ZT, He RX, Wen D, Cao YP, Zhang WY, Chen Y (2015) A 3D graphene oxide microchip and a Au-enwrapped silica nanocomposite-based supersandwich cytosensor toward capture and analysis of circulating tumor cells. Nanoscale 7:16354–16360

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, 430074, People’s Republic of China

    Xiaojin Zhang & Fan Xia

  2. Hubei Key Laboratory of Bioinorganic Chemistryand Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, 430074, People’s Republic of China

    Fan Xia

Authors
  1. Xiaojin Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fan Xia
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xiaojin Zhang .

Editor information

Editors and Affiliations

  1. Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, Hubei, China

    Fan Xia

  2. Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, Hubei, China

    Xiaojin Zhang

  3. Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, Hubei, China

    Xiaoding Lou

  4. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, China

    Quan Yuan

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Nature Singapore Pte Ltd.

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Zhang, X., Xia, F. (2018). Biosensors Based on Supersandwich Assays. In: Xia, F., Zhang, X., Lou, X., Yuan, Q. (eds) Biosensors Based on Sandwich Assays. Springer, Singapore. https://doi.org/10.1007/978-981-10-7835-4_12

Download citation

Publish with us

Policies and ethics

Search

Navigation