Abstract
γ-polyglutamic acid (γ-PGA) is a kind of biopolymer with high accessional value, and yeast extract is a crucial raw material for the fermentation of γ-PGA. In this paper, the extraction of yeast extract form the waste beer yeast was optimized. Result showed that, 24-h autolysis at 55 °C with 2% NaCl, 2‰ dextranase and 3‰ complex protease addition, the yield of yeast extract was reached 48.64% (w/w). Then the yeast extract was used in γ-PGA fermentation, and the corresponding γ-PGA yield reached 18.69 g/L with 7.5% yeast extract addition, increased by 290% than that of without yeast extract addition.
Jun Yu, Lin Zhao and Song Li are contributed equally to this paper.
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Acknowledgements
The work was financially supported by Shandong Provincial Key Research and Development Program (No. 2015ZDZX05001, 2015ZDXX0502B01, 2015ZDXX0403B01, 2016GGX107003), National Natural Science Foundation (No. 31501396), Shandong Provincial Natural Science Foundation (No. ZR2012CQ027).
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Yu, J., Zhao, L., Li, S., Sun, X., Liu, Xl. (2018). Research on γ-Polyglutamic Acid Fermentation with Extract from Waste Beer Yeast. In: Liu, H., Song, C., Ram, A. (eds) Advances in Applied Biotechnology. ICAB 2016. Lecture Notes in Electrical Engineering, vol 444. Springer, Singapore. https://doi.org/10.1007/978-981-10-4801-2_82
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DOI: https://doi.org/10.1007/978-981-10-4801-2_82
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