Abstract
Due to the structural similarity to the extracellular matrix, nowadays, hydrogels are widely used for tissue engineering applications. Among the various hydrogels, alginate is considered a very useful biomaterial that has found numerous applications in the biomedical field due to its favorable properties, including biocompatibility and ease of gelation. It has been used to design tissue engineering constructs of various structures, such as porous scaffolds, microspheres, films, and microcapsules for drug and cell delivery and various tissue engineering applications particularly for bone, cartilage, muscle, and vascular tissue engineering. This chapter will provide a comprehensive overview of the applications of alginate-based hydrogels as various forms of constructs and scaffolds for tissue engineering.
Keywords
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Lee KY, Mooney DJ (2001) Hydrogels for tissue engineering. Chem Rev 101:1869–1880. https://doi.org/10.1021/cr000108x
Gasperini L, Mano JF, Reis RL (2014) Natural polymers for the microencapsulation of cells. J R Soc Interface 11:20140817. https://doi.org/10.1098/rsif.2014.0817
Cukierman E, Pankov R, Yamada KM (2002) Cell interactions with three-dimensional matrices. Curr Opin Cell Biol 14:633–640. https://doi.org/10.1016/S0955-0674(02)00364-2
Al-shamkhani A, Duncan R (1995) Radioiodination of alginate via covalently-bound tyrosinamide allows monitoring of its fate in vivo. J Bioact Compat Polym 10:4–13. https://doi.org/10.1177/088391159501000102
Wong TY, Preston LA, Schiller NL (2000) Alginate lyase: review of major sources and enzyme characteristics, structure-function analysis, biological roles, and applications. Annu Rev Microbiol 54:289–340. https://doi.org/10.1146/annurev.micro.54.1.289
Rowley JA, Madlambayan G, Mooney DJ (1999) Alginate hydrogels as synthetic extracellular matrix materials. Biomaterials 20:45–53
Boontheekul T, Kong H-J, Mooney DJ (2005) Controlling alginate gel degradation utilizing partial oxidation and bimodal molecular weight distribution. Biomaterials 26:2455–2465. https://doi.org/10.1016/j.biomaterials.2004.06.044
Smetana K (1993) Cell biology of hydrogels. Biomaterials 14:1046–1050. https://doi.org/10.1016/0142-9612(93)90203-E
Price LS (1997) Morphological control of cell growth and viability. Bioessays 19:941–943. https://doi.org/10.1002/bies.950191102
Kim D, Monaco E, Maki A et al (2010) Morphologic and transcriptomic comparison of adipose- and bone-marrow-derived porcine stem cells cultured in alginate hydrogels. Cell Tissue Res 341:359–370. https://doi.org/10.1007/s00441-010-1015-3
Lee KY, Mooney DJ (2012) Alginate: properties and biomedical applications. Prog Polym Sci 37:106–126. https://doi.org/10.1016/j.progpolymsci.2011.06.003
Kong HJ, Kaigler D, Kim K, Mooney DJ (2004) Controlling rigidity and degradation of alginate hydrogels via molecular weight distribution. Biomacromolecules 5:1720–1727. https://doi.org/10.1021/bm049879r
Balakrishnan B, Jayakrishnan A (2005) Self-cross-linking biopolymers as injectable in situ forming biodegradable scaffolds. Biomaterials 26:3941–3951. https://doi.org/10.1016/j.biomaterials.2004.10.005
Liao H, Zhang H, Chen W (2009) Differential physical, rheological, and biological properties of rapid in situ gelable hydrogels composed of oxidized alginate and gelatin derived from marine or porcine sources. J Mater Sci Mater Med 20:1263–1271. https://doi.org/10.1007/s10856-009-3694-4
Boanini E, Rubini K, Panzavolta S, Bigi A (2010) Chemico-physical characterization of gelatin films modified with oxidized alginate. Acta Biomater 6:383–388. https://doi.org/10.1016/j.actbio.2009.06.015
Bouhadir KH, Lee KY, Alsberg E et al (2001) Degradation of partially oxidized alginate and its potential application for tissue engineering. Biotechnol Prog 17:945–950. https://doi.org/10.1021/bp010070p
Balakrishnan B, Mohanty M, Umashankar PR, Jayakrishnan a (2005) Evaluation of an in situ forming hydrogel wound dressing based on oxidized alginate and gelatin. Biomaterials 26:6335–6342. https://doi.org/10.1016/j.biomaterials.2005.04.012
Sarker B, Papageorgiou DG, Silva R et al (2014) Fabrication of alginate–gelatin crosslinked hydrogel microcapsules and evaluation of the microstructure and physico-chemical properties. J Mater Chem B 2:1470–1482. https://doi.org/10.1039/c3tb21509a
Sarker B, Singh R, Silva R et al (2014) Evaluation of fibroblasts adhesion and proliferation on alginate-gelatin crosslinked hydrogel. PLoS One 9:e107952. https://doi.org/10.1371/journal.pone.0107952
Rangarajan A, Hong SJ, Gifford A, Weinberg RA (2004) Species- and cell type-specific requirements for cellular transformation. Cancer Cell 6:171–183. https://doi.org/10.1016/j.ccr.2004.07.009
Griffith LG, M a S (2006) Capturing complex 3D tissue physiology in vitro. Nat Rev Mol Cell Biol 7:211–224. https://doi.org/10.1038/nrm1858
Liu X, Ma X (2004) Polymeric scaffolds for bone tissue engineering. Ann Biomed Eng 32:477–486
Annabi N, Nichol JW, Zhong X et al (2010) Controlling the porosity and microarchitecture of hydrogels for tissue engineering. Tissue Eng Part B Rev 16:371–383. https://doi.org/10.1089/ten.teb.2009.0639
Zmora S, Glicklis R, Cohen S (2002) Tailoring the pore architecture in 3-D alginate scaffolds by controlling the freezing regime during fabrication. Biomaterials 23:4087–4094
Sarker B, Hum J, Nazhat SN, Boccaccini AR (2015) Combining collagen and bioactive glasses for bone tissue engineering: a review. Adv Healthc Mater 4:176–194. https://doi.org/10.1002/adhm.201400302
Wang Y, Yang C, Chen X, Zhao N (2006) Development and characterization of novel biomimetic composite scaffolds based on bioglass-collagen-hyaluronic acid-phosphatidylserine for tissue engineering applications. Macromol Mater Eng 291:254–262. https://doi.org/10.1002/mame.200500381
Sun J, Tan H (2013) Alginate-based biomaterials for regenerative medicine applications. Materials (Basel) 6:1285–1309. https://doi.org/10.3390/ma6041285
Shapiro L, Cohen S (1997) Novel alginate sponges for cell culture and transplantation. Biomaterials 18:583–590. https://doi.org/10.1016/S0142-9612(96)00181-0
Sapir Y, Kryukov O, Cohen S (2011) Integration of multiple cell-matrix interactions into alginate scaffolds for promoting cardiac tissue regeneration. Biomaterials 32:1838–1847. https://doi.org/10.1016/j.biomaterials.2010.11.008
Florczyk SJ, Kim DJ, Wood DL, Zhang M (2011) Influence of processing parameters on pore structure of 3D porous chitosan-alginate polyelectrolyte complex scaffolds. J Biomed Mater Res – Part A 98(A):614–620. https://doi.org/10.1002/jbm.a.33153
Li Z, Ramay HR, Hauch KD et al (2005) Chitosan-alginate hybrid scaffolds for bone tissue engineering. Biomaterials 26:3919–3928. https://doi.org/10.1016/j.biomaterials.2004.09.062
Petrenko YA, Ivanov RV, Petrenko a Y, Lozinsky VI (2011) Coupling of gelatin to inner surfaces of pore walls in spongy alginate-based scaffolds facilitates the adhesion, growth and differentiation of human bone marrow mesenchymal stromal cells. J Mater Sci Mater Med 22:1529–1540. https://doi.org/10.1007/s10856-011-4323-6
Luo Z, Yang Y, Deng Y et al (2016) Peptide-incorporated 3D porous alginate scaffolds with enhanced osteogenesis for bone tissue engineering. Colloids Surf B Biointerfaces 143:243–251. https://doi.org/10.1016/j.colsurfb.2016.03.047
Yang C, Frei H, Rossi FM, Burt HM (2009) The differential in vitro and in vivo responses of bone marrow stromal cells on novel porous gelatin-alginate scaffolds. J Tissue Eng Regen Med 3:601–614. https://doi.org/10.1002/term.201
Florczyk SJ, Leung M, Li Z et al (2013) Evaluation of three-dimensional porous chitosan-alginate scaffolds in rat calvarial defects for bone regeneration applications. J Biomed Mater Res Part A 101:2974–2983. https://doi.org/10.1002/jbm.a.34593
Lin H-R, Yeh Y-J (2004) Porous alginate/hydroxyapatite composite scaffolds for bone tissue engineering: Preparation, characterization, and in vitro studies. J Biomed Mater Res 71B:52–65. https://doi.org/10.1002/jbm.b.30065
Rajesh R, Ravichandran D (2015) Development of a new carbon nanotube – alginate – hydroxyapatite tricomponent composite scaffold for application in bone tissue engineering. Int J Nanomedicine 10:7–15
Sowjanya JA, Singh J, Mohita T et al (2013) Biocomposite scaffolds containing chitosan/alginate/nano-silica for bone tissue engineering. Colloids Surfs B Biointerfaces 109:294–300. https://doi.org/10.1016/j.colsurfb.2013.04.006
Mishra R, Basu B, Kumar A (2009) Physical and cytocompatibility properties of bioactive glass-polyvinyl alcohol-sodium alginate biocomposite foams prepared via sol-gel processing for trabecular bone regeneration. J Mater Sci Mater Med 20:2493–2500. https://doi.org/10.1007/s10856-009-3814-1
Suárez-González D, Barnhart K, Saito E et al (2010) Controlled nucleation of hydroxyapatite on alginate scaffolds for stem cell-based bone tissue engineering. J Biomed Mater Res Part A 95A:222–234. https://doi.org/10.1002/jbm.a.32833
Sarker B, Li W, Zheng K et al (2016) Designing porous bone tissue engineering scaffolds with enhanced mechanical properties from composite hydrogels composed of modified alginate, gelatin, and bioactive glass. ACS Biomater Sci Eng. acsbiomaterials.6b00470. https://doi.org/10.1021/acsbiomaterials.6b00470
Cai K, Zhang J, Deng L et al (2007) Physical and biological properties of a novel hydrogel composite based on oxidized alginate, gelatin and tricalcium phosphate for bone tissue engineering. Adv Eng Mater 9:1082–1088. https://doi.org/10.1002/adem.200700222
Li Z, Zhang M (2005) Chitosan-alginate as scaffolding material for cartilage tissue engineering. J Biomed Mater Res Part A 75:485–493. https://doi.org/10.1002/jbm.a.30449
Benya P (1982) Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell 30:215–224. https://doi.org/10.1016/0092-8674(82)90027-7
Homicz MR, Chia SH, Schumacher BL et al (2003) Human septal chondrocyte redifferentiation in alginate, polyglycolic acid scaffold, and monolayer culture. Laryngoscope 113:25–32. https://doi.org/10.1097/00005537-200301000-00005
Hsu SH, Shu WW, Hsieh SC et al (2004) Evaluation of chitosan-alginate-hyaluronate complexes modified by an RGD-containing protein as tissue-engineering scaffolds for cartilage regeneration. Artif Organs 28:693–703. https://doi.org/10.1111/j.1525-1594.2004.00046.x
Re’em T, Tsur-Gang O, Cohen S (2010) The effect of immobilized RGD peptide in macroporous alginate scaffolds on TGFβ1-induced chondrogenesis of human mesenchymal stem cells. Biomaterials 31:6746–6755. https://doi.org/10.1016/j.biomaterials.2010.05.025
Wang L, Shansky J, Borselli C et al (2012) Design and fabrication of a biodegradable, covalently crosslinked shape-memory alginate scaffold for cell and growth factor delivery. Tissue Eng Part A 18:2000–2007. https://doi.org/10.1089/ten.tea.2011.0663
Chen F, Tian M, Zhang D et al (2012) Preparation and characterization of oxidized alginate covalently cross-linked galactosylated chitosan scaffold for liver tissue engineering. Mater Sci Eng C 32:310–320. https://doi.org/10.1016/j.msec.2011.10.034
Sapir Y, Cohen S, Friedman G, Polyak B (2012) The promotion of in vitro vessel-like organization of endothelial cells in magnetically responsive alginate scaffolds. Biomaterials 33:4100–4109. https://doi.org/10.1016/j.biomaterials.2012.02.037
Murphy SV, Skardal A, Atala A (2013) Evaluation of hydrogels for bio-printing applications. J Biomed Mater Res A 101:272–284. https://doi.org/10.1002/jbm.a.34326
Luo Y, Lode A, Wu C et al (2015) Alginate/nanohydroxyapatite scaffolds with designed core/shell structures fabricated by 3D plotting and in situ mineralization for bone tissue engineering. ACS Appl Mater Interfaces 7:6541–6549. https://doi.org/10.1021/am508469h
Luo Y, Wu C, Lode A, Gelinsky M (2013) Hierarchical mesoporous bioactive glass/alginate composite scaffolds fabricated by three-dimensional plotting for bone tissue engineering. Biofabrication 5:15005. https://doi.org/10.1088/1758-5082/5/1/015005
Wang X, Tolba E, Der HCS et al (2014) Effect of bioglass on growth and biomineralization of saos-2 cells in hydrogel after 3d cell bioprinting. PLoS One 9:1–7. https://doi.org/10.1371/journal.pone.0112497
Lee H, Ahn S-H, Kim GH (2012) Three-dimensional collagen/alginate hybrid scaffolds functionalized with a drug delivery system (DDS) for bone tissue regeneration. Chem Mater 24:881–891. https://doi.org/10.1021/cm200733s
Zehnder T, Sarker B, Boccaccini AR, Detsch R (2015) Evaluation of an alginate–gelatine crosslinked hydrogel for bioplotting. Biofabrication 7:1–12. https://doi.org/10.1088/1758-5090/7/2/025001
Grigore A, Sarker B, Fabry B et al (2014) Behavior of encapsulated MG-63 cells in RGD and gelatine-modified alginate hydrogels. Tissue Eng Part A 20:2140–2150. https://doi.org/10.1089/ten.tea.2013.0416
Detsch R, Sarker B, Zehnder T et al (2015) Advanced alginate-based hydrogels. Mater Today 18:590–591. https://doi.org/10.1016/j.mattod.2015.10.013
Sarker B, Rompf J, Silva R et al (2015) Alginate-based hydrogels with improved adhesive properties for cell encapsulation. Int J Biol Macromol 78:72–78. https://doi.org/10.1016/j.ijbiomac.2015.03.061
Leite ÁJ, Sarker B, Zehnder T et al (2016) Bioplotting of a bioactive alginate dialdehyde-gelatin composite hydrogel containing bioactive glass nanoparticles. Biofabrication 8:35005. https://doi.org/10.1088/1758-5090/8/3/035005
Marijnissen WJC, van Osch GJV, Aigner J et al (2002) Alginate as a chondrocyte-delivery substance in combination with a non-woven scaffold for cartilage tissue engineering. Biomaterials 23:1511–1517. https://doi.org/10.1016/S0142-9612(01)00281-2
Kundu J, Shim J-H, Jang J et al (2015) An additive manufacturing-based PCL-alginate-chondrocyte bioprinted scaffold for cartilage tissue engineering. J Tissue Eng Regen Med 9:1286–1297. https://doi.org/10.1002/term.1682
Narayanan LK, Huebner P, Fisher MB et al (2016) 3D-bioprinting of polylactic acid (PLA) nanofiber–alginate hydrogel bioink containing human adipose-derived stem cells. ACS Biomater Sci Eng 2:1732–1742. https://doi.org/10.1021/acsbiomaterials.6b00196
Markstedt K, Mantas A, Tournier I et al (2015) 3D bioprinting human chondrocytes with nanocellulose-alginate bioink for cartilage tissue engineering applications. Biomacromolecules 16:1489–1496. https://doi.org/10.1021/acs.biomac.5b00188
Wang CC, Yang KC, Lin KH et al (2011) A highly organized three-dimensional alginate scaffold for cartilage tissue engineering prepared by microfluidic technology. Biomaterials 32:7118–7126. https://doi.org/10.1016/j.biomaterials.2011.06.018
Park J, Lee SJ, Chung S et al (2017) Cell-laden 3D bioprinting hydrogel matrix depending on different compositions for soft tissue engineering: characterization and evaluation. Mater Sci Eng C 71:678–684. https://doi.org/10.1016/j.msec.2016.10.069
Gao Q, He Y, J zhong F et al (2015) Coaxial nozzle-assisted 3D bioprinting with built-in microchannels for nutrients delivery. Biomaterials 61:203–215. https://doi.org/10.1016/j.biomaterials.2015.05.031
Man Y, Wang P, Guo Y et al (2012) Angiogenic and osteogenic potential of platelet-rich plasma and adipose-derived stem cell laden alginate microspheres. Biomaterials 33:8802–8811. https://doi.org/10.1016/j.biomaterials.2012.08.054
Ausländer S, Wieland M, Fussenegger M (2012) Smart medication through combination of synthetic biology and cell microencapsulation. Metab Eng 14:252–260. https://doi.org/10.1016/j.ymben.2011.06.003
Li H-B, Jiang H, Wang C-Y et al (2006) Comparison of two types of alginate microcapsules on stability and biocompatibility in vitro and in vivo. Biomed Mater 1:42–47. https://doi.org/10.1088/1748-6041/1/1/007
Schacht K, Jüngst T, Schweinlin M et al (2015) Biofabrication of cell-loaded 3D spider silk constructs. Angew Chemie Int Ed 54:2816–2820. https://doi.org/10.1002/anie.201409846
Dhote V, Skaalure S, Akalp U et al (2013) On the role of hydrogel structure and degradation in controlling the transport of cell-secreted matrix molecules for engineered cartilage. J Mech Behav Biomed Mater 19:61–74. https://doi.org/10.1016/j.jmbbm.2012.10.016
Lim F, Sun A (1980) Microencapsulated islets as bioartificial endocrine pancreas. Science 210:908–910. https://doi.org/10.1126/science.6776628
Abbah SA, WW L, Chan D et al (2006) In vitro evaluation of alginate encapsulated adipose-tissue stromal cells for use as injectable bone graft substitute. Biochem Biophys Res Commun 347:185–191. https://doi.org/10.1016/j.bbrc.2006.06.072
Moshaverinia A, Ansari S, Chen C et al (2013) Co-encapsulation of anti-BMP2 monoclonal antibody and mesenchymal stem cells in alginate microspheres for bone tissue engineering. Biomaterials 34:6572–6579. https://doi.org/10.1016/j.biomaterials.2013.05.048
Freire MO, You H-K, Kook J-K et al (2011) Antibody-mediated osseous regeneration: a novel strategy for bioengineering bone by immobilized anti–bone morphogenetic protein-2 antibodies. Tissue Eng Part A 17:2911–2918. https://doi.org/10.1089/ten.tea.2010.0584
Hwang Y-S, Cho J, Tay F et al (2009) The use of murine embryonic stem cells, alginate encapsulation, and rotary microgravity bioreactor in bone tissue engineering. Biomaterials 30:499–507. https://doi.org/10.1016/j.biomaterials.2008.07.028
Wang H, Lee J-K, Moursi A, Lannutti JJ (2003) Ca/P ratio effects on the degradation of hydroxyapatite in vitro. J Biomed Mater Res A 67:599–608. https://doi.org/10.1002/jbm.a.10538
Arpornmaeklong P, Kochel M, Depprich R et al (2004) Influence of platelet-rich plasma (PRP) on osteogenic differentiation of rat bone marrow stromal cells. An in vitro study. Int J Oral Maxillofac Surg 33:60–70. https://doi.org/10.1054/ijom.2003.0492
Kanno T, Takahashi T, Tsujisawa T et al (2005) Platelet-rich plasma enhances human osteoblast-like cell proliferation and differentiation. J Oral Maxillofac Surg 63:362–369. https://doi.org/10.1016/j.joms.2004.07.016
Rottensteiner U, Sarker B, Heusinger D et al (2014) In vitro and in vivo biocompatibility of alginate dialdehyde/gelatin hydrogels with and without nanoscaled bioactive glass for bone tissue engineering applications. Materials (Basel) 7:1957–1974. https://doi.org/10.3390/ma7031957
Song K, Yang Y, Li S et al (2014) In vitro culture and oxygen consumption of NSCs in size-controlled neurospheres of Ca-alginate/gelatin microbead. Mater Sci Eng C Mater Biol Appl 40:197–203. https://doi.org/10.1016/j.msec.2014.03.028
Silva R, Singh R, Sarker B et al (2014) Hybrid hydrogels based on keratin and alginate for tissue engineering. J Mater Chem B 2:5441–5451. https://doi.org/10.1039/c4tb00776j
Silva R, Singh R, Sarker B et al (2016) Soft-matrices based on silk fibroin and alginate for tissue engineering. Int J Biol Macromol 2:5441–5451. https://doi.org/10.1016/j.ijbiomac.2016.04.045
Zhou H, HHK X (2011) The fast release of stem cells from alginate-fibrin microbeads in injectable scaffolds for bone tissue engineering. Biomaterials 32:7503–7513. https://doi.org/10.1016/j.biomaterials.2011.06.045
Zeng Q, Han Y, Li H, Chang J (2014) Bioglass/alginate composite hydrogel beads as cell carriers for bone regeneration. J Biomed Mater Res Part B Appl Biomater 102:42–51. https://doi.org/10.1002/jbm.b.32978
Gorustovich A a, Roether JA, Boccaccini AR (2010) Effect of bioactive glasses on angiogenesis: a review of in vitro and in vivo evidences. Tissue Eng Part B Rev 16:199–207. https://doi.org/10.1089/ten.TEB.2009.0416
Hoppe A, Güldal NS, Boccaccini AR (2011) A review of the biological response to ionic dissolution products from bioactive glasses and glass-ceramics. Biomaterials 32:2757–2774
Moshaverinia A, Xu X, Chen C et al (2013) Dental mesenchymal stem cells encapsulated in an alginate hydrogel co-delivery microencapsulation system for cartilage regeneration. Acta Biomater 9:9343–9350. https://doi.org/10.1016/j.actbio.2013.07.023
Guo J, Jourdian GW, Maccallum DK (1989) Culture and growth characteristics of chondrocytes encapsulated in alginate beads. Connect Tissue Res 19:277–297. https://doi.org/10.3109/03008208909043901
Ma H-L, Hung S-C, Lin S-Y et al (2003) Chondrogenesis of human mesenchymal stem cells encapsulated in alginate beads. J Biomed Mater Res 64A:273–281. https://doi.org/10.1002/jbm.a.10370
Endres M, Wenda N, Woehlecke H et al (2010) Microencapsulation and chondrogenic differentiation of human mesenchymal progenitor cells from subchondral bone marrow in Ca-alginate for cell injection. Acta Biomater 6:436–444. https://doi.org/10.1016/j.actbio.2009.07.022
Chang J-C, Hsu S-H, Chen DC (2009) The promotion of chondrogenesis in adipose-derived adult stem cells by an RGD-chimeric protein in 3D alginate culture. Biomaterials 30:6265–6275. https://doi.org/10.1016/j.biomaterials.2009.07.064
Focaroli S, Teti G, Salvatore V et al (2016) Calcium/cobalt alginate beads as functional scaffolds for cartilage tissue engineering. Stem Cells Int 2016:1–12. https://doi.org/10.1155/2016/2030478
Gaetani P, Torre ML, Klinger M et al (2008) Adipose-derived stem cell therapy for intervertebral disc regeneration: an in vitro reconstructed tissue in alginate capsules. Tissue Eng Part A 14:1415–1423. https://doi.org/10.1089/ten.tea.2007.0330
Ansari S, Chen C, Xu X et al (2016) Muscle tissue engineering using gingival mesenchymal stem cells encapsulated in alginate hydrogels containing multiple growth factors. Ann Biomed Eng 44:1908–1920. https://doi.org/10.1007/s10439-016-1594-6
Kreeger PK, Deck JW, Woodruff TK, Shea LD (2006) The in vitro regulation of ovarian follicle development using alginate-extracellular matrix gels. Biomaterials 27:714–723. https://doi.org/10.1016/j.biomaterials.2005.06.016
Manju S, Muraleedharan CV, Rajeev A et al (2011) Evaluation of alginate dialdehyde cross-linked gelatin hydrogel as a biodegradable sealant for polyester vascular graft. J Biomed Mater Res B Appl Biomater 98:139–149. https://doi.org/10.1002/jbm.b.31843
Yu J, KT D, Fang Q et al (2010) The use of human mesenchymal stem cells encapsulated in RGD modified alginate microspheres in the repair of myocardial infarction in the rat. Biomaterials 31:7012–7020. https://doi.org/10.1016/j.biomaterials.2010.05.078
Engler AJ, Sen S, Sweeney HL, Discher DE (2006) Matrix elasticity directs stem cell lineage specification. Cell 126:677–689. https://doi.org/10.1016/j.cell.2006.06.044
Moshaverinia A, Xu X, Chen C et al (2014) Application of stem cells derived from the periodontal ligament orgingival tissue sources for tendon tissue regeneration. Biomaterials 35:2642–2650. https://doi.org/10.1016/j.biomaterials.2013.12.053
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Sarker, B., Boccaccini, A.R. (2018). Alginate Utilization in Tissue Engineering and Cell Therapy. In: Rehm, B., Moradali, M. (eds) Alginates and Their Biomedical Applications. Springer Series in Biomaterials Science and Engineering, vol 11. Springer, Singapore. https://doi.org/10.1007/978-981-10-6910-9_5
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