Molecular Characterization of Anogeissus acuminata Genotypes Employing RAPD Markers

Abstract

Anogeissus acuminata (Roxb. ex DC.) Wall. ex Guill. & Perr.** is commonly known as button tree and locally Phasi/Pasi. It is the specific kind of tree species with spiritual and cognitive environs of Odisha as its timber is useful in famous Ratha Yatra to make chariot wheels of Lord Jagannatha. Due to its potency and efficient qualities; Anogeissus timber is of implicit value, including its medicinal and economical values. The present work was aimed at studying the genetic diversity and relationship existing among the genotypes and discrimination at the inter-specific and intra-specific level, employing RAPD markers. Using 22 primers, 166 distinct bands were generated out of which 106 bands (66.63%) were found to be polymorphic. The amplicons obtained after PCR were run on 1.5% gel and was visualized under gel documentation system. The timber availability has become a challenge during the festival every year. So, it is necessary to conserve this species for future generations. Thus, genetic diversity and molecular characterization assessment through DNA-based molecular markers may be reliable and authentic for the species. In plant breeding programmes the most diverse genotypes shall be used in the hybridization programme.