Rif1-Dependent Regulation of Genome Replication in Mammals
Eukaryotic genomes are replicated starting from multiple origins of replication. Their usage is tightly regulated, and not all the potential origins are activated during a single cell cycle. In addition, the ones that are activated are activated in a sequential order. Why don’t origins of replication normally all fire together? Is this important? And if so, why? Would any order of firing do, or does the specific sequence matter? How is this process regulated? These questions concern all eukaryotes but have proven extremely hard to address because replication timing is a process intricately connected with multiple aspects of nuclear function.
KeywordsDNA replication timing Nuclear architecture Rif1 Nuclear lamina Nuclear organization PP1 Origin firing Telomere replication
I would like to thank past and present members of the Buonomo group for their work that is the base of this review and the invaluable scientific contribution. I am also grateful to Svetlana Makovets and Elizabeth Bayne for critical reading of the manuscript and helpful suggestions. I apologize for the references omitted due to general nature of this review.
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