Abstract
d-pantolactone is an important intermediate for the production of pantothenic acid, panthenol, and calcium d-pantothenate. It is an efficient method to obtain d-pantolactone by optical resolution of dl-pantolactone using d-lactonohydrolase. In order to improve the hydrolysis rate of dl-pantolactone using immobilized d-lactonohydrolase which showed excellent pH and temperature stability, the process for optical resolution of dl-pantolactone using immobilized d-lactonohydrolase was investigated by optimization of hydrolysis condition. The results showed that the optimum process for optical resolution of dl-pantolactone was: temperature 40°C, pH7.0, substrate concentration 20%, hydrolysis time 8 h. Under the optimum condition, the hydrolysis rate of dl-pantolactone reached 36.8%, and the hydrolysis rate still remained at 85.6% of the original hydrolysis rate after 20 batches of reuse, indicating that immobilized d-lactonohydrolase had excellent operational stability.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (No. 21176190). We thank NanKai University for providing the resin D-380 kindly.
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Xuan, Mj., Zhang, Jz., Yuan, Hy., Wei, R., Lu, Fp., Li, M. (2018). Optimization of Process for Optical Resolution of dl-Pantolactone Using Immobilized d-Lactonohydrolase. In: Liu, H., Song, C., Ram, A. (eds) Advances in Applied Biotechnology. ICAB 2016. Lecture Notes in Electrical Engineering, vol 444. Springer, Singapore. https://doi.org/10.1007/978-981-10-4801-2_47
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DOI: https://doi.org/10.1007/978-981-10-4801-2_47
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