Skip to main content
SpringerLink
Log in

Positively Charged Silver Nanoparticles as Labels for Paper-Based Colorimetric Detection of Heparin

  • Conference paper
  • First Online:
2nd International Conference for Innovation in Biomedical Engineering and Life Sciences (ICIBEL 2017)

Part of the book series: IFMBE Proceedings ((IFMBE,volume 67))

Abstract

A novel paper-based colorimetric sensor has been developed for ultrasensitive detection of heparin based on aggregation of positively charged silver nanoparticles. The electrostatic interaction between negatively charged heparin and positively charged silver nanoparticles (AgNPs) resulted in a shift in the surface plasmon resonance (SPR) wavelength leading to a color change from yellow to colorless. The proposed assay can selectively detect heparin with an ultra-low detection limit of 1.898 ng mL−1 in solution and 8.68 µg mL−1 on paper. In future, the assay can be further employed for detection of heparin in human blood serum samples.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Cao, R., Li, B.: A simple and sensitive method for visual detection of heparin using positively-charged gold nanoparticles as colorimetric probes. Chem. Commun. 47(10), 2865–2867 (2011)

    Article  Google Scholar 

  2. Rabenstein, D.L.: Heparin and heparan sulfate: structure and function. Nat. Prod. Rep. 19(3), 312–331 (2002)

    Article  Google Scholar 

  3. Zhan, R., Fang, Z., Liu, B.: Naked-eye detection and quantification of heparin in serum with a cationic polythiophene. Anal. Chem. 82(4), 1326–1333 (2009)

    Article  Google Scholar 

  4. Meyerhoff, M.E., Fu, B., Bakker, E., Yun, J.H., Yang, V.C.: Peer reviewed: polyion-sensitive membrane electrodes for biomedical analysis. Anal. Chem. 68(5), 168A–175A (1996)

    Article  Google Scholar 

  5. Patel, R.P., Narkowicz, C., Jacobson, G.A.: Effective reversed-phase ion pair high-performance liquid chromatography method for the separation and characterization of intact low-molecular-weight heparins. Anal. Biochem. 387(1), 113–121 (2009)

    Article  Google Scholar 

  6. Ander, B., Karlsson, A., Öhrlund, Å.: Determination of heparin on intraocular lens surfaces by ion chromatography. J. Chromatogr. A 917(1), 105–110 (2001)

    Article  Google Scholar 

  7. Murray, D.J., Brosnahan, W.J., Pennell, B., Kapalanski, D., Weiler, J.M., Olson, J.: Heparin detection by the activated coagulation time: a comparison of the sensitivity of coagulation tests and heparin assays. J. Cardiothorac. Vasc. Anesth. 11(1), 24–28 (1997)

    Article  Google Scholar 

  8. Gemene, K.L., Meyerhoff, M.E.: Reversible detection of heparin and other polyanions by pulsed chronopotentiometric polymer membrane electrode. Anal. Chem. 82(5), 1612–1615 (2010)

    Article  Google Scholar 

  9. Pu, K.Y., Liu, B.: Conjugated polyelectrolytes as light-up macromolecular probes for heparin sensing. Adv. Funct. Mater. 19(2), 277–284 (2009)

    Article  Google Scholar 

  10. Zhang, Z., Li, B., Suwan, J., Zhang, F., Wang, Z., Liu, H., Mulloy, B., Linhardt, R.J.: Analysis of pharmaceutical heparins and potential contaminants using 1H-NMR and PAGE. J. Pharm. Sci. 98(11), 4017–4026 (2009)

    Article  Google Scholar 

  11. Yan, H., Wang, H.F.: Turn-on room temperature phosphorescence assay of heparin with tunable sensitivity and detection window based on target-induced self-assembly of polyethyleneimine capped Mn-doped ZnS quantum dots. Anal. Chem. 83(22), 8589–8595 (2011)

    Article  Google Scholar 

  12. Chen, Z., Wang, Z., Chen, X., Xu, H., Liu, J.: Chitosan-capped gold nanoparticles for selective and colorimetric sensing of heparin. J. Nanopart. Res. 15(9), (2013)

    Google Scholar 

  13. Fu, X., Chen, L., Li, J., Lin, M., You, H., Wang, W.: Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide. Biosens. Bioelectron. 34(1), 227–231 (2012)

    Article  Google Scholar 

  14. Fu, X., Chen, L., Li, J.: Ultrasensitive colorimetric detection of heparin based on self-assembly of gold nanoparticles on graphene oxide. Analyst 137(16), 3653–3658 (2012)

    Article  Google Scholar 

  15. Huang, H., Yuan, Q., Yang, X.: Preparation and characterization of metal–chitosan nanocomposites. Colloids Surf. B 39(1), 31–37 (2004)

    Article  Google Scholar 

  16. Jena, B.K., Raj, C.R.: Optical sensing of biomedically important polyionic drugs using nano-sized gold particles. Biosens. Bioelectron. 23(8), 1285–1290 (2008)

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by the Ministry of Human Resource Department (MHRD), India under the Design Innovation Centre (DIC) scheme (Project number-MHRD/DESP/2016142H).

Author information

Authors and Affiliations

  1. Microsystems Fabrication Laboratory, Department of Mechanical Engineering, Indian Institute of Technology Kanpur, Kanpur, India

    Sanjay Kumar, Pulak Bhushan & Shantanu Bhattacharya

  2. Design Programme, Indian Institute of Technology Kanpur, Kanpur, India

    Shantanu Bhattacharya

Authors
  1. Sanjay Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pulak Bhushan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shantanu Bhattacharya
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shantanu Bhattacharya .

Editor information

Editors and Affiliations

  1. Department of Biomedical Engineering, Faculty of Engineering, Centre for Innovation in Medical Engineering, University of Malaya, Kuala Lumpur, Malaysia

    Fatimah Ibrahim

  2. Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur, Malaysia

    Juliana Usman

  3. Department of Biomedical Engineering, Faculty of Engineering, Centre for Innovation in Medical Engineering, University of Malaya, Kuala Lumpur, Malaysia

    Mohd Yazed Ahmad

  4. Department of Rehabilitation Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia

    Norhamizan Hamzah

  5. Department of Biomedical Engineering, Faculty of Engineering, Centre for Innovation in Medical Engineering, University of Malaya, Kuala Lumpur, Malaysia

    Swe Jyan Teh

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Science+Business Media Singapore

About this paper

Check for updates. Verify currency and authenticity via CrossMark

Cite this paper

Kumar, S., Bhushan, P., Bhattacharya, S. (2018). Positively Charged Silver Nanoparticles as Labels for Paper-Based Colorimetric Detection of Heparin. In: Ibrahim, F., Usman, J., Ahmad, M., Hamzah, N., Teh, S. (eds) 2nd International Conference for Innovation in Biomedical Engineering and Life Sciences. ICIBEL 2017. IFMBE Proceedings, vol 67. Springer, Singapore. https://doi.org/10.1007/978-981-10-7554-4_41

Download citation

  • DOI: https://doi.org/10.1007/978-981-10-7554-4_41

  • Published:

  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-10-7553-7

  • Online ISBN: 978-981-10-7554-4

  • eBook Packages: EngineeringEngineering (R0)

Publish with us

Policies and ethics

Search

Navigation