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Technical Basis for Nuclear Magnetic Resonance Approach for Glycoproteins

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Experimental Approaches of NMR Spectroscopy

Abstract

Glycophobia in structural biology is strongly associated with the unpredictable, heterogeneous nature of protein glycosylation and the complex, flexible structures of the glycoprotein glycans. Moreover, glycoproteins cannot be produced by conventional bacterial expression systems. Nuclear magnetic resonance (NMR) spectroscopy assisted by other analytical and preparative techniques can now successfully address these issues. Recombinant glycoproteins can be expressed with stable isotope labeling using a variety of eukaryotic production vehicles. Glycoforms of glycoproteins can be remodeled by genetic engineering of the production vehicles as well as in vitro enzymatic reactions. Stable-isotope-assisted NMR techniques have provided detailed information regarding conformational dynamics and interactions of the carbohydrate chains in solution, giving insights into the functional mechanisms of glycoprotein glycans.

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Acknowledgements

We thank Mr. Masaki Kato (Structural Glycobiology Team, RIKEN) for statistic analysis of PDB data and Drs. Takumi Yamaguchi (School of Material Science, Japan Advanced Institute of Science and Technology) and Maho Yagi-Utsumi (Institute for Molecular Science, National Institutes of Natural Sciences) for useful discussion. This work was partly supported by the Nanotechnology Platform Program (Molecule and Material Synthesis) of MEXT, MEXT/JSPS Grants in Aid for Scientific Research (JP25102008, JP15K07935 and JP17H05893), the Japan Agency for Medical Research and Development, and the Cooperative Research Program of Institute for Protein Research, Osaka University, NMRCR-16-05.

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Authors and Affiliations

  1. Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-Ku, Nagoya, 467-8603, Japan

    Koichi Kato & Hirokazu Yagi

  2. Okazaki Institute for Integrative Bioscience, Institute for Molecular Science, National Institutes of Natural Sciences, 5-1 Higashiyama Myodaiji, Okazaki, 444-8787, Japan

    Koichi Kato & Saeko Yanaka

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  1. Koichi Kato
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  2. Saeko Yanaka
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  3. Hirokazu Yagi
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Correspondence to Koichi Kato .

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    The Nuclear Magnetic Resonance Society of Japan

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Kato, K., Yanaka, S., Yagi, H. (2018). Technical Basis for Nuclear Magnetic Resonance Approach for Glycoproteins. In: The Nuclear Magnetic Resonance Society of Japan (eds) Experimental Approaches of NMR Spectroscopy. Springer, Singapore. https://doi.org/10.1007/978-981-10-5966-7_15

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