Abstract
Proteins of seminal plasma or sperm membrane are important contributing factors in quality of fresh or modulated semen. Because of their significance and the beneficial or detrimental effect of spermatozoa quality, determining protein content becomes imperative to get a conclusive fertility report. This chapter outlines some classical protocols like salting out proteins as well as other general protocols like Lowry’s method, molecular sieve and ion-exchange chromatography as a guiding principle to isolate seminal proteins.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Literature Cited
Chacur MGM (2012) Seminal plasma proteins as potential markers of relative fertility in Zebu Bulls (Bos taurus-indicus). Electrophoresis:173–193
Kumar V, Hassan MI, Tomar AK, Kashav T, Nautiyal J, Singh S, Yadav S (2009) Proteomic analysis of heparin-binding proteins from human seminal plasma: a step towards identification of molecular markers of male fertility. J Biosci 34(6):899–908
Manjunath P, Thérien I (2002) Role of seminal plasma phospholipid-binding proteins in sperm membrane lipid modification that occurs during capacitation. J Reprod Immunol 53(1–2):109–119
Srivastava N, Srivastava SK, Ghosh SK, Singh LP, Prasad JK, Kumar A, Perumal P, Jerome A, Thamizharasan A (2012) Sequestration of PDC-109 protein improves freezability of crossbred bull spermatozoa. Anim Reprod Sci 131:54–62
King TP (1972) Separation of proteins by ammonium sulfate gradient solubilization. Biochemistry 11(3):367–371
Warburg and Christian (1941) Isolierung und Kristallisation des Garunghferments Enolase. Biochem J 310:384
Gornall AG, Bardawill CJ, David M (1949) Determination of serum proteins by means of the biuret reaction. J Biol Chem 177:751
Robinson HM (1984) The determination of serum proteins. J Biol Chem 135:707
Folin O, Ciacalteu C (1927) On tyrosine and tryptophane determinations in proteins. J Biol Chem 73:627
Lowry OH, Rosebrough NJ, Farr AB, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:165
Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Warren Strober, (1992) Current protocols in immunology. In: Richard Coico (ed) Current protocols. Wiley, Greene Publishing Associates, Inc. and John Willey and Sons, Inc. USA.
Therien I, Bleau G, Manjunath P (1995) Phosphatidylcholine-binding proteins of bovine seminal plasma modulates capacitation of spermatozoa by heparin. Biol Reprod 52:1372–1379
Killian GJ, Chapman DA, Rogowski LA (1993) Fertility-associated proteins in Holstein bull seminal plasma. Biol Reprod 49:1202–1207
Moura AA, Koc H, Chapmon DA, Killian GJ (2006) Identification of proteins in the accessory sex gland fluid associated with fertility indexes of dairy bulls: a proteomic approach. Androl J 27(2):201–211
Moura AA, Koc H, Chapman DA (2007) A comprehensive proteomic analysis of the accessory sex gland fluid from mature Holstein bulls. Anim Reprod Sci 98:169–188
Key Reference
Coligan and co-workers (1992) See above [11] Provides protocols and comparative merits/demerits of some of the chromatography techniques employed in isolation of proteins
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Nature Singapore Pte Ltd.
About this chapter
Cite this chapter
Srivastava, N., Pande, M. (2017). Estimating Proteins of Semen. In: Srivastava, N., Pande, M. (eds) Protocols in Semen Biology (Comparing Assays). Springer, Singapore. https://doi.org/10.1007/978-981-10-5200-2_16
Download citation
DOI: https://doi.org/10.1007/978-981-10-5200-2_16
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-10-5199-9
Online ISBN: 978-981-10-5200-2
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)