Skip to main content

Phytochemical Screening and Antioxidant Property of Anthocyanins Extracts from Hibiscus rosa-sinensis

  • Conference paper
  • First Online:
Applications of Biotechnology for Sustainable Development

Abstract

This study was aimed at investigating the antioxidant property of anthocyanin extracts (AEs) from Hibiscus rosa-sinensis (petal). Preliminary phytochemical studies confirmed the presence of tannins, saponins, alkaloids, steroids, flavonoids and carbohydrates in the extract. Total phenolic content (TPC), total flavonoid content (TFC) and total anthocyanin content (TAC) of the AEs were also determined. In vitro antioxidant activity of the AEs was assessed by nitric oxide (NO) scavenging assay. The AEs demonstrated efficient and dose dependent inhibition of nitric oxide. A strong correlation was observed between antioxidant activity when compared with TPC, TFC and TAC. This confirmed that phenolics, flavonoids and anthocyanins were responsible for the exhibited antioxidant activity. Thus, this study highlighted H. rosa-sinensis having antioxidant properties which may be due to its anthocyanins, phenolics and flavonoids contents.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Andersen ØM, Markham K (2006) Anthocyanins. In: Flavonoids: chemistry, biochemistry, and applications, CRC Press, Boca Raton

    Google Scholar 

  • Bhaskar A, Nithya V, Vidhya VG (2011) Phytochemical screening and in-vitro antioxidant activities of ethanolic extracts of Hibiscus rosa-sinensis L. Ann Bio Res 2:653–661

    CAS  Google Scholar 

  • Bueno JM, Ramos-Escudero F, Sáez-Plaza P, Muñoz A, JoséNavas M, Asuero AG (2012) Analysis and antioxidant capacity of anthocyanin pigments. Part I: general considerations concerning polyphenols and flavonoids. Cr Rev Anal Chem 42:102–125

    Article  CAS  Google Scholar 

  • Chia LS, Goh NK, Chia TF, Brouillard R, Jin-Ming Konga (2003) Analysis and biological activities of anthocyanins. Phytochemistry 64:923–933

    Article  PubMed  Google Scholar 

  • Faten R, Ghaffar A, El-Elaimy I (2012) In-vitro, antioxidant and scavenging activities of Hibiscus rosa-sinensis crude extract. J App Pharm Sci. 2:51–58

    Google Scholar 

  • Giusti MM, Wrolstad RE (2001) Characterization and measurement of anthocyanins by UV-visible spectroscopy. In: Current protocols in food analytical chemistry, Wiley, NY, pp 1–13

    Google Scholar 

  • Harman D (1956) Ageing: a theory based on free radical and radiation chemistry. J Gerontol 11:298–300

    Article  CAS  PubMed  Google Scholar 

  • Halliwell B, Gutteridge JMC (1997) Free radicals in biology and medicine. Oxford University Press, Oxford

    Google Scholar 

  • Kong JM, Chia LS, Goh NK, Chia TF, Brouillard R (2003) Analysis and biological activities of anthocyanins. Phytochemistry 64:923–933

    Google Scholar 

  • Kumar A, Singh A (2012) Review on Hibiscus rosa-sinensis. Int J Res Pharm Biomed Sci 3:2229–3701

    Google Scholar 

  • Lee J, Kim H, Kim J, Jang Y (2007) Antioxidant property of an ethanol extract of the stem of Opuntia ficus-indica var. Saboten. J Agric Food Chem 50:6490–6496

    Google Scholar 

  • Liu X, Zhao M, Wang J, Yang B, Jiang Y (2008) Antioxidant activity of methanolic extract of emblica fruit (Phyllanthus emblica L.) from six regions in China. J Food Compos Anal 21:219–228

    Article  Google Scholar 

  • Moncada A, Palmer RMJ, Higgs EA (1991) Nitric oxide: physiology, pathology and pharmacology. Pharm Rev 43:109–142

    CAS  PubMed  Google Scholar 

  • Patel A, Patel A, Patel NM (2010) Determination of polyphenols and free radical scavenging activity of Tephrosia purpurea L. leaves (Leguminosae). Pharmacog Res 2:152–158

    Article  CAS  Google Scholar 

  • Prasad MP (2014) In vitro phytochemical analysis and antioxidant studies of Hibiscus species. Int J Pure App Bio 2:83–88

    Google Scholar 

  • Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Amm J Enol Viti 16:144–158

    CAS  Google Scholar 

  • Soto-Vaca A, Gutierrez A, Losso JN, Xu Z, Finley JW (2012) Evolution of phenolic compounds from colour and flavour problems to health benefits. J Agric Food Chem 60:6658–6677

    Article  CAS  PubMed  Google Scholar 

  • Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byrne DH (2006) Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Comp Anal 19:669–675

    Article  CAS  Google Scholar 

  • Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H (2011) Phytochemical screening and extraction: a review. Int Pharm Sci 1:98–106

    Google Scholar 

  • Wang C, Wang J, Lin W, Chu C, Chou F, Tseng T (2000) Protective effect of Hibiscus anthocyanins against tert-butyl hydroperoxide-induced hepatic toxicity in rats. Food Chem Toxicol 38:411–416

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Biswatrish Sarkar .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer Nature Singapore Pte Ltd.

About this paper

Cite this paper

Anand, A., Sarkar, B. (2017). Phytochemical Screening and Antioxidant Property of Anthocyanins Extracts from Hibiscus rosa-sinensis . In: Mukhopadhyay, K., Sachan, A., Kumar, M. (eds) Applications of Biotechnology for Sustainable Development. Springer, Singapore. https://doi.org/10.1007/978-981-10-5538-6_17

Download citation

Publish with us

Policies and ethics