The 100%-Complete Nuclear and Organellar Genome Sequences of the Ultrasmall Red Algal Species Cyanidioschyzon merolae 10D
We were the first group to successfully sequence the 100%-complete entire eukaryotic genome in 2007, mainly using automated Sanger sequencing of the unicellular, ultrasmall red algal species Cyanidioschyzon merolae 10D. This world record was principally based on the ultrasmall size of the C. merolae genome (ca. 16 megabase pairs) as well as on three excellent previous studies: the 100%-complete mitochondrial genome in 1998, the 100%-complete plastid genome in 2003, and the first algal cell nuclear genome in 2004. The 100%-complete nuclear sequences demonstrated that this ultrasmall red alga contains unusually simple sets of genes and genetic sequences. For example, because introns are lacking in almost all of the protein-coding nuclear genes of C. merolae, the 100%-complete sequence can be used to directly deduce the sequences of all C. merolae proteins, which will be extremely valuable in further proteomics research. Thus, this small red alga represents an ideal model organism for studying the fundamental relationships among the plastid, mitochondrial, and nuclear genomes. The 100%-complete nuclear genome sequence has greatly improved the precision and value of biological analyses of C. merolae.
KeywordsAlgal genome Cyanidioschyzon merolae Eukaryotic cell Mitochondrial genome Nuclear genome 100%-complete genome Plastid genome Red alga
HN was supported by a Grant-in-Aid for Scientific Research (grant number 16H02518) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT)/Japan Society for the Promotion of Science (JSPS) KAKENHI.
- Barbier G, Oesterhelt C et al (2005) Comparative genomics of two closely related unicellular thermo-acidophilic red algae, Galdieria sulphuraria and Cyanidioschyzon merolae, reveals the molecular basis of the metabolic flexibility of Galdieria sulphuraria and significant differences in carbohydrate metabolism of both algae. Plant Physiol, 137:460–474. https://doi.org/10.1104/pp.104.051169
- Kanesaki Y, Imamura S et al (2012) External light conditions and internal cell cycle phases coordinate accumulation of chloroplast and mitochondrial transcripts in the red alga Cyanidioschyzon merolae. DNA Res 19:289–303. https://doi.org/10.1093/dnares/dss013 CrossRefPubMedPubMedCentralGoogle Scholar
- Martin W, Rujan T et al (2002) Evolutionary analysis of Arabidopsis, cyanobacterial, and chloroplast genomes reveals plastid phylogeny and thousands of cyanobacterial genes in the nucleus. Proc Natl Acad Sci U S A 99:12246–11251. https://doi.org/10.1073/pnas.182432999 CrossRefPubMedPubMedCentralGoogle Scholar
- Taki K, Sone T, Kobayashi Y, Watanabe S, Imamura S, Tanaka K (2015) Construction of a URA5.3 deletion strain of the unicellular red alga Cyanidioschyzon merolae: a background less host strain for transformation experiments. J Gen Appl Microbiol 61:211–214. https://doi.org/10.2323/jgam.61.211