Abstract
This study was aimed at investigating the antioxidant property of anthocyanin extracts (AEs) from Hibiscus rosa-sinensis (petal). Preliminary phytochemical studies confirmed the presence of tannins, saponins, alkaloids, steroids, flavonoids and carbohydrates in the extract. Total phenolic content (TPC), total flavonoid content (TFC) and total anthocyanin content (TAC) of the AEs were also determined. In vitro antioxidant activity of the AEs was assessed by nitric oxide (NO) scavenging assay. The AEs demonstrated efficient and dose dependent inhibition of nitric oxide. A strong correlation was observed between antioxidant activity when compared with TPC, TFC and TAC. This confirmed that phenolics, flavonoids and anthocyanins were responsible for the exhibited antioxidant activity. Thus, this study highlighted H. rosa-sinensis having antioxidant properties which may be due to its anthocyanins, phenolics and flavonoids contents.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Andersen ØM, Markham K (2006) Anthocyanins. In: Flavonoids: chemistry, biochemistry, and applications, CRC Press, Boca Raton
Bhaskar A, Nithya V, Vidhya VG (2011) Phytochemical screening and in-vitro antioxidant activities of ethanolic extracts of Hibiscus rosa-sinensis L. Ann Bio Res 2:653–661
Bueno JM, Ramos-Escudero F, Sáez-Plaza P, Muñoz A, JoséNavas M, Asuero AG (2012) Analysis and antioxidant capacity of anthocyanin pigments. Part I: general considerations concerning polyphenols and flavonoids. Cr Rev Anal Chem 42:102–125
Chia LS, Goh NK, Chia TF, Brouillard R, Jin-Ming Konga (2003) Analysis and biological activities of anthocyanins. Phytochemistry 64:923–933
Faten R, Ghaffar A, El-Elaimy I (2012) In-vitro, antioxidant and scavenging activities of Hibiscus rosa-sinensis crude extract. J App Pharm Sci. 2:51–58
Giusti MM, Wrolstad RE (2001) Characterization and measurement of anthocyanins by UV-visible spectroscopy. In: Current protocols in food analytical chemistry, Wiley, NY, pp 1–13
Harman D (1956) Ageing: a theory based on free radical and radiation chemistry. J Gerontol 11:298–300
Halliwell B, Gutteridge JMC (1997) Free radicals in biology and medicine. Oxford University Press, Oxford
Kong JM, Chia LS, Goh NK, Chia TF, Brouillard R (2003) Analysis and biological activities of anthocyanins. Phytochemistry 64:923–933
Kumar A, Singh A (2012) Review on Hibiscus rosa-sinensis. Int J Res Pharm Biomed Sci 3:2229–3701
Lee J, Kim H, Kim J, Jang Y (2007) Antioxidant property of an ethanol extract of the stem of Opuntia ficus-indica var. Saboten. J Agric Food Chem 50:6490–6496
Liu X, Zhao M, Wang J, Yang B, Jiang Y (2008) Antioxidant activity of methanolic extract of emblica fruit (Phyllanthus emblica L.) from six regions in China. J Food Compos Anal 21:219–228
Moncada A, Palmer RMJ, Higgs EA (1991) Nitric oxide: physiology, pathology and pharmacology. Pharm Rev 43:109–142
Patel A, Patel A, Patel NM (2010) Determination of polyphenols and free radical scavenging activity of Tephrosia purpurea L. leaves (Leguminosae). Pharmacog Res 2:152–158
Prasad MP (2014) In vitro phytochemical analysis and antioxidant studies of Hibiscus species. Int J Pure App Bio 2:83–88
Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Amm J Enol Viti 16:144–158
Soto-Vaca A, Gutierrez A, Losso JN, Xu Z, Finley JW (2012) Evolution of phenolic compounds from colour and flavour problems to health benefits. J Agric Food Chem 60:6658–6677
Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byrne DH (2006) Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Comp Anal 19:669–675
Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H (2011) Phytochemical screening and extraction: a review. Int Pharm Sci 1:98–106
Wang C, Wang J, Lin W, Chu C, Chou F, Tseng T (2000) Protective effect of Hibiscus anthocyanins against tert-butyl hydroperoxide-induced hepatic toxicity in rats. Food Chem Toxicol 38:411–416
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Nature Singapore Pte Ltd.
About this paper
Cite this paper
Anand, A., Sarkar, B. (2017). Phytochemical Screening and Antioxidant Property of Anthocyanins Extracts from Hibiscus rosa-sinensis . In: Mukhopadhyay, K., Sachan, A., Kumar, M. (eds) Applications of Biotechnology for Sustainable Development. Springer, Singapore. https://doi.org/10.1007/978-981-10-5538-6_17
Download citation
DOI: https://doi.org/10.1007/978-981-10-5538-6_17
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-10-5537-9
Online ISBN: 978-981-10-5538-6
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)