Membrane Proteins

Chapter

Abstract

Proteins associated with membranes include the integral membrane proteins, which are embedded in the membrane, and the easily extractable peripheral proteins which loosely adhere to the membrane surface by noncovalent interactions either with membrane lipids or with other membrane proteins. The domains of integral membrane proteins traversing the membrane are either α helices or multiple β strands. Another category of proteins is the lipid-anchored proteins, which are associated with the membrane by covalent attachment to lipids embedded in the membrane. Though membrane proteins represent around one-third of the proteomes of most organisms, their solubilization, purification, and crystallization still remain a challenge. The native environment of membrane proteins is the hydrophobic lipid bilayer. Hence, a major challenge of membrane protein purification is to obtain the protein in its native conformation in aqueous solution. Amphiphilic detergents have been used as invaluable tools to solubilize, isolate, and characterize membrane proteins. Membrane proteins are asymmetrically oriented in membranes. Many tools are now available to study the topology of membrane proteins.

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Copyright information

© Springer Nature Singapore Pte Ltd. 2017

Authors and Affiliations

  1. 1.Department of BiochemistryShivaji CollegeNew DelhiIndia
  2. 2.Department of BiochemistryDaulat Ram College, University of DelhiNew DelhiIndia

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