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Estimating Metabolic Activity of Spermatozoa

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Protocols in Semen Biology (Comparing Assays)

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Abstract

The motility of live spermatozoa indicating energy requirement and thus presence of intrinsic metabolic pathways in the cell has led researchers to speculate relationship of sperm metabolism with semen quality parameters. Over the period, many assays, some quantitative and some qualitative, to estimate metabolic aspects of sperm life have been developed. Investigators have reported significant relationship of metabolic assays with that of sperm concentrations and motility. Moreover, a significant relationship of resazurin reduction assay with that of oxidative stress of spermatozoa has also been reported recently. This chapter outlines principle and procedures involved in various assays employed for estimating metabolic rates of bovine spermatozoa. Modification of resazurin assay to objectively measure colour changes using spectrophotometer and, in fructose estimation, protocols to evaluate metabolic rates in sperm suspension and frozen-thawed samples have been included.

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Key References

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  2. Zalata et al. See above [16] Pointed out relationship of resazurin reduction rate with oxidative stress of spermatozoa

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  4. Anderson and co-workers. See above [22] This group of researchers have provided in-depth discussion of enzymatic assay to determine sperm metabolism, with stepwise description of related protocol

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Authors and Affiliations

  1. Quality Control, Semen Freezing Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, 250001, Uttar Pradesh, India

    N. Srivastava

  2. Animal Physiology and Embryo Transfer Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, 250001, Uttar Pradesh, India

    Megha Pande

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  1. N. Srivastava
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  2. Megha Pande
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Correspondence to N. Srivastava .

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Editors and Affiliations

  1. Quality Control, Semen Freezing Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, India

    N. Srivastava

  2. Animal Physiology and Embryo Transfer Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, India

    Megha Pande

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Srivastava, N., Pande, M. (2017). Estimating Metabolic Activity of Spermatozoa. In: Srivastava, N., Pande, M. (eds) Protocols in Semen Biology (Comparing Assays). Springer, Singapore. https://doi.org/10.1007/978-981-10-5200-2_10

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