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Preparation and Physical Properties of Asymmetric Model Membrane Vesicles

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The Biophysics of Cell Membranes

Part of the book series: Springer Series in Biophysics ((BIOPHYSICS,volume 19))

Abstract

Model biomembrane vesicles composed of lipids have been widely used to investigate the principles of membrane assembly and organization. A limitation of these vesicles has been that they do not mimic the transbilayer lipid asymmetry seen in many natural membranes, most notably the asymmetry in the plasma membrane of eukaryotic cells. Recently, a number of approaches have been developed to prepare asymmetric membranes and study their properties. This review describes methods to prepare asymmetric model membranes, and the physical properties of asymmetric lipid vesicles. Emphasis is placed on the vesicles prepared by cyclodextrin-catalyzed exchange, which has proven to be a versatile and powerful tool, including for studies manipulating lipid asymmetry in living cells.

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Acknowledgement

Support from NIH grant GM 112638 and GM 122493 is gratefully acknowledged.

Author information

Authors and Affiliations

  1. Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, 11794-5215, USA

    Johnna R. St. Clair, Qing Wang, Guangtao Li & Erwin London

Authors
  1. Johnna R. St. Clair
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  2. Qing Wang
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  3. Guangtao Li
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  4. Erwin London
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Corresponding author

Correspondence to Erwin London .

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Editors and Affiliations

  1. Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada

    Richard M. Epand

  2. Sciences Faculty, Université Libre de Bruxelles, Bruxelles, Belgium

    Jean-Marie Ruysschaert

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© 2017 Springer Nature Singapore Pte Ltd.

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St. Clair, J.R., Wang, Q., Li, G., London, E. (2017). Preparation and Physical Properties of Asymmetric Model Membrane Vesicles. In: Epand, R., Ruysschaert, JM. (eds) The Biophysics of Cell Membranes. Springer Series in Biophysics, vol 19. Springer, Singapore. https://doi.org/10.1007/978-981-10-6244-5_1

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