Abstract
Sericulture or cultivation of silkworms is practiced in India and other Asian countries since time immemorial. The mulberry silkworm, Bombyx mori are affected by viral, fungal and protozoan pathogens, which causes huge monetary loss. Among many other diseases, Flacherie is the most rampant in the silkworm community causing flaccidity and subsequent death. Previous studies indicate Andrographolide, Quercitin and chitosan are the active compounds from the extracts of Andrographis paniculata and Tridax procumbens to be effective against the flacherie pathogens. The main aim of the following work depends on the screening and selecting of the potent inhibitor against the spore wall proteins of flacherie-infected B. mori. In this study, two wall proteins were selected from flacherie-infected mulberry silkworm, the 3D structures were modeled through Modeller 9.15. Eventually the modeled structures were docked with reference inhibitors (three best reference) using GLIDE suite of Maestro 9.3.5. The best one among the three references was identified and selected for the screening of next set of inhibitors (50 inhibitors) with a structural similarity of 70, 80 and 90%. They were again docked in the same active region of the targets and identified as the best reference inhibitor. The result shows a promising potent inhibitor which can be further validated by experimental procedures.
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Acknowledgements
The authors acknowledge BTISnet, Department of Biotechnology, Government of India, New Delhi and TEQIP-II, BIT Mesra for providing Infrastructure facility for the mentioned study. The authors also acknowledge Anand Boipai and Deepak Toppo for providing information for the mentioned study.
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Banerjee, D., Mukherjee, K. (2017). Identification and Screening of Potent Inhibitors Against Spore Wall Proteins of Flacherie Infected Bombyx mori Through Molecular Modeling and Docking Studies. In: Mukhopadhyay, K., Sachan, A., Kumar, M. (eds) Applications of Biotechnology for Sustainable Development. Springer, Singapore. https://doi.org/10.1007/978-981-10-5538-6_14
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DOI: https://doi.org/10.1007/978-981-10-5538-6_14
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