Abstract
The practical advantage of an immunological methodology to the study of carbohydrates, proteins, and other cellular structures is that when carefully carried out, results are highly specific, sensitive, and repeatable. All immunological assays in semen biology involve measurement of the interaction of targeted antibody/antigen (Ab-Ag) with that of Ag-Ab. Since most of the investigations in the semen biology involve either quantitative or qualitative assay of interaction of Ab with Ag, we have divided such assays in two parts. Part one, the qualitative assays, includes the enzyme-linked immunosorbent assay (ELISA) with its four common variants, namely, indirect ELISA, direct competitive ELISA, antibody-sandwich ELISA, and radioimmunoassay (RIA). Part two, the quantitative assays, includes double immunodiffusion (DID), Western blot, and dot blot assays. Additionally, tests for screening of antisperm antibody (ASA) on the sperm cells as well as biological fluids have been explained. This chapter also includes isolation of Ab (specifically IgG) by chromatography method. The comparison of various assays has been provided in the relevant sections.
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Key References
Coligan and co-workers (1992) See above [3] Provides protocols and comparative merits/demerits of various ELISA and other techniques employed in assessment of interaction of binding of Ab with Ag.
WHO laboratory manual (2010) See above [6] Provides excellent information on some of the immunological assays mentioned in this chapter.
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Srivastava, N., Pande, M., Din, O. (2017). Immuno-Reproduction Assays in Semen Biology. In: Srivastava, N., Pande, M. (eds) Protocols in Semen Biology (Comparing Assays). Springer, Singapore. https://doi.org/10.1007/978-981-10-5200-2_19
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DOI: https://doi.org/10.1007/978-981-10-5200-2_19
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