The Temporal Regulation of S Phase Proteins During G1

  • Gavin D. Grant
  • Jeanette G. CookEmail author
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1042)


Successful DNA replication requires intimate coordination with cell-cycle progression. Prior to DNA replication initiation in S phase, a series of essential preparatory events in G1 phase ensures timely, complete, and precise genome duplication. Among the essential molecular processes are regulated transcriptional upregulation of genes that encode replication proteins, appropriate post-transcriptional control of replication factor abundance and activity, and assembly of DNA-loaded protein complexes to license replication origins. In this chapter we describe these critical G1 events necessary for DNA replication and their regulation in the context of both cell-cycle entry and cell-cycle progression.


Cell cycle Origin licensing RB E2F Cyclin CDK APC/C Quiescence DREAM complex 



We thank members of the Cook lab for feedback and discussion about the manuscript and DBG for critical reading of the manuscript. This work was supported by funding to G.D.G. from the ITCMS training grant (T32CA009156) and to J.G.C. from the National Institutes of Health NIGMS (R01GM102413) and the W.M. Keck Foundation.

Conflicts of Interest

The authors have no conflicts of interest.


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© Springer Nature Singapore Pte Ltd. 2017

Authors and Affiliations

  1. 1.Department of Biochemistry and BiophysicsUniversity of North Carolina at Chapel HillChapel HillUSA
  2. 2.Lineberger Comprehensive Cancer CenterUniversity of North Carolina at Chapel HillChapel HillUSA

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