Skip to main content

Sea Water as a Reaction Medium for Bioethanol Production

  • Chapter
  • First Online:
Microbial Biotechnology

Abstract

Lignocellulosic biorefineries are expected to combat the problems of depletion of fossil resources by large-scale production of chemicals and biofuels in future. Research studies in the past two decades have resulted in the systems for the production of bio-based novel products with improved ecological footprints. Among other challenges, the large scale operations would result in the huge consumption of fresh water (1.9–5.9 m3 water per m3 of biofuel) which will, in turn, question the sustainability of biorefineries. Fresh water shortage which already exists in many parts of our overpopulated planet has led to more uncertainties to biorefinery industries. Hence, some enzymatic and chemocatalytic applications using sea water as a reaction medium for large-scale biorefineries have been reported by several research groups, which emphasize the use of nonpotable water resources of coastal regions for locally available biomass. Many of those reports concluded that the use of such nonpotable water system is highly promising and hence the opportunities at the interface between biology and chemistry are predictable for holistic innovation and further research. The recent National Water Policy of India strongly advocated the implementation of new technology to minimize the fresh water consumption in industries. This critical situation warrants the design and development of economically, environmentally and socially sustainable practices for accomplishing sustainability in the global fine and speciality chemicals industry. The chapter has been planned to review the basics and research studies conducted to use the sea water as a reaction medium in bioethanol industries to reduce the usage of fresh water.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Aden A, Ruth M, Ibsen K, Jechura J, Neeves K, Sheehan J, Wallace B, Montague L, Slayton A, Lukas J (2002, June) Lignocellulosic biomass to ethanol process design and economics utilizing co-current dilute acid prehydrolysis and enzymatic hydrolysis for corn stover. Report No. NREL/TP-510-32438. Golden, CO: National Renewable Energy Laboratory. http://www.nrel.gov/docs/fy02osti/32438.pdf

  • Aden A, Spath P, Atherton A (2005, October) The potential of thermochemical ethanol via mixed alcohols production. Milestone completion report, FY05–684. National Renewable Energy Laboratory, Golden, CO

    Google Scholar 

  • Al-Hotti S, Kamel BS (1981) Utilization of sea water in vegetable fermentations. Enzym Microb Technol 3(4):353–356

    CAS  Google Scholar 

  • Aygan A, Karcioglu L, Arikan B (2011) Alkaline thermostable and halophilic endoglucanase from Bacillus licheniformis C108. Afr J Biotechnol 10(5):789–796

    CAS  Google Scholar 

  • Azadi H, de Jong S, Derudder B, De Maeyer P, Witlox F (2012) Bitter sweet: how sustainable is bio-ethanol production in Brazil? Renew Sust Energ Rev 16(6):3599–3603

    CAS  Google Scholar 

  • Behera S, Arora R, Nandhagopal N, Kumar S (2014) Importance of chemical pretreatment for bioconversion of lignocellulosic biomass. Renew Sust Energ Rev 36:91–106

    CAS  Google Scholar 

  • Biswas R, Uellendahl H, Ahring BK (2014) Wet explosion pretreatment of sugarcane bagasse for enhanced enzymatic hydrolysis. Biomass Bioenergy 61:104–113

    CAS  Google Scholar 

  • Bonugli-Santos RC, Durrant LR, Da Silva M, Sette LD (2010) Production of laccase, manganese peroxidase and lignin peroxidase by Brazilian marine-derived fungi. Enzym Microb Technol 46(1):32–37

    CAS  Google Scholar 

  • Bornscheuer UT, Pohl M (2001) Improved biocatalysts by directed evolution and rational protein design. Curr Opin Chem Biol 5(2):137–143

    CAS  PubMed  Google Scholar 

  • Bornscheuer U, Huisman G, Kazlauskas R, Lutz S, Moore J, Robins K (2012) Engineering the third wave of biocatalysis. Nature 485(7397):185–194. https://doi.org/10.1038/nature11117 PMID: 22575958

    Article  CAS  PubMed  Google Scholar 

  • Chiu CC, Shiang W-J, Lin CJ (2016) The water footprint of bioethanol. J Clean Energ Technol 4(1):43–47

    CAS  Google Scholar 

  • Dalby PA (2011) Strategy and success for the directed evolution of enzymes. Curr Opin Struct Biol 21(4):473–480

    CAS  PubMed  Google Scholar 

  • Dasilva R, Yim DK, Asquieri ER, Park YK (1993) Production of microbial alkaline cellulase and studies of their characteristics. Rev Microbiol 24:269–274

    CAS  Google Scholar 

  • Datta S, Holmes B, Park J, Chen Z, Dibble D, Hadi M, Blanch H, Simmons B, Sapra R (2010) Ionic liquid tolerant hyperthermophilic cellulases for biomass pretreatment and hydrolysis. Green Chem 12:338–345

    CAS  Google Scholar 

  • Demirjian DC, Morís-Varas F, Cassidy CS (2001) Enzymes from extremophiles. Curr Opin Chem Biol 5(2):144–151

    CAS  PubMed  Google Scholar 

  • Domínguez de María P (2013) On the use of seawater as reaction media for large-scale applications in biorefineries. Chem Cat Chem 5(7):1643–1648

    Google Scholar 

  • Earth Policy Institute (2012) Full planet, empty plates. Chapter 4 data: food or fuel? (online). Available: http://www.earth-policy.org/data_center/C23. Accessed 25 July 2017

  • EISA (Energy Independence and Security Act) (2007) PUBLIC LAW 110–140—DEC. 19, 2007; 121 STAT. 1492–1801. Energy Independence and Security Act of 2007. 42 USC 17001 note

    Google Scholar 

  • Engel P, Mladenov R, Wulfhorst H, Jager G, Spiess AC (2010) Point by point analysis: how ionic liquid affects the enzymatic hydrolysis of native and modified cellulose. Green Chem 12:1959–1966

    CAS  Google Scholar 

  • Esclapez J, Pire C, Bautista V, Martínez-Espinosa RM, Ferrer J, Bonete MJ (2007) Analysis of acidic surface of Haloferax mediterranei glucose dehydrogenase by site-directed mutagenesis. FEBS Lett 581(5):837–842

    CAS  PubMed  Google Scholar 

  • Fang C, Thomsen MH, Brudecki GP, Cybulska I, Frankaer CG, Oyanedel JRB, Schmidt JE (2015) Seawater as an alternative to freshwater in the pretreatment of date palm residues for bioethanol production in coastal and/or arid areas. Chem Sus Chem 8(22):3823–3831

    CAS  Google Scholar 

  • Gachomo WE (2003) Bacillus pumilus BPCRI 6, a promising candidate for cellulase production under conditions catabolite repression. Afr J Biotechnol 2:140–146

    Google Scholar 

  • Garg R, Srivastava R, Brahma V, Verma L, Karthikeyan S, Sahni G (2016) Biochemical and structural characterization of a novel halotolerant cellulase from soil metagenome. Sci Rep 23(6):39634

    Google Scholar 

  • Gerbens-Leenes PW, Van Lienden AR, Hoekstra AY, Van der Meer TH (2012) Biofuel scenarios in a water perspective: the global blue and green water footprint of road transport in 2030. Glob Environ Chang 22(3):764–775

    Google Scholar 

  • Gonçalves FA, Santos ESD, de Macedo GR (2015) Alcoholic fermentation of Saccharomyces cerevisiae, Pichia stipitis and Zymomonas mobilis in the presence of inhibitory compounds and seawater. J Basic Microbiol 55(6):695–708

    PubMed  Google Scholar 

  • Grande PM, De Maria PD (2012) Enzymatic hydrolysis of microcrystalline cellulose in concentrated seawater. Bioresour Technol 104:799–802

    CAS  PubMed  Google Scholar 

  • Grande PM, Bergs C, Domínguez de María P (2012) Chemo-enzymatic conversion of glucose into 5-hydroxymethylfurfural in seawater. Chem Sus Chem 5(7):1203–1206

    CAS  Google Scholar 

  • Grant S, Sorokin DY, Grant WD, Jones BE, Heaphy S (2004) A phylogenetic analysis of Wadi el Natrun soda lake cellulase enrichment cultures and identification of cellulase genes from these cultures. Extremophiles 8(5):421–429

    CAS  PubMed  Google Scholar 

  • Gunny AAN, Arbain D (2013) Ionic liquids: green solvent for pretreatment of lingnocellulosic biomass. Adv Mater Res 701:399–402

    Google Scholar 

  • Hakobyan A, Panosyan H, Trchounian A (2013) Production of cellulase by the haloalkalophilic strains of Streptomyces isolated from saline-alkaline soils of Ararat plain, Armenia. Electron J Nat Sci 1:21(2)

    Google Scholar 

  • Hirasawa K, Uchimura K, Kashiwa M, Grant W, Ito S, Kobayashi T, Horikoshi K (2006) Salt-activated endoglucanase of a strain of alkaliphilic Bacillus agaradhaerens. Antonie Van Leeuwenhoek 89:211–219

    CAS  PubMed  Google Scholar 

  • Hoekstra (2002) Virtual water trate in Proc. The International Expert Meeting on virtual water Trade, Delft, Netherlands

    Google Scholar 

  • Hoekstra AK, Chapagain MM, Aldaya M, Mekonnen M (2011) The water footprint assessment manual: setting the global standard. Earthscan, London

    Google Scholar 

  • Holland RA, Scott KA, Florke M et al (2015) Global impacts of energy demand on the freshwater resources of nations. PNAS:E6707–E6716

    Google Scholar 

  • Holt JG, Krieg NR, Sneath PHA, Staley JT (1994) Bergey’s manual of determinative bacteriology/ninth edition. Williams &Willkins, Baltimore, p 547

    Google Scholar 

  • Ilmberger N, Meske D, Juergensen J, Schulte M, Barthen P, Rabausch U, Angelov A, Mientus M, Liebl W, Schmitz RA, Streit WR (2012) Metagenomic cellulases highly tolerant towards the presence of ionic liquid slinking thermostability and halotolerance. Appl Microbiol Biotechnol 95:135–146

    CAS  PubMed  Google Scholar 

  • Indira D, Sharmila D, Balasubramanian P, Thirugnanam A, Jayabalan R (2016) Utilization of sea water based media for the production and characterization of cellulase by Fusarium subglutinans MTCC 11891. Biocat Agric Biotechnol 7:187–192

    Google Scholar 

  • Institute for Agriculture and Trade Policy (IATP) (2006) Water use by ethanol plants potential challenges, Minneapolis, MN. www.agobservatory.org/library.cfm?refid=89449

  • Jeyanthi GP, Subramanian J (2011) A comparison between microwave assisted alkaline sodium hydroxide and alkaline hydrogen peroxide pretreatments of green coconut fiber for bioethanol production. Asian J Microbiol Biotechnol Environ Sci 13(2):365–369

    CAS  Google Scholar 

  • Jo KI, Lee YJ, Kim BK, Lee BH, Jung CH, Nam SW (2008) Pilot-scale production of carboxymethylcellulase from rice hull by Bacillus amyloliquefaciens L-3. Biotechnol Bioproc Eng 13:182–188

    CAS  Google Scholar 

  • Kamiya N, Matsushita Y, Hanaki M, Nakashima K, Narita M, Goto M, Takahashi H (2008) Enzymatic in situ saccharification of cellulose in aqueous ionic liquid media. Biotechnol Lett 30:1037–1040

    CAS  PubMed  Google Scholar 

  • Karan R, Capes MD, Dassarma S (2012) Function and biotechnology of extremophilic enzymes in low water activity. Aquat Biosyst 8(1):4. https://doi.org/10.1186/2046-9063 8–4

    CAS  PubMed  PubMed Central  Google Scholar 

  • Kaur K, Phutela UG (2016) Enhancement of paddy straw digestibility and biogas production by sodium hydroxide-microwave pretreatment. Renew Energy 92:178–184

    CAS  Google Scholar 

  • Khandeparker R, Verma P, Deobagkar D (2011) A novel halotolerant xylanase from marine isolate Bacillus subtilis cho40: gene cloning and sequencing. New Biotechnol 28(6):814–821

    CAS  Google Scholar 

  • Kim JW, Peeples TL (2006) Screening extremophiles for bioconversion potentials. Biotechnol Prog 22(6):1720–1724

    CAS  PubMed  Google Scholar 

  • Klement T, Milker S, Jäger G, Grande PM, de María PD, Büchs J (2012) Biomass pretreatment affects Ustilago maydis in producing itaconic acid. Microb Cell Factories 11(1):43

    CAS  Google Scholar 

  • Ko CH, Chen WL, Tsai CH, Jane WN, Liu CC, Tu J (2007) Paenibacillus campinasensis BL11: a wood material-utilizing bacterial strain isolated from black liquor. Bioresour Technol 98:2727–2733

    CAS  PubMed  Google Scholar 

  • Komives CF, Cheung LYY, Pluschkell SB, Flickinger MC (2005) Growth of Bacillus methanolicus in seawater-based media. J Ind Microbiol Biotechnol 32(2):61–66

    CAS  PubMed  Google Scholar 

  • Kuhad RC, Gupta R, Singh A (2011) Microbial cellulases and their industrial applications. Enzym Res 2011:280696

    Google Scholar 

  • Kumar P, Barrett DM, Delwiche MJ, Stroeve P (2009) Methods for pretreatment of lignocellulosic biomass for efficient hydrolysis and biofuel production. Ind Eng Chem Res 48(8):3713–3729

    CAS  Google Scholar 

  • Lee YJ, Kim BK, Lee BH, Jo KI, Lee NK, Chung CH, Lee YC, Lee JW (2008) Purification and characterization of cellulase produced by Bacillus amyoliquefaciens DL-3 utilizing rice hull. Bioresour Technol 99(2):378–386

    CAS  PubMed  Google Scholar 

  • Lehmann C, Sibilla F, Maugeri Z, Streit WR, de María PD, Martinez R, Schwaneberg U (2012) Reengineering CelA2 cellulase for hydrolysis in aqueous solutions of deep eutectic solvents and concentrated seawater. Green Chem 14(10):2719–2726

    CAS  Google Scholar 

  • Li X, Yu HY (2012) Purification and characterization of an organic-solvent-tolerant cellulase from a halotolerant isolate, Bacillus sp. L1. J Ind Microbiol Biotechnol 39(8):1117–1124

    CAS  PubMed  Google Scholar 

  • Li X, Yu HY (2013) Characterization of a halostable endoglucanase with organic solvent-tolerant property from Haloarcula sp. G10. Int J Biol Macromol 30(62):101–106

    CAS  Google Scholar 

  • Liang C, Fioroni M, Rodríguez-Ropero F, Xue Y, Schwaneberg U, Ma Y (2011) Directed evolution of a thermophilic endoglucanase (Cel5A) into highly active Cel5A variants with an expanded temperature profile. J Biotechnol 154(1):46–53

    CAS  PubMed  Google Scholar 

  • Lin CS, Luque R, Clark JH, Webb C, Du C (2011) A seawater based biorefining strategy for fermentative production and chemical transformations of succinic acid. Energy Environ Sci 4(4):1471–1479

    CAS  Google Scholar 

  • Lorenz P, Liebeton K, Niehaus F, Eck J (2002) Screening for novel enzymes for biocatalytic processes: accessing the metagenome as a resource of novel functional sequence space. Curr Opin Biotechnol 13(6):572–577

    CAS  PubMed  Google Scholar 

  • Lynd LR, Laser MS, Bransby D, Dale BE, Davison B, Hamilton R, Wyman CE (2008) How biotech can transform biofuels. Nat Biotechnol 26(2):169

    CAS  PubMed  Google Scholar 

  • Lynd LR, Larson E, Greene N, Laser M, Sheehan J, Dale BE, Wang M (2009) The role of biomass in America’s energy future: framing the analysis. Biofuels Bioprod Bioref 3(2):113–123

    CAS  Google Scholar 

  • Madern D, Pfister C, Zaccai G (1995) Mutation at a single acidic amino acid enhances the halophilic behaviour of malate dehydrogenase from Haloarcula marismortui in physiological salts. Eur J Biochem 230:1088–1095

    CAS  PubMed  Google Scholar 

  • Maki-Arvela P, Anugwom I, Virtanen P, Sjoholm R, Mikkola JP (2010) Dissolution of lignocellulosic materials and its constituents using ionic liquids – a review. Ind Crop Prod 32:175–201

    Google Scholar 

  • Mayende L, Wilhelmi BS, Pletschke BI (2006) Cellulases (CMCases) and polyphenol oxidases from thermophilic Bacillus sp. isolated from compost. Soil Biol Biochem 38:2963–2966

    CAS  Google Scholar 

  • Mesbah NM, Wiegel J (2005) Halophilic thermophiles: a novel group of extremophiles. In: Satyanarayana T, Johri BN (eds) Microbial diversity: current perspectives and potential applications. I.K. Publishing House, New Delhi, pp 91–118

    Google Scholar 

  • Mormeneo M, Pastor FJ, Zueco J (2012) Efficient expression of a Paenibacillus barcinonensis endoglucanase in Saccharomyces cerevisiae. J Ind Microbiol Biotechnol 39:115–123

    CAS  PubMed  Google Scholar 

  • Oren A (2006) Life at high salt concentrations. In: Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E (eds) The prokaryotes. Springer, New York, pp 263–282

    Google Scholar 

  • Oren A (2010) Industrial and environmental applications of halophilic microorganisms. Environ Technol 31:825–834

    CAS  PubMed  Google Scholar 

  • Owens S (2007, June) Reduce cooling tower water consumption by 20%, Ethanol Producer Magazine. http://www.ethanolproducer.com, www.ethanolproducer.com

  • Pason P, Kyu KL, Ratanakhanokchai K (2006) Paenibacillus curdlanolyticus strain B-6 xylanolytic–cellulolytic enzyme system that degrades insoluble polysaccharides. Appl Environ Microbiol 72:2483–2490

    CAS  PubMed  PubMed Central  Google Scholar 

  • Percival ZYH, Himmel ME, Mielenz JR (2006) Outlook for cellulase improvement: screening and selection strategies. Biotechnol Adv 24:452–481

    Google Scholar 

  • Perlack RD et al (2005) Biomass as feedstock for a bioenergy and bioproducts industry: the technical feasibility of a billion-ton annual supply. Oak Ridge National Lab TN, Oak Ridge

    Google Scholar 

  • Pimentel D, Marklein A, Toth MA, Karpoff M, Paul GS, McCormack R, Krueger T (2008) Biofuel impacts on world food supply: use of fossil fuel, land and water resources. Energies 1(2):41–78

    Google Scholar 

  • Pottkämper J, Barthen P, Ilmberger N, Schwaneberg U, Schenk A, Schulte M, Streit WR (2009) Applying metagenomics for the identification of bacterial cellulases that are stable in ionic liquids. Green Chem 11(7):957–965

    Google Scholar 

  • Raddadi N, Cherif A, Daffonchio D, Fava F (2013) Halo-alkalitolerant and thermostable cellulases with improved tolerance to ionic liquids and organic solvents from Paenibacillus tarimensis isolated from the Chott El Fejej, Sahara desert, Tunisia. Bioresour Technol 31(150):121–128

    Google Scholar 

  • Ren H, Zong MH, Wu H, Li N (2016) Utilization of seawater for the biorefinery of lignocellulosic biomass: ionic liquid pretreatment, enzymatic hydrolysis, and microbial lipid production. ACS Sust Chem Eng 4(10):5659–5666

    CAS  Google Scholar 

  • Romero A, Santos A, Tojo J, Rodriguez A (2008) Toxicity and biodegradability of imidazolium ionic liquids. J Hazard Mater 151(1):268–273

    CAS  PubMed  Google Scholar 

  • Sabu A, Keerthi TR, Kumar SR, Chandrasekaran M (2000) L-Glutaminase production by marine Beauveria sp. under solid state fermentation. Process Biochem 35(7):705–710

    CAS  Google Scholar 

  • Salvador AC, Santos MC, Saraiva JA (2010) Effect of ionic liquid and high pressure on the activity of cellulase. Green Chem 12:632–635

    CAS  Google Scholar 

  • Samayam IP, Schall CA (2010) Saccharification of ionic liquid pretreated biomass with commercial enzyme mixtures. Bioresour Technol 101(10):3561–3566

    CAS  PubMed  Google Scholar 

  • Senthilraja P, Kathiresan K, Saravanakumar K (2011) Comparative analysis of bioethanol production by different strains of immobilized marine yeast. J Yeast Fungal Res 2(8):113–116

    CAS  Google Scholar 

  • Sharma S, Kumar R, Gaur R, Agrawal R, Gupta RP, Tuli DK, Das B (2015) Pilot scale study on steam explosion and mass balance for higher sugar recovery from rice straw. Bioresour Technol 175:350–357

    CAS  PubMed  Google Scholar 

  • Shinoda S, Kanamasa S, Arai M (2012) Improved reaction pattern of an endoglycanase from Paenibacillus cookii for chitosan oligosaccharide production. Carbohydr Res 359:54–58

    CAS  PubMed  Google Scholar 

  • Silalertruksa T, Gheewala SH (2009) Environmental sustainability assessment of bio-ethanol production in Thailand. Energy 34:1933–1946

    CAS  Google Scholar 

  • Sindhu R, Kuttiraja M, Binod P, Sukumaran RK, Pandey A (2014) Bioethanol production from dilute acid pretreated Indian bamboo variety (Dendrocalamus sp.) by separate hydrolysis and fermentation. Ind Crop Prod 52:169–176

    CAS  Google Scholar 

  • Singh VK, Kumar A (1998) Production and purification of an extracellular cellulase from Bacillus brevis VS-1. Biochem Mol Biol Int 45:443–452

    CAS  PubMed  Google Scholar 

  • Singh A, Tuteja S, Singh N, Bishnoi NR (2011) Enhanced saccharification of rice straw and hull by microwave–alkali pretreatment and lignocellulolytic enzyme production. Bioresour Technol 102(2):1773–1782

    CAS  PubMed  Google Scholar 

  • Singh R, Shukla A, Tiwari S, Srivastava M (2014a) A review on delignification of lignocellulosic biomass for enhancement of ethanol production potential. Renew Sust Energ Rev 32:713–728

    CAS  Google Scholar 

  • Singh R, Tiwari S, Srivastava M, Shukla A (2014b) Experimental study on the performance of microwave assisted Hydrogen peroxide (H2O2) pretreatment of rice straw. Agric Eng Int CIGR J 16(1):173–181

    Google Scholar 

  • Singh J, Suhag M, Dhaka A (2015a) Augmented digestion of lignocellulose by steam explosion, acid and alkaline pretreatment methods: a review. Carbohydr Polym 117:624–631

    CAS  PubMed  Google Scholar 

  • Singh S, Cheng G, Sathitsuksanoh N, Wu D, Varanasi P, George A, Wyman CE (2015b) Comparison of different biomass pretreatment techniques and their impact on chemistry and structure. Fr Energ Res 2:62

    Google Scholar 

  • Subhedar PB, Babu NR, Gogate PR (2015) Intensification of enzymatic hydrolysis of waste newspaper using ultrasound for fermentable sugar production. Ultrason Sonochem 22:326–332

    CAS  PubMed  Google Scholar 

  • Sukumaran RK, Singhania RR, Pandey A (2005) Microbial cellulases-production, applications and challenges. J Sci Ind Res 64:832–844

    CAS  Google Scholar 

  • Trivedi N, Gupta V, Kumar M, Kumari P, Reddy CRK, Jha B (2010) An alkali-halotolerant cellulase from Bacillus flexus isolated from green seaweed Ulva lactuca. Carbohydr Polym 83(2):891–897

    Google Scholar 

  • Trivedi N, Gupta V, Reddy CRK, Jha B (2013) Detection of ionic liquid stable cellulase produced by the marine bacterium Pseudoalteromonas sp. isolated from brown alga Sargassum polycystum C. Agardh. Bioresour Technol 132:313–319

    CAS  PubMed  Google Scholar 

  • Turner MB, Spear SK, Huddleston JG, Holbrey JD, Rogers RD (2003) Ionic liquid salt-induced inactivation and unfolding of cellulase from Trichoderma reesei. Green Chem 5:443–444

    CAS  Google Scholar 

  • Urano N, Yamazaki M, Ueno R (2001) Distribution of halotolerant and/or fermentative yeasts in aquatic environments. Journal-Tokyo University of Fisheries 87:23–30

    Google Scholar 

  • Vom Stein T, Grande P, Sibilla F, Commandeur U, Fischer R, Leitner W, de María PD (2010) Salt-assisted organic-acid-catalyzed depolymerization of cellulose. Green Chem 12(10):1844–1849

    CAS  Google Scholar 

  • Wallner T, Mike D (2011). Alternative fuels and propulsion systems – A U.S. perspective (online). Available: http://www.transportation.anl.gov/pdfs/AF/734.PDF. Accessed on 25 July 2017

  • Wejse PL, Ingvorsen K, Mortensen KK (2003) Purification and characterisation of two extremely halotolerant xylanases from a novel halophilic bacterium. Extremophiles 7(5):423–431

    CAS  PubMed  Google Scholar 

  • Werpy T, Petersen G, Aden A, Bozell J, Holladay J, White J, Jones S (2004) Top value added chemicals from biomass, Volume 1-Results of screening for potential candidates from sugars and synthesis gas (No. DOE/GO-102004-1992). Department of Energy, Washington, DC

    Google Scholar 

  • Wu M, Mintz M, Wang M, Arora S (2009) Water consumption in the production of ethanol and petroleum gasoline. Environ Manag 44(5):981–997

    Google Scholar 

  • WWAP (World Water Assessment Programme) (2012) The United Nations world water development report 4: managing water under uncertainty and risk. UNESCO, Paris

    Google Scholar 

  • Xing M-N, Zhang X-Z, Huang H (2012) Application of metagenomic techniques in mining enzymes from microbial communities for biofuel synthesis. Biotechnol Adv 30:920–929

    CAS  PubMed  Google Scholar 

  • Yin LJ, Lin HH, Xiao ZR (2010) Purification and characterization of a cellulase from Bacillus subtilis YJ1. J Mar Sci Technol 18:466–471

    Google Scholar 

  • Zarafeta D, Kissas D, Sayer C, Gudbergsdottir SR, Ladoukakis E, Isupov MN, Chatziioannou A, Peng X, Littlechild JA, Skretas G, Kolisis FN (2016) Discovery and characterization of a thermostable and highly halotolerant GH5 cellulase from an icelandic hot spring isolate. PLoS One 7;11(1):e0146454

    Google Scholar 

  • Zhang J, Ma X, Yu J, Zhang X, Tan T (2011) The effects of four different pretreatments on enzymatic hydrolysis of sweet sorghum bagasse. Bioresour Technol 102(6):4585–4589

    CAS  PubMed  Google Scholar 

  • Zhang G, Li S, Xue Y, Mao L, Ma Y (2012) Effects of salts on activity of halophilic cellulase with glucomannanase activity isolated from alkaliphilic and halophilic Bacillus sp. BG-CS10. Extremophiles 16:35–43

    CAS  PubMed  Google Scholar 

  • Zhao H, Jones CL, Baker GA, Xia S, Olubajo O, Person VN (2009) Regenerating cellulose from ionic liquids for an accelerated enzymatic hydrolysis. J Biotechnol 139:47–54

    CAS  PubMed  Google Scholar 

  • Zhao K, Guo LZ, Lu WD (2012) Extracellular production of novel halotolerant, thermostable, and alkali-stable carboxymethyl cellulase by marine bacterium Marinimicrobium sp. LS-A18. Appl Biochem Biotechnol 1:1–8

    Google Scholar 

  • Zhou Y, Wang X, Wei W, Xu J, Wang W, Xie Z, Zhang Z, Jiang H, Wang Q, Wei C (2016) A novel efficient β-glucanase from a paddy soil microbial metagenome with versatile activities. Biotechnol Biofuels 9(1):36

    PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgement

Authors acknowledge the PhD fellowship given by NIT Rourkela, MHRD, Government of India.

Conflict of Interest Statement

The authors declare that they have no conflict of interest disclosed in this work.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to R. Jayabalan .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Nature Singapore Pte Ltd.

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Indira, D., Das, B., Balasubramanian, P., Jayabalan, R. (2018). Sea Water as a Reaction Medium for Bioethanol Production. In: Patra, J., Das, G., Shin, HS. (eds) Microbial Biotechnology. Springer, Singapore. https://doi.org/10.1007/978-981-10-7140-9_9

Download citation

Publish with us

Policies and ethics