Membrane Proteins

  • Rashmi Wardhan
  • Padmshree Mudgal


Proteins associated with membranes include the integral membrane proteins, which are embedded in the membrane, and the easily extractable peripheral proteins which loosely adhere to the membrane surface by noncovalent interactions either with membrane lipids or with other membrane proteins. The domains of integral membrane proteins traversing the membrane are either α helices or multiple β strands. Another category of proteins is the lipid-anchored proteins, which are associated with the membrane by covalent attachment to lipids embedded in the membrane. Though membrane proteins represent around one-third of the proteomes of most organisms, their solubilization, purification, and crystallization still remain a challenge. The native environment of membrane proteins is the hydrophobic lipid bilayer. Hence, a major challenge of membrane protein purification is to obtain the protein in its native conformation in aqueous solution. Amphiphilic detergents have been used as invaluable tools to solubilize, isolate, and characterize membrane proteins. Membrane proteins are asymmetrically oriented in membranes. Many tools are now available to study the topology of membrane proteins.


  1. Almén MS, Nordström KJ, Fredriksson R, Schiöth HB (2009) Mapping the human membrane proteome: a majority of the human membrane proteins can be classified according to function and evolutionary origin. BMC Biol 7:50. (PMC 2739160. PMID 19678920)CrossRefPubMedPubMedCentralGoogle Scholar
  2. Caffrey M (2003) Membrane protein crystallization. J Struct Biol 142:108–132CrossRefPubMedGoogle Scholar
  3. Deisenhofer J, Epp O, Miki K, Huber R, Michel H (1985) Structure of the protein subunits in the photosynthetic reaction centre of rhodopseudomonas viridis at 3 A resolution. Nature 318:618–624. CrossRefPubMedGoogle Scholar
  4. Findlay JBC, Evans WH (1987) Biological membranes: a practical approach. IRL PressGoogle Scholar
  5. Gophna U, Ideses D, Rosen R et al (2004) OmpA of a septicemic Escherichia coli O78—secretion and convergent evolution. Int J Med Microbiol 294:373–381CrossRefPubMedGoogle Scholar
  6. Hurwitz N, Pellegrini-Calace M, Jones DT (2006) Towards genome-scale structure prediction for transmembrane proteins. Phil Trans R Soc B 361:465–475CrossRefPubMedPubMedCentralGoogle Scholar
  7. Kenrew JC,  Bodo G, Dintzis HM,  Parrish RG,  Wyckoff H,  Phillips DC (1958) A three-dimensional model of the myoglobin molecule obtained by X-ray analysis, Nature  181:662–666Google Scholar
  8. Kobilka BK (2007) G protein coupled receptor structure and activation. Biochimica et iophysica acta 1768(4):794–807. CrossRefGoogle Scholar
  9. Kyte J, Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157:110CrossRefGoogle Scholar
  10. Landau EM, Rosenbusch JP (1996) Lipidic cubic phases: A novel concept for the crystallization of membrane proteins. Proceedings of the National Academy of Sciences 93(25):14532–14535Google Scholar
  11. Lefkowitz RJ, Kobilka BK (2012) The nobel prize in chemistry. Royal Swed Acad SciGoogle Scholar
  12. MARCKS_HUMAN (2007) Swiss-Prot.
  13. Ott CM, Lingappa VR (2002) Integral membrane protein biosynthesis: why topology is hard to predict. J Cell Sci 115:2003–2009PubMedGoogle Scholar
  14. Pautsch A, Schulz GE (1998) Structure of the outer membrane protein A transmembrane domain. Nat Struct Biol 5:1013–1017CrossRefPubMedGoogle Scholar
  15. Perutz MF, Rossmann MG, Ann F, Cullis AF, Muirhead H, Will G, North ACT (1960) Structure of haemoglobin: A three-dimensional Fourier synthesis at 5.5- ̊ A resolution obtained by X-ray analysis, Nature  185:416–422Google Scholar
  16. Power ML, Ferrari BC, Littlefield-Wyer J, Gordon DM, Slade MB, Veal DA (2006) A naturally occurring novel allele of Escherichia coli outer membrane protein A reduces sensitivity to bacteriophage. Appl Environ Microbiol 72:7930–7932Google Scholar
  17. Singer SJ, Nicolson GL (1972) The Fluid Mosaic Model of the Structure of Cell Membranes. Science 175 (4023):720–731Google Scholar
  18. Skach WR (2009) Cellular mechanisms of membrane protein folding. Nat Struct Mol Biol 16:606–612. CrossRefPubMedPubMedCentralGoogle Scholar
  19. Smith SO (2012) Insights into the activation mechanism of the visual receptor rhodopsin. Biochm Soc Trans 40:389–393CrossRefGoogle Scholar
  20. Stahelin RV (2009) Lipid binding domains: more than simple lipid effectors. J Lipid Res 50:S299–S304CrossRefPubMedPubMedCentralGoogle Scholar
  21. Taylor DR, Hooper NM (2011) GPI-anchored proteins in health and disease. In: Vidal CJ (ed) Post-translational modifications in health and disease, protein reviews, vol 13. Springer Science + Business Media, LLCGoogle Scholar

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© Springer Nature Singapore Pte Ltd. 2017

Authors and Affiliations

  1. 1.Department of BiochemistryShivaji CollegeNew DelhiIndia
  2. 2.Department of BiochemistryDaulat Ram College, University of DelhiNew DelhiIndia

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