Abstract
The quality and quantity of rotifer production in a hatchery primarily determine the yield of larval production; therefore, considerable studies geared toward mass culture and preservation of rotifers. Rotifer mass production, attaining ultrahigh density and greater culture stability, was attained when condensed microalgal pastes (Chlorella and Nannochloropsis) were introduced and used in a closed recirculation system. The rotifer culture health status can be determined by measuring water quality, rotifer physiological conditions such as egg ratio, swimming speed, ingestion rate, and in vivo enzyme that takes 1–2 days to finish. Addition of chemicals and hormones such as γ-aminobutyric acid (GABA), porcine growth hormone, serotonin, and human chorionic gonadotropin is known to improve the health status of cultured rotifers. Mass-produced rotifers can be preserved at low temperature; these rotifers can either be directly used as feed for the larvae or as starters of rotifer culture. For long-term storage, collection and storage of resting eggs are recommended. For species which do not produce resting eggs, cryopreservation of amictic eggs is recommended.
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Hagiwara, A., Kim, HJ., Marcial, H. (2017). Mass Culture and Preservation of Brachionus plicatilis sp. Complex. In: Hagiwara, A., Yoshinaga, T. (eds) Rotifers. Fisheries Science Series. Springer, Singapore. https://doi.org/10.1007/978-981-10-5635-2_3
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