Abstract
Pathogen attack is an intricate stimulus that induces stepwise defence response, namely, pathogen recognition, signal transduction and accomplishment of resistance/defense. These steps employ an array of proteins, interacting among themselves to sense the pathogen and produce antimicrobials antagonistic to pathogen growth. In order to gain insights in molecular mechanism of plant–pathogen interaction at the biochemical and cellular level, deciphering the proteins that are involved in this cellular medley is a prerequisite. Proteomics, one of the important subjects of “OMICS” generation, has played a principal role in the identification of these proteins. Proteomics aims at identification and quantification of the proteins mediating a specific cellular process. While the current proteomic studies give valid information about these processes, they also emphasize upon the significance of post-translational modifications. The information on sequence and post-translational modifications of proteins is then used to further decipher the biological processes using bioinformatics, genomics, cell biology, biochemistry and other areas of life sciences. We present a brief overview of the proteomic studies related to host–virus, host–bacteria and host–fungus interaction. We also provide the current stage of information on the techniques applied in proteomics and also the future challenges in this area of biological science.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Abdallah C, Dumas-Gaudot E, Renaut J et al (2012) Gel-based and gel-free quantitative proteomics approaches at a glance. Int J Plant Genom 2012:Article ID 494572., 17 pages. https://doi.org/10.1155/2012/ 494572
Agarwal K, Choe LH, Lee KH (2006) Shotgun proteomics using the iTRAQ isobaric tags. Brief Funct Genom Proteomics 5(2):112–120
Agrawal GK, Jwa NS, Lebrun MH et al (2010) Plant secretome: unlocking secrets of the secreted proteins. Proteomics 10:799–827
Anderson DC, Campbell EL, Meeks JC (2006) A soluble 3D LC/MS/MS proteome of the filamentous cyanobacterium Nostoc punctiforme. J Proteome Res 5:3096–3104
Andrade AE, Silva LP, Pereira JL et al (2008) In vivo proteome analysis of Xanthomonas campestris pv. campestris in the interaction with the host plant Brassica oleracea. FEMS Microbiol Lett 281:167–174
Anguraj-Vadivel AK (2015) Gel-based proteomics in plants: time to move on from the tradition. Front Plant Sci 6:369
Asai S, Shirasu K (2015) Plant cells under siege: plant immune system versus pathogen effectors. Curr Opin Plant Biol 28:1–8
Babich R, Katam R (2016) Leaf proteome profiling and their interactions to determine disease resistance in Grape. Book of Abstract ‘Plant and Animal genome conference XXIV’ January 08–13, 2016 San Diego, CA
Bertone P, Snyder M (2005) Advances in functional protein microarray technology. FEBS J 272:5400–5411
Bohmer M, Colby T, Bohmer C et al (2007) Proteomic analysis of dimorphic transition in the phytopathogenic fungus Ustilago maydis. Proteomics 7:675–685
Boller T, Felix G (2009) A renaissance of elicitors: perception of microbe associated molecular patterns and danger signals by pattern-recognition receptors. Annu Rev Plant Biol 60:379–406
Bosch G, Skovran E, Xia Q et al (2008) Comprehensive proteomics of methylobacterium extorquens AM1 metabolism under single carbon and non methylotrophic conditions. Proteomics 8:3494–3505
Brizard JP, Carapito C, Delalande F et al (2006) Proteome analysis of plant–virus interactome: comprehensive data for virus multiplication inside their hosts. Mol Cell Proteomics 5:2279–2297
Buttner D, Bonas U (2010) Regulation and secretion of Xanthomonas virulence factors. FEMS Microbiol Rev 34:107–133
Campo S, Carrascal M, Coca M et al (2004) The defence response of germinating maize embryos against fungal infection: a proteomics approach. Proteomics 4(2):383–396
Cantin GT, Venable JD, Cociorva D et al (2006) Quantitative phosphoproteomic analysis of the tumor necrosis factor pathway. J Proteome Res 5(1):127–134
Casado-Vela J, Selles S, Martinez RB (2006) Proteomic analysis of tobacco mosaic virus-infected tomato (Lycopersicon esculentum M.) fruits and detection of viral coat protein. Proteomics 6(Suppl. 1):S196–S206
Chen F, Yuan Y, Li Q et al (2007) Proteomic analysis of rice plasma membrane reveals proteins involved in early defense response to bacterial blight. Proteomics 7:1529–1539
Chivasa S, Hamilton JM, Pringle RS et al (2006) Proteomic analysis of differentially expressed proteins in fungal elicitor-treated Arabidopsis cell cultures. J Expt Bot 57(7):1553–1562
Chung WJ, Shu HY, Lu C et al (2007) Qualitative and comparative proteomic analysis of Xanthomonas campestris pv. Campestris17. Proteomics 7:2047–2058
Coaker GL, Willard B, Kinter M et al (2004) Proteomic analysis of resistance mediated by Rcm 2.0 and Rcm 5.1, two loci controlling resistance to bacterial canker of tomato. Mol Plant-Microbe Interact 17:1019–1028
Cooper B, Clarke JD, Budworth P et al (2003) A network of rice genes associated with stress response and seed development. Proc Natl Acad Sci U S A 100:4945–4950
Corbett M, Virtue S, Bell K et al (2005) Identification of a new quorum-sensing controlled virulence factor in Erwinia carotovora subsp. Atroseptica secreted via the type II targeting pathway. Mol Plant-Microbe Interact 18:334–342
De-Blasio SL, Johnson R, Sweeney MM et al (2015) The potato leafroll virus structural proteins manipulate overlapping, yet distinct protein interaction networks during infection. Proteomics 15(12):2098–2112
Delalande F, Carapito C, Brizard JP et al (2005) Multigenic families and proteomics: extended protein characterization as a tool for paralog gene identification. Proteomics 5:450–460
Delaunois B, Jeandet P, Clément C et al (2014) Uncovering plant-pathogen crosstalk through apoplastic proteomic studies. Front Plant Sci 5(249):1–18
Demirci YE, Inan C, Gunel A et al (2016) Proteome profiling of the compatible interaction between wheat and stripe rust. Eur J Plant Pathol 145(4):941–962
Devos S, Laukens K, Deckers P et al (2006) A hormone and proteome approach to picturing the initial metabolic events during Plasmodiophora brassicae infection on Arabidopsis. Mol Plant-Microbe Interact 19:1431–1443
Diaz-Vivancos P, Rubio M, Mesonero V et al (2006) The apoplastic antioxidant system in Prunus: response to long-term plum pox virus infection. J Exp Bot 57:3813–3824
Dodds PN, Rathjen JP (2010) Plant immunity: towards an integrated view of plant– pathogen interactions. Nat Rev Genet 11(8):539–548
Doehlemann G, Hemetsberger C (2013) Apoplastic immunity and its suppression by filamentous plant pathogens. New Phytol 198:1001–1016
Duley H, Grover A (2001) Current initiatives in proteomics research: the plant perspective. Curr Sci 80(2):262–269
Ekramoddoullah AKM, Hunt RS (1993) Changes in protein profile of susceptible and resistant sugar-pine foliage infected with the Whitepine blister rust fungus Cronartium ribicola. Can J Plant Pathol 15(4):259–264
Ellis JG, Dodds PN, Lawrence GJ (2007) The role of secreted proteins in diseases of plants caused by rust, powdery mildew and smut fungi. Curr Opin Microbiol 10:326–331
Feng X, Liu BF, Li J et al (2015) Advances in coupling microfluidic chips to mass spectrometry. Mass Spectrom Rev 34(5):535–557
Fenn JB, Mann M, Meng CK et al (1989) Electrospray ionization for mass spectrometry of large biomolecules. Science 246:64–71
Fey SJ, Larsen PM (2001) 2D or not 2D two-dimensional gel electrophoresis. Curr Opin Chem Biol 5:26–33
Flajsman M, Mandelc S, Radisek S et al (2016) Identification of novel virulence-associated proteins secreted to xylem by Verticillium nonalfalfae during colonization of hop plants. Mol Plant Microbe Interact 29(5):362–373
Gao W (2014) Analysis of protein changes using two-dimensional difference gel electrophoresis. Mol Toxicol Protocol 1105:17–30
Gomez-Gomez L, Boller T (2000) FLS2: an LRR receptor-like kinase involved in the perception of the bacterial elicitor flagellin in Arabidopsis. Mol Cell 5:1003–1011
Gonzalez-Fernandez R, Jorrin-Novo JV (2010) Proteomics of fungal plant pathogens: the case of Botrytis cinerea. In: Méndez-Vilas A (ed) Current research, technology and education topics in applied microbiology and microbial biotechnology. FORMATEX, Badajoz, pp 205–217
Gorg A, Weiss W, Dunn M (2004) Current two-dimensional electrophoresis technology for proteomics. Proteomics 4(12):3665–3685
Gourion B, Rossignol M, Vorholt JA (2006) A proteomic study of Methylobacterium extorquens reveals a response regulator essential for epiphytic growth. Proc Natl Acad Sci U S A 103:13186–13191
Grenville-Briggs LJ, Avrova AO, Bruce CR et al (2005) Elevated amino acid biosynthesis in Phytophthora infestans during appressorium formation and potato infection. Fungal Genet Biol 42:244–256
Guerreiro N, Redmond JW, Rolfe BG et al (1997) New Rhizobium leguminorum flavonoid induced proteins revealed by proteome analysis of differentially displayed proteins. Mol Plant-Microbe Interact 10:506–516
Gygi SP, Rist B, Gerber SA et al (1999) Quantitative analysis of complex protein mixtures using isotope-coded affinity tags. Nat Biotechnol 17(10):994–999
Hall DA, Zhu H, Zhu X et al (2004) Regulation of gene expression by a metabolic enzyme. Science 306:482–484
Hammond-Kosack KE, Jones JDG (2000) Responses to plant pathogens. In: Buchanan BB, Gruissem W, Jones RL (eds) Biochemistry and molecular biology of plants. American Society of Plant Physiology, Rockville, pp 1102–1156
Han X, Aslanian A, Yates JR (2008) Mass spectrometry for proteomics. Curr Opin C Biol 12(5):483–490
Hernandez LG, Lu B, Da Cruz GC et al (2012) Worker honeybee brain proteome. J Proteome Res 11:1485–1493
Hogenhout SA, Van der Hoorn RA, Terauchi R et al (2009) Emerging concepts in effector biology of plant-associated organisms. Mol Plant-Microbe Interact 22:115–122
Hoving S, Voshol H, Oostrum J (2000) Towards high performance two-dimensional gel electrophoresis using ultrazoom gels. Electrophoresis 21:2617–2621
Issaq HJ, Chan KC, Janini GM et al (2005) Multidimensional separation of peptides for effective proteomic analysis. J Chromatogr B 817(1):35–47
Jacobs JM, Babujee L, Meng F et al (2012) The in planta transcriptome of Ralstonia solanacearum: conserved physiological and virulence strategies during bacterial wilt of tomato. MBio 3:112–114
Jones JDG, Dangl JL (2006) The plant immune system. Nature 444:323–329
Jones AME, Thomas V, Truman B et al (2004) Specific changes in the Arabidopsis proteome in response to bacterial challenge: differentiating basal and R-gene mediated resistance. Phytochemistry 65:1805–1816
Jones AME, Thomas V, Bennett MH et al (2006) Modifications to the Arabidopsis defence proteome occur prior to significant transcriptional change in response to inoculation with Pseudomonas syringae. Plant Physiol 142:1603–1620
Kandasamy S, Loganathan K, Muthuraj R et al (2009) Understanding the molecular basis of plant growth promotional effect of Pseudomonas fluorescens on rice through protein profiling. Proteome Sci 7:47
Karas M, Hillenkamp F (1988) Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons. Anal Chem 60:2299–2301
Karunakaran R, Ramachandran VK, Seaman JC et al (2009) Transcriptomic analysis of Rhizobium leguminosarum biovar viciae in symbiosis with host plants Pisum sativum and Vicia cracca. J Bacteriol 191:4002–4014
Kazemi-Pour N, Condemine G, Hugouvieux-Cotte-Pattat N (2004) The secretome of the plant pathogenic bacterium Erwinia chrysanthemi. Proteomics 4:3177–3186
Kundu S, Chakraborty D, Pal A (2011) Proteomic analysis of salicylic acid induced resistance to Mungbean yellow mosaic India virus in Vigna mungo. J Proteome 74:337–349
Kwon YS, Lee DY, Rakwal R et al (2016) Proteomic analyses of the interaction between the plant-growth promoting Rhizobacterium paenibacillus polymyxa E681 and Arabidopsis thaliana. Proteomics 1:122–135
Larrainzar E, Wienkoop S, Weckwerth W et al (2007) Medicago truncatula root nodule proteome analysis reveals differential plant and bacteroid responses to drought stress. Plant Physiol 144:1495–1507
Larsen MKG, Jorgensen MM, Bennike TB et al (2016) Time-course investigation of Phytophthora infestans infection of potato leaf from three cultivars by quantitative proteomics. Elsevier Data Brief 6:238–248
Lee BJ, Kwon SJ, Kim SK et al (2006) Functional study of hot pepper 26S proteasome subunit RPN7 induced by tobacco mosaic virus from nuclear proteome analysis. Biochem Biophys Res Commun 351:405–411
Liang Y, Srivastava S, Rahman MH et al (2008) Proteome changes in leaves of Brassica napus L. as a result of Sclerotinia sclerotiorum challenge. J Agric Food Chem 56(60):1963–1976
Lo Presti L, Lanver D, Schweizer G et al (2015) Fungal effectors and plant susceptibility. Annu Rev Plant Biol 66:513–545
Lodha TD, Basak J (2012) Plant-pathogen interaction: what microarray tells about it? Mol Biotechnol 50(1):87–97
Lodha TD, Hembram P, Tep N et al (2013) Proteomics: a successful approach to understand the molecular mechanism of plant-pathogen interaction. Am J Plant Sci 4:1212–1226
Lund TC, Anderson LB, McCullar V (2007) iTRAQ is a useful method to screen for membrane-bound proteins differentially expressed in human natural killer cell types. J Proteome Res 6:644–653
Ma B (2015) Novor: real-time peptide de novo sequencing software. J Am Soc Mass Spectrom 26:1885–1894
Mahmood T, Jan A, Kakishima M et al (2006) Proteomic analysis of bacterial-blight defence responsive proteins in rice leaf blades. Proteomics 6:6053–6065
Mandelc S, Timperman I, Radisek S et al (2013) Comparative proteomic profiling in compatible and incompatible interactions between hop roots and Verticillium alboatrum. Plant Physiol Biochem 68:23–31
Maor R, Jones A, Nühse TS et al (2007) Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants. Mol Cell Proteomics 6(4):601–610
Marouga R, David S, Hawkins E (2005) The development of the DIGE system: 2D fluorescence difference gel analysis technology. Anal Bio Anal Chem 382(3):669–678
Martin GB, Bogdanove AJ, Sessa G (2003) Understanding the functions of plant disease resistance proteins. Annu Rev Plant Biol 54:23–61
Mathesius U, Mulders S, Gao M et al (2003) Extensive and specific responses of a eukaryote to bacterial quorum-sensing signals. Proc Natl Acad Sci U S A 100:1444–1449
Mattinen L, Nissinen R, Riipi T et al (2007) Host-extract induced changes in the secretome of the plant pathogenic bacterium Pectobacterium atrosepticum. Proteomics 7:3527–3537
McGregor E, Dunn MJ (2006) Proteomics of the heart unraveling disease. Circ Res 98(3):309–321
Mehta A, Rosato YB (2001) Differentially expressed proteins in the interaction of Xanthomonas axonopodis pv. citri with leaf extract of the host plant. Proteomics 1:1111–1118
Mehta A, Brasileiro ACM, Souza DSL et al (2008) Plant–pathogen interactions: what is proteomics telling us? FEBS J 275:3731–3746
Meijer HJ, Van-de-Vondervoort PJ, Yin QY et al (2006) Identification of cell wall-associated proteins from Phytophthora ramorum. Mol Plant-Microbe Interact 19:1348–1358
Moore CD, Ajala OZ, Zhu H (2016) Applications in high-content functional protein microarrays. Curr Opin Chem Biol 30:21–27
Mukherjee AK, Carp MJ, Zuchman R et al (2010) Proteomics of the response of Arabidopsis thaliana to infection with Alternaria brassicicola. J Proteome 73:709–720
Mur LA, Kenton P, Lloyd AJ et al (2008) The hypersensitive response; the centenary is upon us but how much do we know? J Expt Bot 59:501–520
Nat NVK, Srivastava S, Yajima W et al (2007) Application of proteomics to investigate plant-pathogen interactions. Curr Proteomics 4(1):28–43
Newton A, Fitt BDL, Atkins SD et al (2010) Pathogenesis, parasitism and mutualism in the trophic space of microbe–plant interactions. Trends Microbiol 18:365–373
Novak J, Lemr K, Schug KA et al (2015) CycloBranch: de novo sequencing of nonribosomal peptides from accurate product ion mass spectra. J Am Soc Mass Spectrom 10:1780–1786
Pakkianathan BC, Murad G (2014) Recent advances on interactions between the whitefly Bemisia tabaci and begomoviruses, with emphasis on Tomato yellow leaf curl virus. In: Gaur RK, Hohn T, Sharma P (eds) Plant virus-host interaction. Elsevier, Amsterdam, pp 79–103
Perez-Bueno ML, Rahoutei J, Sajnani C et al (2004) Proteomic analysis of the oxygen evolving complex of photosystem II under biotic stress: studies on Nicotiana benthamiana infected with tobamoviruses. Proteomics 4:418–425
Phalip V, Delalande F, Carapito C et al (2005) Diversity of the exoproteome of Fusarium graminearum grown on plant cell wall. Curr Genet 48:366–379
Pieterse CMJ, Van Loon LC (2004) NPR1: the spider in the web of induced resistance signaling pathways. Curr Opin Plant Biol 4:456–464
Rahoutei J, Baron M, Garcia-Luque I et al (1999) Effect of tobamovirus infection on the thermoluminescence characteristics of chloroplast from infected plants. Z Naturforsch Teil C 54:634–639
Rahoutei J, Garcia-Luque I, Baron M (2000) Inhibition of photosynthesis by viral infection: effect on PSII structure and function. Physiol Plant 110:286–292
Rampitsch C, Bykova NV, Mccallum B et al (2006) Analysis of the wheat and Puccinia triticina (leaf rust) proteomes during a susceptible host pathogen interaction. Proteomics 6:1897–1907
Righetti PG, Castagna A, Antonucci F et al (2004) Critical survey of quantitative proteomics in two-dimensional electrophoretic approaches. J Chromatogr A 1051:3–17
Rogowska-Wrzesinska A, Bihan MCL, Thaysen-Andersen M et al (2013) 2D gels still have a niche in proteomics. J Proteome 88:4–13
Romanov V, Davidoff SN, Miles AR et al (2014) A critical comparison of protein microarray fabrication technologies. Analyst 139(6):1303–1326
Rose JKC, Bashir S, Giovannoni JJ et al (2004) Tackling the plant proteome: practical approaches, hurdles and experimental tools. Plant J 5:715–733
Rosen R, Sacher A, Shechter N et al (2004) Two dimensional reference map of Agrobacterium tumefaciens proteins. Proteomics 4:1061–1073
Ross PL, Huang YN, Marchese JN et al (2004) Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents. Mol Cell Proteomics 3:1154–1169
Schenk PM, Choo JH, Wong CL (2009) Microarray analyses to study plant defense and rhizosphere microbe interaction. CAB Rev: Perspect Agric Vet Sci Nutr Nat Resour 45:1–14
Schwessinger B, Ronald PC (2012) Plant innate immunity: perception of conserved microbial signatures. Annu Rev Plant Biol 63:451–482
Smith R (2009) Two-dimensional electrophoresis: an overview. In: Tyther R, Sheehan D (eds) Two-dimensional electrophoresis protocols. Humana Press, Totowa, pp 2–17
Speer R, Wulfkuhle JD, Liotta LA et al (2005) 3rd reverse-phase protein microarrays for tissue-based analysis. Curr Opin Mol Ther 7:240–245
Speers AE, Wu CC (2007) Proteomics of integral membrane proteins theory and application. Chem Rev 107(8):3687–3714
Tanaka K, Waki H, Ido Y et al (1988) Protein and polymer analyses up to m/z 100,000 by laser ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 2:151–151
Tseng TT, Tyler BM, Setubal JC (2009) Protein secretion systems in bacterial-host associations, and their description in the gene ontology. BMC Microbiol 9:S2
Unlu M, Morgan ME, Minden JS (1997) Difference gel electrophoresis: a single gel method for detecting changes in protein extracts. Electrophoresis 18:2071–2077
Vadivel AK (2015) Gel-based proteomics in plants: time to move on from the tradition. Front Plant Sci 6:369
Valcu CM, Junqueira M, Shevchenko A (2009) Comparative proteomic analysis of responses to pathogen infection and wounding in Fagus sylvatica. J Proteome Res 8:4077–4091
Veenstra TD, Smith RD (eds) (2003) Proteome characterization and proteomics. Academic, San Diego
Ventelon-Debout M, Delalande F, Brizard JP et al (2004) Proteome analysis of cultivar-specific deregulations of Oryza sativa indica and O. sativa japonica cellular suspension undergoing rice yellow mottle virus infection. Proteomics 1:216–225
Wang L, Jiang W, Zhang Y et al (2013) Ax21-triggered immunity plays a significant role in rice defense against Xanthomonas oryzae pv. oryzicola. Phytopathology. https://doi.org/10.1094/PHYTO-12-12-0333-R
Washburn MP, Wolters D, Yates JR (2001) Large-scale analysis of the yeast proteome by multidimensional protein identification technology. Nat Biotechnol 19(3):242–247
Westermeier R (2006) Electrophoresis in practice. Wiley, Weinheim
White IR, Pickford R, Wood J et al (2004) Statistical comparison of silver and SYPRO ruby staining for proteomic analysis. Electrophoresis 17:3048–3054
Wilkins MR, Sanchez JC, Gooley AA et al (1996) Progress with proteome projects: why all proteins expressed by a genome should be identified and how to do it. Biotechnol Genet Eng Rev 13:19–50
Wittmann-Liebold H, Graack HR, Pohl T (2006) Two dimensional gel electrophoresis as tool for proteomics studies in combination with protein identification by mass spectrometry. Proteomics 17:4688–4703
Yajima W, Kav NN (2006) The proteome of the phytopathogenic fungus Sclerotinia sclerotiorum. Proteomics 6:5995–6007
Yang F, Melo-Braga MN, Larsen MR et al (2013) Battle through signaling between wheat and the fungal pathogen Septoria tritici revealed by proteomics and phosphoproteomics. Mol Cell Proteomics 12:2497–2508
Yang F, Li W, Derbyshire M et al (2015) Unraveling proteomics and Phosphoproteomics. Mol Cell Proteomics 12:2497–2508. incompatibility between wheat and the fungal pathogen Zymoseptoria tritici through apoplastic proteomics. BMC Genomics 16:362
Yates IJR, Gilchrist A, Howell KE et al (2005) Proteomics of organelles and large cellular structures. Nat Rev Mol Cell Biol 6(9):702–714
Zhou H, Ranish JA, Watts JD et al (2002) Quantitative proteome analysis by solid-phase isotope tagging and mass spectrometry. Nat Biotechnol 20(5):512–515
Zhou W, Eudes F, Laroche A (2006a) Identification of differentially regulated proteins in response to a compatible interaction between the pathogen Fusarium graminearum and its host, Triticum aestivum. Proteomics 6(16):4599–4609
Zhou W, Kolb FL, Riechers DE (2006b) Identification of proteins induced or upregulated by Fusarium head blight infection in the spikes of hexaploid wheat (Triticum aestivum). Genome 48(5):770–780
Zhu H, Bilgin M, Bangham R et al (2001) Global analysis of protein activities using proteome chips. Science 293:2101–2105
Zhu H, Bilgin M, Snyder M (2003) Proteomics. Annu Rev Biochem 1:783–812
Zhu W, Smith JW, Huang CM (2009) Mass spectrometry- based label-free quantitative proteomics. J Biomed Biotechnol 2010:Article ID: 840581
Zhu M, Simons B, Zhu N et al (2010) Analysis of abscisic acid responsive proteins in brassica Napus guard cells by multiplexed isobaric tagging. J Proteome 73(4):790–805
Zhu N, Zhu M, Dai S et al (2012) An improved isotope-coded affinity tag technology for thiol redox proteomics. J Integr OMICS 2(1):17–23
Zieske LR (2006) A perspective on the use of iTRAQ reagent technology for protein complex and profiling studies. J Exp Bot 57:1501–1508
Acknowledgement
The authors would like to acknowledge the kind help of Dr. Shalu Jain, Plant Pathology Division, North Dakota State University, Fargo, USA, for critical comments and suggestions.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Nature Singapore Pte Ltd.
About this chapter
Cite this chapter
Rustagi, A., Singh, G., Agrawal, S., Gupta, P.K. (2018). Proteomic Studies Revealing Enigma of Plant–Pathogen Interaction. In: Singh, A., Singh, I. (eds) Molecular Aspects of Plant-Pathogen Interaction. Springer, Singapore. https://doi.org/10.1007/978-981-10-7371-7_11
Download citation
DOI: https://doi.org/10.1007/978-981-10-7371-7_11
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-10-7370-0
Online ISBN: 978-981-10-7371-7
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)