• Xiaojin Zhang
  • Fan XiaEmail author


The sandwich assays are one of the mainstays in the fields of clinical diagnostics, molecular detection, and environmental monitoring due to their high specificity and good sensitivity for the detection of analytes. Owing to the development of chemistry and material science, the sandwich assays have been developed vigorously with thousands of published papers to date. To further improve the sensitivity, supersandwich assays emerge as the times require. In this chapter, we will introduce the sandwich assays and briefly discuss the applications of the sandwich assays in the detection of proteins, nucleic acids, small molecules, ions, and cells as well as supersandwich assays. The discussion in detail can be found in subsequent chapters.


Sandwich assays Detection of analytes Supersandwich assays High specificity Good sensitivity 


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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and ChemistryChina University of GeosciencesWuhanPeople’s Republic of China
  2. 2.Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical EngineeringHuazhong University of Science and TechnologyWuhanPeople’s Republic of China

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