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Protocols in Semen Biology (Comparing Assays) pp 43–55Cite as

Estimates of Sperm Motility

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Abstract

Spermatozoa motility is a very important attribute most commonly exploited for discriminating between a good and a bad semen sample at the fresh as well as post-cryopreservation stage. Accurate measurement of motility provides crucial information about viability of spermatozoa as well. In the chapter on sperm motility, principles governing various motility parameters, protocols and finer practical hints have been provided for the investigator to carry out experimentation accurately. We have provided protocols of estimating sperm motility using CASA for an effective and objective assessment as well. This section includes various advantages and disadvantages of CASA vis-à-vis other motility parameters as well.

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Literature Cited

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Key References

  1. Salisbury GW (1985) See above [3] In-depth discussion and excellent background information related to estimation of motility of spermatozoa

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  2. Kumar and Singh (2003) See above [4] For procedures related to routine semen evaluation

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  3. Edwin and Ulganathan (1988) See above [5] For background information and detailed description of routine semen evaluation

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  4. WHO laboratory manual, 5th edn (2010). See above [15] For excellent explanation of semen evaluation protocols including protocols and application of CASA

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Author information

Authors and Affiliations

  1. Quality Control, Semen Freezing Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, 250001, Uttar Pradesh, India

    N. Srivastava

  2. Animal Physiology and Embryo Transfer Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, 250001, Uttar Pradesh, India

    Megha Pande

Authors
  1. N. Srivastava
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  2. Megha Pande
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Corresponding author

Correspondence to N. Srivastava .

Editor information

Editors and Affiliations

  1. Quality Control, Semen Freezing Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, India

    N. Srivastava

  2. Animal Physiology and Embryo Transfer Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, India

    Megha Pande

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Srivastava, N., Pande, M. (2017). Estimates of Sperm Motility. In: Srivastava, N., Pande, M. (eds) Protocols in Semen Biology (Comparing Assays). Springer, Singapore. https://doi.org/10.1007/978-981-10-5200-2_5

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