Cell Cycle and Organelle Division Cycle in Cyanidioschyzon merolae

  • Takayuki FujiwaraEmail author


A cell replicates by duplicating cellular contents and then dividing into two daughter cells. This is known as the cell cycle, which is characterized by the dynamics of the cell nucleus. However, eukaryotic cells contain organelles other than the nucleus, such as mitochondria, chloroplasts, peroxisomes, endoplasmic reticulum (ER), Golgi bodies, and vacuoles. To understand reproduction of cells, the division and inheritance of organelles as well as the mitotic cycle should be investigated. However, model animal cells and plant cells contain multiples of these organelles which exhibit complex shapes. Therefore, it has been difficult to elucidate their division and inheritance. In contrast, the red alga Cyanidioschyzon merolae possesses a minimum set of organelles. Because of its simple cellular architecture, the dynamics and mechanisms underlying its cell division and inheritance have been investigated. In addition, cell cycle stages of C. merolae have been determined using an S phase-specific marker proliferating cell nuclear antigen (PCNA) and an M phase-specific marker H3S10ph. In this chapter, I introduce the timing of division and inheritance of organelles during the cell cycle in C. merolae and application of the synchronous culture to transcriptome analysis for identifying novel genes related to the division and inheritance of organelles.


PCNA H3S10ph Cell cycle Organelle division Organelle inheritance Transcriptome 



Our study was partly supported was by JSPS KAKENHI (no. JP15K18588 to T.F.; no. 25251039 to S.M.) and by the Core Research for Evolutional Science and Technology Program of the Japan Science and Technology Agency (S.M.).


  1. Alberts B, Johnson A et al (2007) Molecular biology of the cell. Garland science, 5th edn. Taylor & Francis Group, New YorkGoogle Scholar
  2. Celis JE, Celis A (1985) Cell cycle-dependent variations in the distribution of the nuclear protein cyclin proliferating cell nuclear antigen in cultured cells: subdivision of S phase. Proc Natl Acad Sci U S A 82:3262–3266CrossRefPubMedPubMedCentralGoogle Scholar
  3. Chan DC (2012) Fusion and fission: interlinked processes critical for mitochondrial health. Annu Rev Genet 46:265–287CrossRefPubMedGoogle Scholar
  4. Fujiwara T, Kuroiwa H et al (2010) The coiled-coil protein VIG1 is essential for tethering vacuoles to mitochondria during vacuole inheritance of Cyanidioschyzon merolae. Plant Cell 22:772–781Google Scholar
  5. Fujiwara T, Misumi O et al (2009) Periodic gene expression patterns during the highly synchronized cell nucleus and organelle division cycles in the unicellular red alga Cyanidioschyzon merolae. DNA Res 16:59–72CrossRefPubMedPubMedCentralGoogle Scholar
  6. Fujiwara T, Tanaka K et al (2013) Spatiotemporal dynamics of condensins I and II: evolutionary insights from the primitive red alga Cyanidioschyzon merolae. Mol Biol Cell 24:2515–2527CrossRefPubMedPubMedCentralGoogle Scholar
  7. Hendzel MJ, Wei Y et al (1997) Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation. Chromosoma 106:348–360CrossRefPubMedGoogle Scholar
  8. Imoto Y, Fujiwara T et al (2010) Division of cell nuclei, mitochondria, plastids, and microbodies mediated by mitotic spindle poles in the primitive red alga Cyanidioschyzon merolae. Protoplasma 241:63–74CrossRefPubMedGoogle Scholar
  9. Imoto Y, Yoshida Y et al (2011) The cell cycle, including the mitotic cycle and organelle division cycles, as revealed by cytological observations. J Electron Microsc 60:S117–S136CrossRefGoogle Scholar
  10. Imoto Y, Kuroiwa H et al (2013) Single-membrane-bounded peroxisome division revealed by isolation of dynamin-based machinery. Proc Natl Acad Sci U S A 110:9583–9588CrossRefPubMedPubMedCentralGoogle Scholar
  11. Kuroiwa T, Kuroiwa H et al (1998) The division apparatus of plastids and mitochondria. Int Rev Cytol 181:1–41CrossRefPubMedGoogle Scholar
  12. Maruyama S, Kuroiwa H et al (2007) Centromere dynamics in the primitive red alga Cyanidioschyzon merolae. Plant J 49:1122–1129CrossRefPubMedGoogle Scholar
  13. Misumi O, Matsuzaki M et al (2005) Cyanidioschyzon merolae genome. A tool for facilitating comparable studies on organelle biogenesis in photosynthetic eukaryotes. Plant Physiol 137:567–585CrossRefPubMedPubMedCentralGoogle Scholar
  14. Miyagishima SY, Itoh R et al (1999) Microbody proliferation and segregation cycle in the single-microbody alga Cyanidioschyzon merolae. Planta 208:326–336CrossRefGoogle Scholar
  15. Miyagishima SY, Nishida K et al (2003) A plant-specific dynamin-related protein forms a ring at the chloroplast division site. Plant Cell 15:655–665CrossRefPubMedPubMedCentralGoogle Scholar
  16. Miyagishima SY, Fujiwara T et al (2014) Translation-independent circadian control of the cell cycle in a unicellular photosynthetic eukaryote. Nat Commun 5:3807Google Scholar
  17. Nishida K, Takahara M et al (2003) Dynamic recruitment of dynamin for final mitochondrial severance in a primitive red alga. Proc Natl Acad Sci U S A 100:2146–2151CrossRefPubMedPubMedCentralGoogle Scholar
  18. Nishida K, Yagisawa F et al (2005) Cell cycle-regulated, microtubule-independent organelle division in Cyanidioschyzon merolae. Mol Biol Cell 16:2493–2502CrossRefPubMedPubMedCentralGoogle Scholar
  19. Nishida K, Yagisawa F et al (2007) WD40 protein Mda1 is purified with Dnm1 and forms a dividing ring for mitochondria before Dnm1 in Cyanidioschyzon merolae. Proc Natl Acad Sci U S A 104:4736–4741CrossRefPubMedPubMedCentralGoogle Scholar
  20. Suzuki K, Ehara T et al (1994) Behavior of mitochondria, chloroplasts and their nuclei during the mitotic cycle in the ultramicroalga Cyanidioschyzon merolae. Eur J Cell Biol 63:280–288Google Scholar
  21. Watson JD, Hopkins NH et al (1987) Molecular biology of the gene. The Benjamin/Cummings Publishing Company, Inc., Menlo ParkGoogle Scholar
  22. Yagisawa F, Nishida K et al (2007) Identification and mitotic partitioning strategies of vacuoles in the unicellular red alga Cyanidioschyzon merolae. Planta 226:1017–1022CrossRefPubMedGoogle Scholar
  23. Yagisawa F, Fujiwara T et al (2012) Mitotic inheritance of endoplasmic reticulum in the primitive red alga Cyanidioschyzon merolae. Protoplasma 249:1129–1135CrossRefPubMedGoogle Scholar
  24. Yagisawa F, Fujiwara T et al (2013) Golgi inheritance in the primitive red alga, Cyanidioschyzon merolae. Protoplasma 250:943–948CrossRefPubMedGoogle Scholar
  25. Yoshida Y, Kuroiwa H et al (2009) The bacterial ZapA-like protein ZED is required for mitochondrial division. Curr Biol 19:1491–1497CrossRefPubMedGoogle Scholar
  26. Yoshida Y, Kuroiwa H et al (2010) Chloroplasts divide by contraction of a bundle of nanofilaments consisting of polyglucan. Science 329:949–953CrossRefPubMedGoogle Scholar

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© Springer Nature Singapore Pte Ltd. 2017

Authors and Affiliations

  1. 1.Department of Cell GeneticsNational Institute of GeneticsShi-zuokaJapan
  2. 2.Department of GeneticsGraduate University for Advanced Studies (SOKENDAI), 1111 YataMishimaJapan
  3. 3.Japan Science and Technology Agency, CRESTSaitamaJapan

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