Abstract
DNA barcoding is a modern and extensively used molecular-based recognition method that aims to categorize biological specimens and to affiliate them to a given species. Newly, the progress of next-generation sequencing technology has become growingly important in the bacterial taxonomy analysis, sequence classification, and species recognition. This chapter describes the major 16S rRNA gene sequence databases and tools available for DNA barcoding studies. Here we reviewed bioinformatics, tools and methods are summarized that can support researchers who accurately prepare a database query to be capable of retrieving the most proper information required for their area of research. The aim of the present work is to draw outline of the current scenario of bacterial DNA barcoding with respect to bacterial sequence classification and species identification.
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Acknowledgments
The authors wish to thank the director of CSIR-NEERI for providing facility to do this work. RPM is thankful to SRTM University, Nanded, for Ph.D. work.
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More, R.P., Purohit, H.J. (2018). Soft Computing Methods and Tools for Bacteria DNA Barcoding Data Analysis. In: Purohit, H., Kalia, V., More, R. (eds) Soft Computing for Biological Systems. Springer, Singapore. https://doi.org/10.1007/978-981-10-7455-4_13
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