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Impact of Light-Emitting Diodes (LEDs) on Propagation of Orchids in Tissue Culture

Chapter

Abstract

LED technology is increasingly used in organ and tissue cultures of ornamental plants due to its ability to adjust the spectral light composition to the physiological and morphological requirements of plants. This chapter presents an overview of studies that have been conducted to investigate the effects of light-emitting diodes (LEDs) on the propagation of different orchids under in vitro condition. Most of the available reports are related to the use of mixed red and blue LEDs. In general, monochromatic red light enhances vegetative growth, whereas blue light usually increases chlorophyll content in the regenerated plantlets. There are some particular species in which beneficial effects of green LED light have been ascertained on the induction of protocorm-like bodies (PLBs). The application of far-red in combination with red light can be effective in the improvement of the quality of micropropagated plantlets. However, the results vary depending on tested spectral composition of light, species, or even cultivar as well as applied environmental conditions. This review also addresses future challenges in the adoption of LED technology for improving the growth and development of plantlets cultivated under ex vitro conditions.

Keywords

LED light Spectral quality Protocorm Morphogenetic response Plantlet acclimation 

References

  1. Ali M, Hahn EJ, Peak HY (2005) Effects of light intensities on antioxidant enzymes and malondialdehyde content during short-term acclimatization on micropropagated Phalenopsis plantlet. Environ Exp Bot 54:109–120 CrossRefGoogle Scholar
  2. Arditti J (2008) Micropropagation of orchids. Blackwell Publ, USA. ISBN-13:978-1-4058–6088-9Google Scholar
  3. Bae KH, Oh KH, Kim SY (2014) Sodium hypochlorite treatment and light-emitting diode (LED) irradiation effect on in vitro germination of Oreorchis patens (Lindl.) Lindl. J Plant Biotech 41:44–49CrossRefGoogle Scholar
  4. Ballantyne M, Pickering CM (2015) The impacts of trail infrastructure on vegetation and soils: current literature and future directions. J Environ Man 164:53–64CrossRefGoogle Scholar
  5. Baque AM, Shin YK, Elshmari T, Lee EJ, Paek KY (2011) Effect of light quality, sucrose and coconut water concentration on the microporpagation of Calanthe hybrids (‘Bukduseong’ × ‘Hyesung’ and ‘Chunkwang’ × ‘Hyesung’). Aust J Crop Sci 5(10):1247–1254Google Scholar
  6. Bateman RM (2011) Two steps forward, one step back: deciphering British and irish marsch-orchids. Watsonia 14:347–376Google Scholar
  7. Batschauer A (1999) Light perception in higher plants. Cell Mol Life Sci 55(2):153–166Google Scholar
  8. Battacharyya P, Kumaria S, Job N, Tandon P (2015) Phyto-molecular profiling and assessment of antioxidant activity within micropropagated plants of Dendrobium thyrsiflorum: a threatened, medicinal orchid. Plant Cell Tissue Organ Cult 122:535–550CrossRefGoogle Scholar
  9. Begum AA, Tamaki M, Kako S (1994) Formation of protocorm-like bodies (PLBs) and shoot development through in vitro culture of outer tissue of Cymbidium PLB. J. Jpn Hortic Sci 63(3):663–637Google Scholar
  10. Bhadra SK, Hossain MM (2003) In vitro germination and micropropagation of Geodorum densiflorum (Lam.) Schltr., an endangered orchid species. Plant Tissue Cult 13(2):165–171Google Scholar
  11. Bhattacharyya P, Van Staden J (2016) Ansellia africana (Leopard orchid): a medicinal orchid species with untapped reserves of important biomolecules—a mini review. S Afr J Bot 106:181–185CrossRefGoogle Scholar
  12. Bhattacharyya P, Kumaria S, Tandon P (2016) High frequency regeneration protocol for Dendrobium nobile: a model tissue culture approach for propagation of medicinally important orchid species. S Afr J Bot 104:232–243CrossRefGoogle Scholar
  13. Chen Y, Goodale UM, Fan XL, Gao JY (2015) Asymbiotic seed germination and in vitro seedling development of Paphiopedilum spicerianum: an orchid with an extremely small population in China. Global Ecol Conserv 3:367–378CrossRefGoogle Scholar
  14. Chia TF, Hew CS, Loh CS, Lee YK (1998) Carbon/nitrogen ratio and greening and protocorm formation in orchid callus tissue. HortScience 13:599–601Google Scholar
  15. Chugh S, Satyakam G, Rao IU (2009) Micropropagation of orchids: a review on the potential of different explants. Sci Hortic 122:507–520CrossRefGoogle Scholar
  16. Chung JP, Huang CY, Dai TE (2010) Spectral effects on embryogenesis and plantlet growth of Oncidium ‘Gower Ramsey’. Sci Hortic 124:511–516CrossRefGoogle Scholar
  17. Cybularz-Urban T, Hanus-Fajerska E (2008) The morphogenetic capability and the viability of regenerants in micropropagated orchid hybrids infected with viral pathogens. Folia Hortic 20(2):93–102CrossRefGoogle Scholar
  18. Cybularz-Urban T, Hanus-Fajerska E, Swiderski A (2007a) Effect of wavelength on in vitro organogenesis of Cattleya hybrid. Act Biol Cracoc, Series Bot 49(1):113–118Google Scholar
  19. Cybularz-Urban T, Hanus-Fajerska E, Swiderski A (2007b) Preliminary morphological, anatomical and biochemical characteristic of micropropagated Cattleya under UV-A and white light illumination. Zesz Probl Post Nauk Roln 523:59–67Google Scholar
  20. Cybularz-Urban T, Hanus-Fajerska E, Bach A (2015) Callus induction and organogenesis in vitro of Cattleya protocorm-like bodies (PLBs) under different light conditions. Act Sci Pol, Seria Hort Cult Hortic 14(6):19–28Google Scholar
  21. da Silva JAT (2014) The response of protocorm-like bodies of nine hybrid Cymbidium cultivars to light-emitting diodes. Environ Exp Biol 12:155–159Google Scholar
  22. da Silva JAT, Zeng S, Cardoso J, Dobránszki J, Kerbauy GB (2014) In vitro flowering of Dendrobium. Plant Cell Tissue Organ Cult 119:447–456CrossRefGoogle Scholar
  23. de la Rosa-Manzano E, Andarade JL, Zotz G, Reyes-García C (2014) Epiphytic orchids in tropical dry forests in Yucatan, Mexico–species occurrence, abundance and correlations with host tree characteristics and environmental conditions. Flora 209:100–019Google Scholar
  24. de la Rosa-Manzano E, Andarade JL, García-Mendosa E, Zotz G, Reyes-García C (2015) Photoprotection related to xantophyll cycle pigments in epiphytic orchids acclimated at different microevironments in two tropical dry forests of the Yucatan Penisula, Mexico. Planta 242:1425–1438CrossRefPubMedGoogle Scholar
  25. Devlin PF, Christie JM, Terry MJ (2007) Many hands make light work. J Exp Bot 58(12):3071–3077CrossRefPubMedGoogle Scholar
  26. Dewi K, Purwestri YA, Astuti YTM, Natasaputra L, Parmi (2014) Effects of light quality on vegetative growth and flower initiation in Phalaenopsis. Indonesian J Biotech 19(1):33–42Google Scholar
  27. Dutta Gupta S, Jatothu B (2013) Fundamentals and applications of light-emitting diodes (LEDs) in in vitro plant growth and morphogenesis. Plant Biotech Rep 7:211–220CrossRefGoogle Scholar
  28. Ercole E, Rodda M, Molinatti M, Voyron S, Perotto S, Girlanda M (2013) Cryopreservation of orchid mycorrhizal fungi: a tool for conservation of endangered species. J Micr Meth 93:134–137CrossRefGoogle Scholar
  29. Fay MF (2015) British and Irish orchids in a changing world. Curtis’s Bot Mag 2(1):3–23CrossRefGoogle Scholar
  30. Folta KM, Maruhnich SA (2007) Green light: a signal to slow down or stop. J Exp Bot 58:3099–3111CrossRefPubMedGoogle Scholar
  31. Fracchia S, Aranda-Rickert A, Flachsland E, Terada G, Sede S (2014) Mycorrhizal compatibility and symbiotic reproduction of Gaviela australis, an endangered terrestial orchid from south Patagonia. Mycorrhiza 24:627–634CrossRefPubMedGoogle Scholar
  32. Freudenstein JV, Chase MW (2015) Phylogenetic relationships in Epidendroideae (Orchideaceae), one of the great flowering plant radiations: progressive specialization and diversification. Ann Bot 115:665–681CrossRefPubMedPubMedCentralGoogle Scholar
  33. Godo T, Fujiwara K, Guan K, Miyoshi K (2011) Effects of wavelength of LED-light on in vitro asymbiotic germination and seedling growth of Bletilla ochracea Schltr. (Orchidaceae). Plant Biotech 28:397–400CrossRefGoogle Scholar
  34. Gogoi K (2005) The genus Dendrobium in Dibru-Saikhowa National Park and Biosphere Reserve. J Orchid Soc India 19(1/2):17–25Google Scholar
  35. Gogoi K, Borah RL, Shrama GC (2010) Orchid flora of Dibru-Saikhowa National Park and Biosphere Reserve, Assam India. Pleione 4(1):124–134Google Scholar
  36. Habiba SU, Shimasaki K, Ahasan MM, Alam MM (2014a) Effect of 6-benzylaminopurine (BA) and hyaluronic acid (HA) under white light emitting diode (LED) on organogenesis in protocorm-likebodies (PLBs) of Dendrobium kingianum. American-Eurasian J Agric Environ Sci 14(7):605–609Google Scholar
  37. Habiba SU, Shimasaki K, Ahasan MM, Alam MM (2014b) Effects of different light quality on growth and development of protocorm-like bodies (PLBs) in Dendrobium kingianum cultured in vitro. Bangladesh Res Public J 10(2):223–227Google Scholar
  38. Hossain MM, Rahi P, Gulati A, Shrama M (2013) Improved ex vitro survival of asymbiotically raised seedlings of Cymbidium using mycorrhizal fungi isolated from distant orchid taxa. Sci Hortic 159:109–112CrossRefGoogle Scholar
  39. Hsu HC, Chen C (2010) The effect of light spectrum on the growth characteristics of in vitro cultures of Phalaenopsis. Prop Ornam Plants 10(1):3–8Google Scholar
  40. Jakubowska-Gabara J, Kurzac M, Kiedrzyński M, Kopeć D, Kucharski L, Kołodziejek J, Niedźwiedzki P, Popkiewicz P, Witosławski P, Zielińska K (2012) New stations of rare, protected and threatened species of vascular plants in Central Poland. Part II Fragm Florist Geobot 19(2):349–359Google Scholar
  41. Kaewjampa N, Shimasaki K (2012) Effects of green LED lighting on organogenesis and superoxide dismutase (SOD) activities in protocorm-like bodies (PLBs) of Cymbidium cultured in vitro. Environ Control Biol 50(3):247–254CrossRefGoogle Scholar
  42. Khamchatra N, Dixon KW, Taniwiwat S, Piapukiew J (2016) Symbiotic seed germination of an endangered epiphytic slipper orchid, Paphiopedillum villosum (Lindl.) Stein. from Thailand. S Afr J Bot 104:76–81CrossRefGoogle Scholar
  43. Kim SJ, Hahn EJ, Heo JW, Paek KY (2004) Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro. Sci Hortic 101:143–151CrossRefGoogle Scholar
  44. Knudson L (1946) A new nutrient medium for germination of orchid seeds. Am Orchid Soc Bull 39:214–217Google Scholar
  45. Křenová Z, Kidlmann P (2015) Natura 2000—solution for Eastern Europe or just a good start? The Šumava National Park as a test case. Biol Conserv 186:287–275Google Scholar
  46. Kull T, Selgis U, Peciῆa MV, Metsare M, Ilves A, Tali K, Sepp K, Kull K, Shefferson RP (2016) Factors influencing threat levels to orchids across Europe on the basis of national red lists. Ecol Evol 6(17):6245–6265CrossRefPubMedPubMedCentralGoogle Scholar
  47. Kurilčik A, Dapkūniene S, Kurličik G, Žilinskaite S, Žukauskas A, Duchovskis P (2008) Effect of photoperiod duration on the growth of Chrysanthemum plantlets in vitro. Sci Works Lith Inst Hort Lith Univ Agric Sodininkyste Ir Darzyninkyste 27(2):39–43Google Scholar
  48. Lemay MA, De Vriendt L, Pellerin S, Poulin M (2015) Ex situ germination as a method for seed viability assessment in a peatland orchid Palanthera blephariglottis. Am J Bot 102(3):390–395CrossRefPubMedGoogle Scholar
  49. Li H, Tang C, Xu Z (2013) The effects of different qualities on rapeseed (Brassica napus L.) plantlet growth and morphogenesis in vitro. Sci Hortic 150:117–124CrossRefGoogle Scholar
  50. Lin Y, Li J, Li B, He T, Chun Z (2011) Effects of light quality on growth and development of protocorm-like bodies of Dendrobium officinale in vitro. Plant Cell Tissue Organ Cult 105:329–335CrossRefGoogle Scholar
  51. Luan YQ, Huy NP, Nam NB, Huong TT, Hien FT, Hien NTT, Hai NT, Thinh DK, Nhut DT (2015) Ex vitro and in vitro Paphiopedilum delenatii Guillaumin stem elongation under light-emitting diodes and shoot regeneration via stem node culture. Acta Physiol Plant 37. doi: 10.1007/s11738-015-1886-8
  52. Martin KP, Madsssery J (2006) Rapid in vitro propagation of Dendrobium hybrids through direct shoot formation from foliar explants, and protocorm-like bodies. Sci Hortic 108:95–99CrossRefGoogle Scholar
  53. Mengxi L, Zhigang X, Yang Y, Yijie F (2011) Effects of different spectral lights on Oncidium PLBs induction, proliferation, and plant regeneration. Plant Cell Tiss Organ Cult 106:1–10CrossRefGoogle Scholar
  54. Mohanthy P, Paul S, Das MC, Kumaria S, Tandon P (2012) A simple and efficient protocol for the mass propagation of Cymbidium maestrii: an ornamental orchid of Northeast India. AoB Plants pls023. doi: 10.1093/aobpl/pls023
  55. Morrow RC (2008) LED lighting in horticulture. HortScience 43(7):1947–1950Google Scholar
  56. Müller R, Nowicki C, Barthlott W, Ibish PL (2003) Biodiversity and endemism maping as a tool for regional conservation planning—case study of the Pleurothallidinae (Orchidaceae) of the Andean rain forests in Bolivia. Biodiv Conserv 12:2005–20024CrossRefGoogle Scholar
  57. Nahar SJ, Haque SM, Kazuhiko S (2016) Application of chondroitin sulfate on organogenesis of two Cymbidium spp. under different sources of lights. Not Sci Biol 8(2):156–160Google Scholar
  58. Naing AH, Chung JD, Park IS, Lim KB (2011) Efficient plant regeneration of the endangered medicinal orchid, Colelogyne cristata using protocorm-like bodies. Acta Physiol Plant 33:659–666CrossRefGoogle Scholar
  59. Nayak NR, Rath SP, Patnaik S (1997) In vitro propagation of three epiphytic orchids, Cymbidium aloifolium (L.) Sw., Dendrobium aphyllum (Roxb.) Fisch. and Dendrobium moschatum (Buch-Ham) Sw. through thidiazuron-induced high frequency shoot proliferation. Sci Hortic 71:243–250CrossRefGoogle Scholar
  60. Nayak NR, Chand PK, Rath SP, Patnaik SN (1998) Influence of some plant growth regulators on the growth and organogenesis of Cymbidium aloifolium (L.) Sw. seed-derived rhizomes in vitro. In Vitro Cell Dev Biol Plant 34:185–188CrossRefGoogle Scholar
  61. Nayak NR, Sahoo S, Patnaik S, Rath SP (2002) Establisment of thin cross section culture (TCS) culture method for rapid micropropagation of Cymbidium aloifolium (L.) Sw and Dendrobium nobile Lind. (Orchidaceae). Sci Hortic 94:107–116CrossRefGoogle Scholar
  62. Ng ChY, Saleh NM (2011) In vitro propagation of Paphiopedilum orchid through formation of protocorm-like bodies. Plant Cell Tissue Organ Cult 105:193–202CrossRefGoogle Scholar
  63. Nordström S, Hedrén M (2009) Evolution, phylogeography and taxonomy of allopolyploid Dactylorhiza (Orchidaceae) and its implications for conservation. Nordic J Bot 27:458–556Google Scholar
  64. Ouzounis T, Rosenqvist E, Ottosen CO (2015) Spectral effects of artificial light on plant physiology and secondary metabolism: a review. HortScience 50(8):1128–1135Google Scholar
  65. Palomo I, Martīn-Lόpez B, Potschin M, Hainez-Young H, Montes C (2013) National parks, buffer zones and surrounding lands: mapping ecosystem service flows. Ecosyst Serv 4:104–116CrossRefGoogle Scholar
  66. Pan X, Zhou H, Li X, Wang W, Huang H (2015) LED mixed lighting for tissue culture of orchids. United States Patent No.: US 8,944,631 B2, 3 Feb 2015Google Scholar
  67. Panwar D, Ram K, Shekhwat NS (2012) In vitro propagation of Eulophia nuda Lindl., an endangered orchid. Sci Hortic 139:46–52CrossRefGoogle Scholar
  68. Park SY, Yeung EC, Paek KY (2010) Endoreduplication in Phalaenopsis is affected by light quality from light-emitting diodes during somatic embryogenesis. Plant Biotech Rep 4:303–309CrossRefGoogle Scholar
  69. Pindel A, Pindel Z (2004) Initiation of in vitro cultures of chosen endangered species of orchids. Folia Hortic 16(2):111–117Google Scholar
  70. Prahdan S, Tiwura B, Subedee BR, Pant B (2014) In vitro germination and propagation of a threatened medicinal orchid, Cymbidium aloifolium (L.) Sw. through artificial seed. Asian Pac J Trop Biomed 4(12):971–976Google Scholar
  71. Ramah S, Mubbarakh SA, Sinniah UR, Subramaniam S (2015a) Effect of droplet-vitrification on Brassidium Shooting Star’s orchid protocorm-like bodies (PLBs). Sci Hortic 197:254–260CrossRefGoogle Scholar
  72. Ramah S, Mubbarakh SA, Ping KS, Subramaniam S (2015b) Effects of dropled-vitrification cryopreservation based on physiological and antioxidant enzyme activities of Brassidium shooting star orchid. Sci World J. doi: 10.1155/2015/961793
  73. Rewicz A, Zielińska K, Kiedrzyński M, Kucharski L (2015) Orchidaceae in the antropogenic landscape of central Poland: diversity, extinction and conservation perspectives. Arch Biol Sci 67(1):119–130CrossRefGoogle Scholar
  74. Rudall PJ, Perl CD, Bateman RM (2012) Organ homologies in orchid flowers re-interpreted using the Musk Orchid as a model. PeerJ 1:e26. doi: 10.7717/peerj.26
  75. Saiprasad GVS, Raghuveer P, Khetarpal S, Chandra R (2004) Effect of various polyamines on production of protocorm-like bodies in orchid—Dendrobium ‘Sonia’. Sci Hort 100:161–168CrossRefGoogle Scholar
  76. Sathiyadash K, Muthukumar T, Murgan SB, Sathishkumar R, Pandey RR (2014) In vitro symbiotic seed germination of South Indian endemic orchid Coelogyne nervosa. Mycoscience 55:183–189CrossRefGoogle Scholar
  77. Seaton PT, Pritchard HW, Marks TR (2015) Aspects of orchid conservation: seed and polen storage and their value in re-introduction projects. Univ J Plant Sci 3(4):72–76CrossRefGoogle Scholar
  78. Seeni S, Latha PG (1992) Foliar regeneration of the endangered Red Vanda, Renanthera imschootiana Rolfe (Orchidaceae). Plant Cell Tissue Organ Cult 29:167–172CrossRefGoogle Scholar
  79. Sheshukova L, Klimenko E, Miryugina T, Olshetyn A, Vychuzhanina A (2014) Ecotourism in Western Siberia: issues and topical solutions. Middle-East J Sci Res 19(1):105–109Google Scholar
  80. Shimasaki K, Uemoto S (1990) Micropropagation of terrestial Cymbidium species using rhizomes developed from seeds and pseudobulbs. Plant Cell Tissue Organ Cult 22:237–244CrossRefGoogle Scholar
  81. Shin KS, Murthy HN, Heo JW, Hahn EJ, Paek KY (2008) The effect of light quality on the growth and development of in vitro cultured Doritaenopsis plants. Acta Physiol Plant 30:339–343CrossRefGoogle Scholar
  82. Singh D, Basu C, Meinhardt-Wollweber M, Roth B (2015) LEDs for energy efficient greenhouse lighting. Renew Sustain Energ Rev 49:139–147CrossRefGoogle Scholar
  83. Steward J, Griffiths M (1995) Manual of orchids. Timber Press, New YorkGoogle Scholar
  84. Swangmaneecharern P, Serivichyaswat P, Nontachaiyapoom S (2012) Promoting effect of orchid mycorrhizal fungi Epulorhiza isolates on seed germination of Dendrobium orchids. Sci Hortic 148:55–58CrossRefGoogle Scholar
  85. Swiderski A, Cybularz-Urban T, Hanus-Fajerska E (2007) The influence of UV radiation on the level of phenolic compounds in Cattleya leaves. Acta Physiol Plant 29(1 suppl.):115Google Scholar
  86. Tanaka M, Takamura T, Watanabe H, Endo M, Yanagi T, Okamoto K (1998) In vitro growth of Cymbidium plantlets cultured under superbright red and blue light-emitting diodes (LEDs). J Hortic Sci Biotech 73:39–44CrossRefGoogle Scholar
  87. Tokuhara K, Mii M (1993) Micropropagation of Phalenopsis and Doritaneopsis by culturing shoot tips of flower stalk buds. Plant Cell Rep 13:7–11CrossRefPubMedGoogle Scholar
  88. Tsai CC, Peng CI, Huang SC, Pl Huang, Chou Ch (2004) Determination of genetic relationship of Dendrobium species (Orchidaceae) in Taiwan based on the sequence of the internal transcribed spacer of ribosomal DNA. Sci Hortic 101:315–325CrossRefGoogle Scholar
  89. Vidal OJ, san Martin C, Mardones S, Bauk V, Vidal CF (2012) The orchids of Torres del Paine biosphere reserve: the need for species monitoring and ecotourism planning for biodiversity conservation. Gayana Bot 69(1):136–146Google Scholar
  90. Whigham DF, O’Neil JP, Rasmussen HN, Caldwell BA, McCormick MK (2006) Seed longevity in terrestial orchids—potential for persistent in situ seed banks. Biol Conserv 129:24–30CrossRefGoogle Scholar
  91. Wojciechowska R, Długosz-Grochowska O, Kołton A, Żupnik M (2015) The effects of LED supplemental lighting on yield and some quality parameters of lamb’s lettuce in two winter cycles. Sci Hortic 187:80–86CrossRefGoogle Scholar
  92. Wongnok A, Piluek C, Techasilpitak T, Tantivivat S (2008) Effects of light emitting diodes on micropropagation of Phalaenopsis orchids. Acta Hortic 788:149–156CrossRefGoogle Scholar
  93. Wu K, Zeng S, Lin D, da Silva JAT, Bu Z, Zhang J, Duan J (2014) In vitro propagation and reintroduction of the endangered Renanthera imschootiana Rolfe. PLoS One 9(10):e110033. doi: 10.1371/journal.pone0110033 CrossRefPubMedPubMedCentralGoogle Scholar
  94. Yano A, Fujiwara K (2012) Plant lighting system with five wavelength-band light-emitting diodes providing photon flux density and mixing ratio control. Plant Meth 8:46CrossRefGoogle Scholar
  95. Zang Z, Yan Y, Li J, He JS, Tang Z (2015) Distribution and conservation of orchid species richness in China. Biol Conserv 181:64–72CrossRefGoogle Scholar
  96. Zettler LW, Hofer CJ (1998) Propagation of the little club-spur orchid (Palanthera clavellata) by symbiotic seed germination and its ecological implications. Environ Exp Bot 39:189–195CrossRefGoogle Scholar
  97. Zhao P, Wu F, Feng FS, Wang WJ (2008) Protocorm-like body (PLB) formation and plant regeneration from the callus culture of Dendrombium candidum Wall ex Lindl. In Vitro Cell Dev Biol Plant 44:178185Google Scholar
  98. Zhao D, Hu G, Chen Z, Shi Y, Zheng L, Tang A, Long C (2013) Micropropagation and in vitro flowering of Dendrobuim wangliangii: a critically endangered medicinal orchid. J Med Plant Res 7(28):2098–2110CrossRefGoogle Scholar
  99. Zotz G (2013) The systematic distribution of vascular epiphytes—a critical update. Bot J Linn Soc 171:453–481CrossRefGoogle Scholar
  100. Zotz G, Winkler U (2013) Aerial roots of epiphytic orchids: the velamen radicum and its role in water and nutrient uptake. Oecologia 171:733–741CrossRefPubMedGoogle Scholar

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© Springer Nature Singapore Pte Ltd. 2017

Authors and Affiliations

  1. 1.Unit of Botany and Plant PhysiologyInstitute of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of AgricultureKrakówPoland

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