Abstract
Metabolomics is an important technique that detects change in the quantitative profile of the organism to observe its response towards diverse genetic alterations and environmental conditions. In the present study, metabolite profiling of Heterodermia diademata, a common foliose lichen in Garhwal Himalayas, has been carried out in different environmental niches within an altitudinal gradients of 700–1850 m a.s.l. especially for its metabolites to decode any environmental significance. Metabolites, namely, fatty acid (FA), amino acid (AA) and sugar, were quantified using LC-MS/MS technique. Fatty acid content showed maximum variation with abundance of unsaturated fatty acid, while amino acid profile is dominated by glutamic acid, proline and serine. Sugar content has maximum variation of lactose. Most of the common primary metabolites have physiological roles as osmoregulators and thermotolerants as well as deterrents quite evident by having high erucic acid content. In the changing temperature gradient, fatty acid profile appears to be a valuable biomarker as it attributes towards thermotolerance of the species. Depending on the environmental condition, H. diademata species displays variation in FA profile, which seems to primarily relate to adaptation. The ability to readily alter the metabolite profile especially fatty acid concentrations appears to be an important contributing factor determining the ubiquity of the species at different altitudes of Garhwal Himalayas.
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Acknowledgements
Director, CSIR-National Botanical Research Institute, Lucknow is thankfully acknowledged for facilities and encouragements. VS is grateful to the Department of Science and Technology (DST-SERB), New Delhi, for award of Young Scientist fellowship (SR/FTP/ES-39/2013).
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Shukla, V., Asati, A., Patel, D.K., Semwal, M., Kumar, N., Upreti, D.K. (2017). Metabolic Profiling and Its Plausible Environmental Significance in a Common Himalayan Lichen. In: Shukla, V., Kumar, S., Kumar, N. (eds) Plant Adaptation Strategies in Changing Environment. Springer, Singapore. https://doi.org/10.1007/978-981-10-6744-0_9
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