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Metagenomic Insights into Microbial Diversity and Metabolic Potential of Hot Spring Ecosystems

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Abstract

Hot water springs or hydrothermal springs are places where warm or hot water comes out of earth surfaces regularly or for a significant period, in a year. These ecosystems present an epitome of extreme environments and are extensively distributed all over the globe. Geographically, these ecosystems encompass unique physical and chemical characteristics. Interestingly, 16S rRNA gene analysis in combination with next-generation sequencing has provided in-depth knowledge about phylogeny and the metabolic potential of a particular environment, including the hot springs. Every hot spring is unique and dynamic in its characteristics compare to the other. Investigation of metagenome from diverse ecological habitats, using high-throughput sequencing or library construction, has led to the discovery of a number of novel biocatalysts. Metagenomic studies in recent years have achieved two major goals: first it has resulted in deep understanding about structural and functional dynamics of microbial communities, and secondly, it has led to the discovery of diverse novel bioactive molecules. This book chapter will shed light into the role of metagenome gene cloning in revealing the true and comprehensive diversity and the metabolic potential of microbes in hot spring ecosystems.

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Acknowledgement

Financial assistance to Dr. P.K.S. by the Science and Engineering Research Board (SERB) New Delhi, with project file number: SB/YS/LS-63/2013, under fast track scheme for the young scientists, is highly acknowledged.

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  1. Department of Biotechnology, Sri Guru Granth Sahib World University, Fatehgarh Sahib, Punjab, 140 406, India

    Jasleen Saini, Ramanpreet Kaur & Pushpender Kumar Sharma Ph.D.

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  1. Jasleen Saini
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  2. Ramanpreet Kaur
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  3. Pushpender Kumar Sharma Ph.D.
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Correspondence to Pushpender Kumar Sharma Ph.D. .

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  1. Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology, New Delhi, India

    Vipin Chandra Kalia

  2. Microbial Culture Collection, National Centre for Cell Science, Pune, India

    Yogesh Shouche

  3. Environmental Biotechnology and Genomics Division, National Environmental Engineering Research Institute (CSIR), Nagpur, India

    Hemant J. Purohit

  4. Microbial Culture Collection, National Centre for Cell Science, Pune, India

    Praveen Rahi

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Saini, J., Kaur, R., Sharma, P.K. (2017). Metagenomic Insights into Microbial Diversity and Metabolic Potential of Hot Spring Ecosystems. In: Kalia, V., Shouche, Y., Purohit, H., Rahi, P. (eds) Mining of Microbial Wealth and MetaGenomics. Springer, Singapore. https://doi.org/10.1007/978-981-10-5708-3_9

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