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Molecular Mechanism for Chromatin Regulation During MCM Loading in Mammalian Cells

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Book cover DNA Replication

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1042))

Abstract

DNA replication is a fundamental process required for the accurate and timely duplication of chromosomes. During late mitosis to G1 phase, the MCM2-7 complex is loaded onto chromatin in a manner dependent on ORC, CDC6, and Cdt1, and chromatin becomes licensed for replication. Although every eukaryotic organism shares common features in replication control, there are also some differences among species. For example, in higher eukaryotic cells including human cells, no strict sequence specificity has been observed for replication origins, unlike budding yeast or bacterial replication origins. Therefore, elements other than beyond DNA sequences are important for regulating replication. For example, the stability and precise positioning of nucleosomes affects replication control. However, little is known about how nucleosome structure is regulated when replication licensing occurs. During the last decade, histone acetylation enzyme HBO1, chromatin remodeler SNF2H, and histone chaperone GRWD1 have been identified as chromatin-handling factors involved in the promotion of replication licensing. In this review, we discuss how the rearrangement of nucleosome formation by these factors affects replication licensing.

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Acknowledgment

We thank all members of our research group for the helpful discussion and critical reading of the manuscript. This work was supported in part by Grants to Sugimoto and Fujita from the Ministry of Education, Culture, Sports, Science and Technology of Japan (17080013, 21370084, 25291027, 26114713, 09J07233).

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The authors declare no conflicts of interest in relation to this article.

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  1. Department of Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan

    Nozomi Sugimoto & Masatoshi Fujita

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  1. Nozomi Sugimoto
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  2. Masatoshi Fujita
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Correspondence to Nozomi Sugimoto or Masatoshi Fujita .

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  1. Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan

    Hisao Masai

  2. Istituto FIRC di Oncologia Molecolare, Milan, Italy

    Marco Foiani

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Sugimoto, N., Fujita, M. (2017). Molecular Mechanism for Chromatin Regulation During MCM Loading in Mammalian Cells. In: Masai, H., Foiani, M. (eds) DNA Replication. Advances in Experimental Medicine and Biology, vol 1042. Springer, Singapore. https://doi.org/10.1007/978-981-10-6955-0_3

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