Abstract
Lignocellulose-to-ethanol conversion is a promising technology to supplement starch based ethanol production. Prosopis juliflora, a problematic weed has been recently suggested as one of the alternative lignocellulosic biomass materials for cellulosic ethanol production. Sodium hydroxide (NaOH) pretreatment performed at 100, 120 and 140 °C in an autoclave at 15 psi, with combination of residence times (15, 30, and 60 min) and NaOH concentrations (1, 2 and 3%) indicated that almost 51% of solids were dissolved at 140 °C after 60 min pretreatment with 3% NaOH concentration. The corresponding maximum lignin reductions of 48.39, 67.01 and 74.79% were obtained at 100, 120 and 140 °C respectively for 1 h, 3.0% NaOH concentrations. Hydrolysis was carried out with CTec2® Cellulase enzyme at different loading levels (0, 15 and 30%) and the results showed that the maximum rate of saccharification (26.07 mg/g/h) was attained at 12 h for sample pretreated at 120 °C, 60 min, 2% NaOH loaded with 30% enzyme with a total maximum sugar yield of 583.9 mg/g and the carbohydrate conversion of 90.86%. Batch fermentations of enzymatic hydrolyzates carried out with 5 g/l Saccharomyces cerevisiae at 30 °C indicated that fermentation of 46.71 g sugar/l sample resulted in maximum ethanol of 21.84 g/l with a productivity of 0.91 g/l/h and an ethanol yield of 0.27 g/g dry biomass.
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References
Alriksson B (2006) Ethanol from lignocellulose: alkali detoxification of dilute-acid spruce hydrolysates. Licentiate thesis, Karlstad University Studies, p 30
Bothast RJ, Saha BC (1997) Ethanol production from agricultural biomass substrates. Adv Appl Microbiol 44:261–286
Choge SK, Pasiecznik NM, Harvey M, Wright J, Awan SZ, Harris PJ (2007) Prosopis pods as human food, with special reference to Kenya. Nairobi Kenya For Res Inst 33(3):419–424. http://www.wrc.org.za
Eckard AD, Muthukumarappan K, Gibbons W (2012) Pretreatment of extruded corn stover with polyethylene glycol to enhance enzymatic hydrolysis: optimization, kinetics, and mechanism of action. Bioenergy Res 5:424–438
Eylen DV, Femke VD, Kabel M, Bont J (2011) Corn fiber, cobs and stover: enzyme-aided saccharification and co-fermentation after dilute acid pretreatment. Bioresour Technol 102:5995–6004
Ghose TK (1987) Measurement of cellulase activities. Pure Appl Chem 59:257–268
Gupta R, Sharma KK, Kuhad RC (2009) Separate hydrolysis and fermentation (SHF) of Prosopis juliflora, a woody substrate, for the production of cellulosic ethanol by Saccharomyces cerevisiae and Pichia stipitis -NCIM 3498. Bioresour Technol 100:1214–1220
Hopkins M (2007) Cooking up a smoky solution. Nature News, doi:10.1038/news070813-1, News
Kodaganti BP (2011) Simultaneous saccharification and fermentation of Arundo donax—comparison of feeding strategies. www.chemeng.lth.se/E655.pdf. Accessed 24 May 2012
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal Chem 31:426–428
Mosier N, Wyman C, Dale B, Elander R, Lee YY, Holtzapple M, Ladisch M (2005) Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresour Technol 96(6):673–686
Rajput SS, Tewari MC (1986) The role of prosopis in wasteland development. In: Patel VJ (ed) Javrajbhai Agroforestry Center, Surendrabag, Gujarat, India
Selig M, Weiss N, Ji Y (2008) Enzymatic saccharification of lignocellulosic biomass. Laboratory analytical procedure (LAP). National Renewable Energy Laboratory, Golden
Sharma R, Palled V, Sharma-Shivappa RR, Osborne Jason (2013) Potential of potassium hydroxide pretreatment of switchgrass for fermentable sugar production. Appl Biochem Biotechnol 169:761–772
Silverstein RA, Chen Y, Sharma-Shivappa RR, Boyette MD, Osborne J (2007) A comparison of chemical pretreatment methods for improving saccharification of cotton stalks. Bioresour Technol 98:3000–3011
Sluiter A, Hames B, Hyman D, Payne C, Ruiz R, Scarlata C, Sluiter J, Templeton D, Wolfe J (2005a) Determination of total solids in biomass and total dissolved solids in liquid process samples. Laboratory Analytical Procedure (LAP). National Renewable Energy Laboratory, Golden
Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D (2008) Determination of structural carbohydrates and lignin in biomass. Laboratory Analytical Procedure (LAP). National Renewable Energy Laboratory, Golden
Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D (2005b) Determination of ash in biomass. Laboratory Analytical Procedure (LAP). National Renewable Energy Laboratory, Golden
Sun Y, Cheng J (2002) Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresour Technol 83:1–11
Thuesombat P, Thanonkeo P, Laopaiboon L, Laopaiboon P, Yunchalard S, Kaewkannetra L, Thanonkeo S (2007) The batch ethanol fermentation of jerusalem artichoke using saccharomyces cerevisiae. J Sci Technol 7:93–96
Xu J, Cheng JJ, Sharma-Shivappa RR, Burns JC (2010) Sodium hydroxide pretreatment of switchgrass for ethanol production. Energy Fuels 24:2113–2119
Acknowledgements
The authors would like to acknowledge the training imparted by Dr. Ratna Sharma-Shivappa, Associate Professor, Department of Biological and Agricultural Engineering, North Carolina State University, Raleigh, USAon “Fermentation Technology”. Further, the authors would like to extend sincere thanks to the Novozymes, Beijing, China for sponsoring CTec2® Cellulase enzyme complex for conducting research on hydrolysis.
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Palled, V. et al. (2018). Prosopis juliflora—A Potential Problematic Weed for Lignocellulosic Ethanol Production. In: Singh, V., Yadav, S., Yadava, R. (eds) Energy and Environment. Water Science and Technology Library, vol 80. Springer, Singapore. https://doi.org/10.1007/978-981-10-5798-4_19
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