Abstract
Decellularization of sonication system efficiency is dictated by evaluation of host immune response on scaffolds. This aim of this study is to examine the inflammatory response after implantation of bioscaffolds decellularized by sonication treatment at 35 days post-surgery in rats. In this study, aortic tissues decellularized by sonication treatment in 0.1 and 2% of Sodium Dodecyl Sulfate (SDS) detergents. Samples were washed for 5 days in Phosphate Buffer Saline (PBS) following decellularization. Samples were implanted in on the lower right thoracic cavity of rats for 35 days. To examine the inflammatory response, the implanted samples were explanted and evaluated histologically by Hematoxylin Eosin (H-E) staining. From the histological analysis, bioscaffolds decellularized by sonication treatment in response to macrophages shows the minimal inflammatory response. Minimal inflammatory response elicits by bioscaffolds suggests the safety of bioscaffolds prepared by sonication treatment as biomedical implants.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Sheikh, Z., Brooks, P.J., Barzilay, O., Fine, N., Glogauer, M.: Macrophages, foreign body giant cells and their response to implantable biomaterials. Materials 8(9), 5671–5701 (2015)
Syazwani, N., Azhim, A., Morimoto, Y., Furukawa, K.S., Ushida, T.: Decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering. J. Med. Biol. Eng. 35(2), 258–269 (2015)
Smith, M.J., Smith, D.C., White, K.L., Bowlin, G.L.: Immune response testing of electrospun polymers: an important consideration in the evaluation of biomaterials. J. Eng. Fibers Fabr. 2(2), 41–47 (2007)
Crapo, P.M., Gilbert, T.W., Badylak, S.F.: An overview of tissue and whole organ decellularization processes. Biomaterials 32(12), 3233–3243 (2011)
Koch, H., Graneist, C., Emmrich, F., Till, H., Metzger, R., Aupperle, H., Schierle, K., Sack, U., & Boldt, A.: Xenogenic esophagus scaffolds fixed with several agents : Comparative in vivo study of rejection an inflammation. J. Biomed. Biotechnol. 2012, 1–11 (2012)
Acknowledgements
The authors are grateful to the Ministry of Higher Education for financial support through Fundamental Research Grant Scheme (FRGS15-204-0445), Prototype Research Grant Scheme (PRGS16-002-0033) and Transdiciplinary Research Grant Scheme (TRGS16-02-001-0001).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media Singapore
About this paper
Cite this paper
Hazwani, A., Sha’ban, M., Azhim, A. (2018). Inflammatory Response of Bioscaffolds Decellularized by Sonication Treatment. In: Ibrahim, F., Usman, J., Ahmad, M., Hamzah, N., Teh, S. (eds) 2nd International Conference for Innovation in Biomedical Engineering and Life Sciences. ICIBEL 2017. IFMBE Proceedings, vol 67. Springer, Singapore. https://doi.org/10.1007/978-981-10-7554-4_32
Download citation
DOI: https://doi.org/10.1007/978-981-10-7554-4_32
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-10-7553-7
Online ISBN: 978-981-10-7554-4
eBook Packages: EngineeringEngineering (R0)