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Biochemical Assays in Spermatology

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Protocols in Semen Biology (Comparing Assays)

Abstract

Declaration of a male as fertile involves complete evaluation of sperm cell quality as well as that of seminal plasma. Qualitative and quantitative measurements of seminal contents yield valuable insight to arrive at a conclusive finding about cellular damages and functional status of accessory sex glands. This chapter dwells about measurements of acrosin, hyaluronidase and transaminases as a marker of sperm cell damages. Moreover, protocols related to determination of various enzymes and other seminal contents, viz. neutral α-glucosidase (for epididymis function) and acid phosphatase, ascorbic acids and zinc (for prostatic function), are described as a means to assess functional status of accessory sex glands. Estimation of seminal fructose as a means to evaluate functional status of seminal vesicle has been provided elsewhere.

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Key References

  1. WHO laboratory manual 2010. See above [23] In-depth discussion and excellent background information related to various biochemical assays for semen quality assessment.

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  2. Kumar and Singh LP 2003. See above [5] Provides procedures for several assay for estimation of enzymes.

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Author information

Authors and Affiliations

  1. Quality Control, Semen Freezing Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, 250001, Uttar Pradesh, India

    N. Srivastava

  2. Animal Physiology and Embryo Transfer Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, 250001, Uttar Pradesh, India

    Megha Pande

Authors
  1. N. Srivastava
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  2. Megha Pande
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Correspondence to N. Srivastava .

Editor information

Editors and Affiliations

  1. Quality Control, Semen Freezing Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, India

    N. Srivastava

  2. Animal Physiology and Embryo Transfer Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, India

    Megha Pande

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Srivastava, N., Pande, M. (2017). Biochemical Assays in Spermatology. In: Srivastava, N., Pande, M. (eds) Protocols in Semen Biology (Comparing Assays). Springer, Singapore. https://doi.org/10.1007/978-981-10-5200-2_9

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