Skip to main content
SpringerLink
Log in
Book cover

Transgenic Ascidians pp 121–129Cite as

The Enhancer Trap in Ciona

  • Chapter
  • First Online:

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1029))

Abstract

Enhancer trap is a famous application of transposons. This method is useful for the creation of marker transgenic lines that express a reporter gene in tissue- or organ-specific manner, characterization of enhancers in the genome, finding novel patterns of gene expression, and mutagenesis. In Ciona intestinalis, efficient enhancer traps with Minos and Sleeping beauty transposons have been reported. With the enhancer trap lines, the intronic enhancers regulating the expression of the Musashi gene, the compartment in the digestive tube, the presence of enhancers sensitive to the orientation of the gene that they regulate, and the functions of the Hox1 gene have been revealed. The enhancer trap lines generated with the transposon vectors are valuable resources for use as visual markers.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.00
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

References

  • Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P (2008) Molecular biology of the cell, 5th ed. Garland Science, New York

    Google Scholar 

  • Awazu S, Sasaki A, Matsuoka T, Satoh N, Sasakura Y (2004) An enhancer trap in the ascidian Ciona intestinalis identifies enhancers of its Musashi orthologous gene. Dev Biol 275:459–472

    Article  CAS  PubMed  Google Scholar 

  • Awazu S, Matsuoka T, Inaba K, Satoh N, Sasakura Y (2007) High-throughput enhancer trap by remobilization of transposon Minos in Ciona intestinalis. Genesis 45:307–317

    Article  CAS  PubMed  Google Scholar 

  • Corbo JC, Levine M, Zeller RW (1997) Characterization of a notochord-specific enhancer from the Brachyury promoter region of the ascidian, Ciona intestinalis. Development 124:589–602

    CAS  PubMed  Google Scholar 

  • Dehal P, Satou Y, Campbell RK, Chapman J, Degnan B, De Tomaso A, Davidson B, Di Gregorio A, Gelpke M, Goodstein DM, Harafuji N, Hastings KE, Ho I, Hotta K, Huang W, Kawashima T, Lemaire P, Martinez D, Meinertzhagen IA, Necula S, Nonaka M, Putnam N, Rash S, Saiga H, Satake M, Terry A, Yamada L, Wang HG, Awazu S, Azumi K, Boore J, Branno M, Chin-Bow S, DeSantis R, Doyle S, Francino P, Keys DN, Haga S, Hayashi H, Hino K, Imai KS, Inaba K, Kano S, Kobayashi K, Kobayashi M, Lee BI, Makabe KW, Manohar C, Matassi G, Medina M, Mochizuki Y, Mount S, Morishita T, Miura S, Nakayama A, Nishizaka S, Nomoto H, Ohta F, Oishi K, Rigoutsos I, Sano M, Sasaki A, Sasakura Y, Shoguchi E, Shin-I T, Spagnuolo A, Stainier D, Suzuki MM, Tassy O, Takatori N, Tokuoka M, Yagi K, Yoshizaki F, Wada S, Zhang C, Hyatt PD, Larimer F, Detter C, Doggett N, Glavina T, Hawkins T, Richardson P, Lucas S, Kohara Y, Levine M, Satoh N, Rokhsar DS (2002) The draft genome of Ciona intestinalis: insight into chordate and vertebrate origins. Science 298:2157–2167

    Article  CAS  PubMed  Google Scholar 

  • Duffy JB (2002) GAL4 system in Drosophila: a fly geneticist's Swiss army knife. Genesis 34:1–15

    Article  CAS  PubMed  Google Scholar 

  • Hozumi A, Kawai N, Yoshida R, Ogura Y, Ohta N, Satake H, Satoh N, Sasakura Y (2010) Efficient transposition of a single Minos transposon copy in the genome of the ascidian Ciona intestinalis with a transgenic line expressing transposase in eggs. Dev Dyn 239:1076–1088

    Article  CAS  PubMed  Google Scholar 

  • Hozumi A, Yoshida R, Horie T, Sakuma T, Yamamoto T, Sasakura Y (2013) Enhancer activity sensitive to the orientation of the gene it regulates in the chordate genome. Dev Biol 375:79–91

    Article  CAS  PubMed  Google Scholar 

  • Ikuta T, Yoshida N, Satoh N, Saiga H (2004) Ciona intestinalis Hox gene cluster: its dispersed structure and residual colinear expression in development. Proc Natl Acad Sci U S A 101:15118–15123

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kawai N, Ogura Y, Ikuta T, Saiga H, Hamada M, Sakuma T, Yamamoto T, Satoh N, Sasakura Y (2015) Hox10-regulated endodermal strand cell migration is essential for development of the ascidian intestine. Dev Biol 403:43–56

    Article  CAS  PubMed  Google Scholar 

  • Kawakami K (2005) Transposon tools and methods in zebrafish. Dev Dyn 234:244–254

    Article  CAS  PubMed  Google Scholar 

  • Nakamura M, Okano H, Blendy JA, Montell C (1994) Musashi, a neural RNA-binding protein required for Drosophila adult external sensory organ development. Neuron 13:67–81

    Article  CAS  PubMed  Google Scholar 

  • Ogasawara M, Di Lauro R, Satoh N (1999) Ascidian homologs of mammalian thyroid peroxidase genes are expressed in the thyroid-equivalent region of the endostyle. J Exp Zool 285:158–169

    Article  CAS  PubMed  Google Scholar 

  • Ohta N, Horie T, Satoh N, Sasakura Y (2010) Transposon-mediated enhancer detection reveals the location, morphology and development of the cupular organs, which are putative hydrodynamic sensors, in the ascidian Ciona intestinalis. Zool Sci 27:842–850

    Article  CAS  PubMed  Google Scholar 

  • O'Kane C, Gehring WJ (1987) Detection in situ of genomic regulatory elements in Drosophila. Proc Natl Acad Sci U S A 84:9123–9127

    Article  PubMed  PubMed Central  Google Scholar 

  • Sasakura Y, Awazu S, Chiba S, Satoh N (2003) Germ-line transgenesis of the Tc1/mariner superfamily transposon Minos in Ciona intestinalis. Proc Natl Acad Sci U S A 100:7726–7730

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sasakura Y, Konno A, Mizuno K, Satoh N, Inaba K (2008) Enhancer detection in the ascidian Ciona intestinalis with transposase-expressing lines of Minos. Dev Dyn 237:39–50

    Article  CAS  PubMed  Google Scholar 

  • Sasakura Y, Inaba K, Satoh N, Kondo M, Akasaka K (2009) Ciona intestinalis and Oxycomanthus japonicus, representatives of marine invertebrates. Exp Anim 58:459–469

    Article  CAS  PubMed  Google Scholar 

  • Sasakura Y, Kanda M, Ikeda T, Horie T, Kawai N, Ogura Y, Yoshida R, Hozumi A, Satoh N, Fujiwara S (2012) Retinoic acid-driven Hoxl is required in the epidermis for forming the otic/atrial placodes during ascidian metamorphosis. Development 139:2156–2160

    Google Scholar 

  • Sasakura Y, Ogura Y, Treen N, Yokomori R, Park SJ, Nakai K, Saiga H, Sakuma T, Yamamoto T, Fujiwara S, Yoshida K (2016) Transcriptional regulation of a horizontally transferred gene from bacterium to chordate. Proc Biol Sci 283:20161712

    Article  PubMed  PubMed Central  Google Scholar 

  • Willey A (1983) Studies on the Protochordata. l. On the origin of the branchial stigmata, preoral lobe, endostyle, atrial cavities, etc. in Ciona intestinalis, Linn., with remarks on Clavelina lepadiformis. Q J Micr Sci 34:317–360

    Google Scholar 

  • Yoshida R, Sasakura Y (2012) Establishment of enhancer detection lines expressing GFP in the gut of the ascidian Ciona Intestinalis. Zool Sci 29:11–20

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka, Japan

    Yasunori Sasakura

Authors
  1. Yasunori Sasakura
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yasunori Sasakura .

Editor information

Editors and Affiliations

  1. Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka, Japan

    Yasunori Sasakura

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Nature Singapore Pte Ltd.

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Sasakura, Y. (2018). The Enhancer Trap in Ciona . In: Sasakura, Y. (eds) Transgenic Ascidians . Advances in Experimental Medicine and Biology, vol 1029. Springer, Singapore. https://doi.org/10.1007/978-981-10-7545-2_11

Download citation

Publish with us

Policies and ethics

Search

Navigation