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Architecture of the Saccharomyces cerevisiae Replisome

  • Lin Bai
  • Zuanning Yuan
  • Jingchuan Sun
  • Roxana Georgescu
  • Michael E. O’DonnellEmail author
  • Huilin LiEmail author
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1042)

Abstract

Eukaryotic replication proteins are highly conserved, and thus study of Saccharomyces cerevisiae replication can inform about this central process in higher eukaryotes including humans. The S. cerevisiae replisome is a large and dynamic assembly comprised of ~50 proteins. The core of the replisome is composed of 31 different proteins including the 11-subunit CMG helicase; RFC clamp loader pentamer; PCNA clamp; the heteroligomeric DNA polymerases ε, δ, and α-primase; and the RPA heterotrimeric single strand binding protein. Many additional protein factors either travel with or transiently associate with these replisome proteins at particular times during replication. In this chapter, we summarize several recent structural studies on the S. cerevisiae replisome and its subassemblies using single particle electron microscopy and X-ray crystallography. These recent structural studies have outlined the overall architecture of a core replisome subassembly and shed new light on the mechanism of eukaryotic replication.

Keywords

Eukaryotic DNA replication Replisome Replicative helicase Cryo-EM Mcm CMG DNA polymerase Structural biology 

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© Springer Nature Singapore Pte Ltd. 2017

Authors and Affiliations

  1. 1.Cryo-EM Structural Biology LaboratoryVan Andel Research InstituteGrand RapidsUSA
  2. 2.Biochemistry and Structural Biology Graduate ProgramStony Brook UniversityStony BrookUSA
  3. 3.Howard Hughes Medical InstituteThe Rockefeller UniversityNew YorkUSA

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