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Basic Techniques in Fluorescence and Electron Microscopy

  • Haruko KuroiwaEmail author
Chapter

Abstract

The primitive red alga Cyanidioschyzon merolae is a single cell with a very small size (1.5–2 μm) and has no thick cell wall; therefore, preparation of samples for fluorescence and electron microscopy requires different procedures compared to those for green algae or higher plants. Here, fluorescence microscopy methods, such as cell staining with fluorescent dye and the setup of the immunofluorescence microscope, are described. For electron microscopy, methods for transmission electron microscopy, immunoelectron microscopy, and negative staining methods are described. In addition, scanning electron microscopy methods for visualizing cells or organelles are presented.

Keywords

Fluorescence microscopy DAPI Electron microscopy DiOC6 Negative staining Immunoelectron microscopy Scanning microscopy 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2017

Authors and Affiliations

  1. 1.Department of Chemical and Biological Science, Faculty of ScienceJapan Women’s UniversityTokyoJapan

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